scholarly journals Nasal epithelial barrier dysfunction increases sensitization and mast cell degranulation in the absence of allergic inflammation

Allergy ◽  
2019 ◽  
Vol 75 (5) ◽  
pp. 1155-1164 ◽  
Author(s):  
Inge Kortekaas Krohn ◽  
Sven F. Seys ◽  
Gitte Lund ◽  
Anne‐Charlotte Jonckheere ◽  
Isabelle Dierckx de Casterlé ◽  
...  
Keyword(s):  
Mast Cell ◽  
Allergic Inflammation ◽  
Mast Cell Degranulation ◽  
Epithelial Barrier ◽  
Barrier Dysfunction ◽  
Epithelial Barrier Dysfunction

scholarly journals Intestinal Epithelial Barrier Dysfunction in Food Hypersensitivity

2012 ◽  
Vol 2012 ◽  
pp. 1-11 ◽  
Author(s):  
Linda Chia-Hui Yu
Keyword(s):  
Mast Cell ◽  
Cell Surface ◽  
Critical Role ◽  
Mast Cell Activation ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Barrier Dysfunction ◽  
Intestinal Epithelial Barrier ◽  
Food Antigens ◽  
Epithelial Barrier Dysfunction

Intestinal epithelial barrier plays a critical role in the maintenance of gut homeostasis by limiting the penetration of luminal bacteria and dietary allergens, yet allowing antigen sampling for the generation of tolerance. Undigested proteins normally do not gain access to the lamina propria due to physical exclusion by tight junctions at the cell-cell contact sites and intracellular degradation by lysosomal enzymes in enterocytes. An intriguing question then arises: how do macromolecular food antigens cross the epithelial barrier? This review discusses the epithelial barrier dysfunction in sensitized intestine with special emphasis on the molecular mechanism of the enhanced transcytotic rates of allergens. The sensitization phase of allergy is characterized by antigen-induced cross-linking of IgE bound to high affinity FcεRI on mast cell surface, leading to anaphylactic responses. Recent studies have demonstrated that prior to mast cell activation, food allergens are transported in large quantity across the epithelium and are protected from lysosomal degradation by binding to cell surface IgE and low-affinity receptor CD23/FcεRII. Improved immunotherapies are currently under study including anti-IgE and anti-CD23 antibodies for the management of atopic disorders.

scholarly journals Chymase-mediated intestinal epithelial permeability is regulated by a protease-activating receptor/matrix metalloproteinase-2-dependent mechanism

2013 ◽  
Vol 304 (5) ◽  
pp. G479-G489 ◽  
Author(s):  
Katherine R. Groschwitz ◽  
David Wu ◽  
Heather Osterfeld ◽  
Richard Ahrens ◽  
Simon P. Hogan
Keyword(s):  
Mast Cell ◽  
Mast Cells ◽  
Barrier Function ◽  
Epithelial Barrier ◽  
Intestinal Epithelial ◽  
Barrier Dysfunction ◽  
Intestinal Epithelial Barrier ◽  
Epithelial Barrier Function ◽  
Epithelial Barrier Dysfunction

Mast cells regulate intestinal barrier function during disease and homeostasis. Secretion of the mast cell-specific serine protease chymase regulates homeostasis. In the present study, we employ in vitro model systems to delineate the molecular pathways involved in chymase-mediated intestinal epithelial barrier dysfunction. Chymase stimulation of intestinal epithelial (Caco-2 BBe) cell monolayers induced a significant reduction in transepithelial resistance, indicating decreased intestinal epithelial barrier function. The chymase-induced intestinal epithelial barrier dysfunction was characterized by chymase-induced protease-activated receptor (PAR)-2 activation and matrix metalloproteinase (MMP)-2 expression and activation. Consistent with this observation, in vitro analysis revealed chymase-induced PAR-2 activation and increased MAPK activity and MMP-2 expression. Pharmacological and small interfering RNA-mediated antagonism of PAR-2 and MMP-2 significantly attenuated chymase-stimulated barrier dysfunction. Additionally, the chymase/MMP-2-mediated intestinal epithelial dysfunction was associated with a significant reduction in the tight junction protein claudin-5, which was partially restored by MMP-2 inhibition. Finally, incubation of Caco-2 BBe cells with chymase-sufficient, but not chymase-deficient, bone marrow-derived mast cells decreased barrier function, which was attenuated by the chymase inhibitor chymostatin. Collectively, these results suggest that mast cell/chymase-mediated intestinal epithelial barrier function is mediated by PAR-2/MMP-2-dependent pathways.

scholarly journals Tripterine alleviates lipopolysaccharide-induced airway epithelial barrier dysfunction through suppressing the Hippo pathway

RSC Advances ◽  
2018 ◽  
Vol 8 (69) ◽  
pp. 39696-39702 ◽  
Author(s):  
Jie Gao ◽  
Wenying Wang
Keyword(s):  
Hippo Pathway ◽  
Allergic Inflammation ◽  
Epithelial Barrier ◽  
Airway Epithelial ◽  
Barrier Dysfunction ◽  
Epithelial Barrier Dysfunction ◽  
The Hippo Pathway

Recent studies show that airway epithelial barrier dysfunction is closely associated with allergic inflammation and asthma pathogenesis.

Mast cell activation is involved in stress-induced epithelial barrier dysfunction in the esophagus

2015 ◽  
Vol 16 (4) ◽  
pp. 186-196 ◽  
Author(s):  
Chan Juan Zhong ◽  
Kun Wang ◽  
Lu Zhang ◽  
Chang Qing Yang ◽  
Kuo Zhang ◽  
...  
Keyword(s):  
Mast Cell ◽  
Cell Activation ◽  
Mast Cell Activation ◽  
Epithelial Barrier ◽  
Barrier Dysfunction ◽  
Epithelial Barrier Dysfunction

NAD+ Ameliorates Inflammation-Induced Epithelial Barrier Dysfunction in Cultured Enterocytes and Mouse Ileal Mucosa

2003 ◽  
Vol 307 (2) ◽  
pp. 443-449 ◽  
Author(s):  
Xiaonan Han ◽  
Takashi Uchiyama ◽  
Penny L. Sappington ◽  
Arino Yaguchi ◽  
Runkuan Yang ◽  
...  
Keyword(s):  
Epithelial Barrier ◽  
Barrier Dysfunction ◽  
Ileal Mucosa ◽  
Epithelial Barrier Dysfunction

Correlation between cyclical epithelial barrier dysfunction and bacterial translocation in the relapses of intestinal inflammation

2006 ◽  
Vol 12 (9) ◽  
pp. 843-852 ◽  
Author(s):  
Monica Porras ◽  
Maria Teresa Martín ◽  
Ping-Chang Yang ◽  
Jennifer Jury ◽  
Mary H. Perdue ◽  
...  
Keyword(s):  
Bacterial Translocation ◽  
Intestinal Inflammation ◽  
Epithelial Barrier ◽  
Barrier Dysfunction ◽  
Epithelial Barrier Dysfunction
Sign in / Sign up

Export Citation Format

Share Document