scholarly journals Phage display peptide libraries in molecular allergology: from epitope mapping to mimotope-based immunotherapy

Allergy ◽  
2016 ◽  
Vol 71 (11) ◽  
pp. 1526-1532 ◽  
Author(s):  
J. Luzar ◽  
B. Štrukelj ◽  
M. Lunder
Keyword(s):  
Phage Display ◽  
Epitope Mapping ◽  
Peptide Libraries ◽  
Molecular Allergology

scholarly journals SAROTUP: Scanner and Reporter of Target-Unrelated Peptides

2010 ◽  
Vol 2010 ◽  
pp. 1-7 ◽  
Author(s):  
Jian Huang ◽  
Beibei Ru ◽  
Shiyong Li ◽  
Hao Lin ◽  
Feng-Biao Guo
Keyword(s):  
Phage Display ◽  
Epitope Mapping ◽  
Surface Display ◽  
Epitope Prediction ◽  
Peptide Libraries ◽  
Screening System ◽  
Web Tool ◽  
Random Peptide ◽  
Random Peptide Libraries ◽  
Convenient Approach

As epitope mimics, mimotopes have been widely utilized in the study of epitope prediction and the development of new diagnostics, therapeutics, and vaccines. Screening the random peptide libraries constructed with phage display or any other surface display technologies provides an efficient and convenient approach to acquire mimotopes. However, target-unrelated peptides creep into mimotopes from time to time through binding to contaminants or other components of the screening system. In this study, we present SAROTUP, a free web tool for scanning, reporting and excluding possible target-unrelated peptides from real mimotopes. Preliminary tests show that SAROTUP is efficient and capable of improving the accuracy of mimotope-based epitope mapping. It is also helpful for the development of mimotope-based diagnostics, therapeutics, and vaccines.

Phage display antibodies against ectromelia virus that neutralize variola virus: Selection and implementation for p35 neutralizing epitope mapping

2018 ◽  
Vol 152 ◽  
pp. 18-25 ◽  
Author(s):  
Yana Khlusevich ◽  
Andrey Matveev ◽  
Ivan Baykov ◽  
Leonid Bulychev ◽  
Nikolai Bormotov ◽  
...  
Keyword(s):  
Phage Display ◽  
Epitope Mapping ◽  
Variola Virus ◽  
Ectromelia Virus ◽  
Neutralizing Epitope

scholarly journals Epitope Mapping of Antibodies against Prostate-specific Antigen with Use of Peptide Libraries

2002 ◽  
Vol 48 (12) ◽  
pp. 2208-2216 ◽  
Author(s):  
Jari Leinonen ◽  
Ping Wu ◽  
Ulf-Håkan Stenman
Keyword(s):  
Prostate Specific Antigen ◽  
Cyclic Peptides ◽  
Epitope Mapping ◽  
Specific Antigen ◽  
Peptide Libraries ◽  
Sequence Motifs ◽  
Large Panel ◽  
Distinct Sequence ◽  
Binding Peptides ◽  
Important Marker

Abstract Background: Prostate-specific antigen (PSA) is the most important marker for prostate cancer, but PSA concentrations determined by various assays can differ significantly because of differences in specificity of the antibodies used. To identify epitopes recognized by various monoclonal antibodies (MAbs) to PSA, we have isolated peptides that react with the paratopes of these. Methods: Six anti-PSA MAbs representing three major epitope groups were screened with five cyclic phage display peptide libraries. After selection, the peptide sequences were determined by sequencing of the relevant part of viral DNA. Binding of the phage peptides to the MAbs was monitored by immunoassay. Results: For each MAb, several paratope-binding peptides with distinct sequence motifs were identified, but only ∼10% showed similarity with the PSA sequence. Some of these correctly predicted the location of the epitopes. By sequential panning of the library with two closely related MAbs, we identified peptides reacting equally with both MAbs. When analyzed against a large panel of PSA MAbs, the peptides generally showed restricted specificity toward the MAb used for selection, but some peptides bound to several related MAbs. Conclusions: Most of the cyclic peptides selected with PSA MAbs are specific for the MAb used for selection and do not resemble any sequence on the antigen. Peptides reactive with two MAbs recognizing the same epitope can be obtained by sequential panning. This method can be used to predict the location of some epitopes, but additional methods are needed to confirm the result.

Mimotopes of polyreactive anti-DNA antibodies identified using phage-display peptide libraries

1997 ◽  
Vol 27 (5) ◽  
pp. 1221-1228 ◽  
Author(s):  
Pierre Sibille ◽  
Thérèse Ternynck ◽  
Faridabano Nato ◽  
Gérard Buttin ◽  
Donny Strosberg ◽  
...  
Keyword(s):  
Phage Display ◽  
Peptide Libraries ◽  
Dna Antibodies

scholarly journals Phage display revisited: Epitope mapping of a monoclonal antibody directed againstNeisseria meningitidisadhesin A using the PROFILER technology

mAbs ◽  
2016 ◽  
Vol 8 (4) ◽  
pp. 741-750 ◽  
Author(s):  
Veronica Lanza Cariccio ◽  
Maria Domina ◽  
Salvatore Benfatto ◽  
Mario Venza ◽  
Isabella Venza ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Phage Display ◽  
Epitope Mapping

scholarly journals Epitope mapping using combinatorial phage-display libraries: a graph-based algorithm

2006 ◽  
Vol 35 (1) ◽  
pp. 69-78 ◽  
Author(s):  
Itay Mayrose ◽  
Tomer Shlomi ◽  
Nimrod D. Rubinstein ◽  
Jonathan M. Gershoni ◽  
Eytan Ruppin ◽  
...  
Keyword(s):  
Phage Display ◽  
Epitope Mapping ◽  
Phage Display Libraries

Epitope Mapping of Monoclonal Antibodies to Keratin 19 Using Keratin Fragments, Synthetic Peptides and Phage Peptide Libraries

1995 ◽  
Vol 231 (2) ◽  
pp. 475-485 ◽  
Author(s):  
Volker Bottger ◽  
Pauline C. Stasiak ◽  
Dolores L. Harrison ◽  
Dervla M. Mellerick ◽  
E. Birgitte Lane
Keyword(s):  
Monoclonal Antibodies ◽  
Synthetic Peptides ◽  
Epitope Mapping ◽  
Peptide Libraries ◽  
Keratin 19
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