Eosinophil major basic protein activates human cord blood mast cells primed with fibroblast membranes by integrin-β1

Allergy ◽  
2013 ◽  
Vol 68 (10) ◽  
pp. 1259-1268 ◽  
Author(s):  
M. Ben-Zimra ◽  
I. Bachelet ◽  
M. Seaf ◽  
G. J. Gleich ◽  
F. Levi-Schaffer
Keyword(s):  
Mast Cells ◽  
Cord Blood ◽  
Basic Protein ◽  
Major Basic Protein ◽  
Integrin Β1 ◽  
Human Cord Blood ◽  
Eosinophil Major Basic Protein

Non-IgE–dependent activation of human lung– and cord blood–derived mast cells is induced by eosinophil major basic protein and modulated by the membrane form of stem cell factor

Blood ◽  
2003 ◽  
Vol 101 (5) ◽  
pp. 1898-1904 ◽  
Author(s):  
Adrian M. Piliponsky ◽  
Gerald J. Gleich ◽  
Arnon Nagler ◽  
Ilan Bar ◽  
Francesca Levi-Schaffer
Keyword(s):  
Mast Cell ◽  
Stem Cell ◽  
Mast Cells ◽  
Cord Blood ◽  
Stem Cell Factor ◽  
Basic Protein ◽  
Human Lung ◽  
Late Phase ◽  
Major Basic Protein ◽  
Eosinophil Major Basic Protein

The allergic reaction begins with the antigen-induced aggregation of occupied high-affinity IgE receptors expressed on mast cell surface, their activation, and the release of proinflammatory mediators that cause the “early phase” of this process. In addition, mast cell activation induces the onset of a “late phase” reaction characterized by the tissue infiltration of inflammatory cells, mainly eosinophils. We have hypothesized that during the late phase mast cells interact with and are activated by eosinophils. Here we report that highly purified human lung mast cells became responsive to eosinophil major basic protein (MBP) when in coculture with human lung fibroblasts. In addition, cord blood–derived mast cells maintained in coculture with 3T3 fibroblasts released more histamine and prostaglandin D2 (PGD2) compared with cells maintained in suspension. The fibroblast-derived membrane form of stem cell factor (SCF) was found to be involved in the mast cell increased responsiveness to MBP. In fact, cord blood–derived mast cells cocultured with 3T3 in the presence of antisense for SCF or cocultured with fibroblasts that do not express the membrane form of SCF were inhibited in their histamine-releasing activity toward MBP. In addition, this form of SCF induced the expression of a pertussis toxin–sensitive Gi protein, Gi3 that interacts with MBP to trigger mast cell non-IgE–dependent activation in a manner similar to other cationic compounds such as compound 48/80. Mast cell responsiveness to eosinophil mediators is a potentially novel evidence for an alternative pathway of allergen-independent activation able to contribute to the perpetuation of allergy.

The proform of eosinophil major basic protein: a new maternal serum marker for Down syndrome

1999 ◽  
Vol 19 (10) ◽  
pp. 905-910 ◽  
Author(s):  
Michael Christiansen ◽  
Claus Oxvig ◽  
Jill M. Wagner ◽  
Qiu-Ping Qin ◽  
Tri H. Nguyen ◽  
...  
Keyword(s):  
Down Syndrome ◽  
Basic Protein ◽  
Protein A ◽  
Serum Marker ◽  
Maternal Serum ◽  
Major Basic Protein ◽  
Eosinophil Major Basic Protein

The Effect of Purified Human Eosinophil Major Basic Protein on Mammalian Ciliary Activity1–3

1987 ◽  
Vol 135 (4) ◽  
pp. 848-853 ◽  
Author(s):  
Annette T. Hastie ◽  
David A. Loegering ◽  
Gerald J. Gleich ◽  
Friedrich Kueppers
Keyword(s):  
Basic Protein ◽  
Major Basic Protein ◽  
Human Eosinophil ◽  
Eosinophil Major Basic Protein

Localization of eosinophils to airway nerves and effect on neuronal M2 muscarinic receptor function

1997 ◽  
Vol 273 (1) ◽  
pp. L93-L103 ◽  
Author(s):  
R. W. Costello ◽  
B. H. Schofield ◽  
G. M. Kephart ◽  
G. J. Gleich ◽  
D. B. Jacoby ◽  
...  
Keyword(s):  
Guinea Pigs ◽  
Acetylcholine Release ◽  
Basic Protein ◽  
Receptor Function ◽  
Major Basic Protein ◽  
Parasympathetic Nerves ◽  
M2 Muscarinic Receptor ◽  
Fatal Asthma ◽  
Eosinophil Major Basic Protein ◽  
M2 Muscarinic Receptors

Neuronal M2 muscarinic receptors inhibit acetylcholine release from pulmonary parasympathetic nerves but are dysfunctional in antigen-challenged animals and asthmatics. Deletion of pulmonary eosinophils protects M2 receptor function in antigen-challenged guinea pigs. Therefore, the association of eosinophils with airway nerves was investigated. Nerve-associated eosinophils were significantly increased in challenged animals compared with controls (0.75 +/- 0.05 vs. 0.28 +/- 0.05 eosinophils/nerve). In antigen-challenged animals, eosinophil density was greatest around airway nerves, suggesting recruitment to the nerves. M2 receptor function was inversely correlated with the number of eosinophils per nerve, thus eosinophils are associated with airway nerves in antigen-challenged guinea pigs, where they impair M2 receptor function. In airways from three patients with fatal asthma, 196 of 637 eosinophils (30%) were associated with nerves, and release of eosinophil major basic protein was evident; conversely, in three control patients 1 of 11 (9%) eosinophils were in contact with nerves. Thus eosinophils and their granule proteins are also seen in association with airway nerves in patients with asthma.

Cloning of cDNA for rat eosinophil major basic protein

1995 ◽  
Vol 1264 (3) ◽  
pp. 261-264 ◽  
Author(s):  
Takeaki Nittoh ◽  
Masako Watanabe ◽  
Hiroshi Okayama ◽  
Satoru Misawa ◽  
Yoshiaki Isobe ◽  
...  
Keyword(s):  
Basic Protein ◽  
Major Basic Protein ◽  
Eosinophil Major Basic Protein

scholarly journals Stem cell factor-dependent human cord blood derived mast cells express α- and β-tryptase, heparin and chondroitin sulphate

Immunology ◽  
1996 ◽  
Vol 88 (2) ◽  
pp. 308-314 ◽  
Author(s):  
G. NILSSON ◽  
T. BLOM ◽  
I. HARVIMA ◽  
M. KUSCHE-GULLBERG ◽  
K. NILSSON ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mast Cells ◽  
Cord Blood ◽  
Stem Cell Factor ◽  
Chondroitin Sulphate ◽  
Human Cord Blood
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