scholarly journals Warm ischemia time influences human islet cell isolation yield when assessed as beta cell number but not as islet equivalent number

2021 ◽  
Author(s):  
Diedert Luc De Paep ◽  
Daniel Jacobs‐Tulleneers‐Thevissen
Keyword(s):  
Beta Cell ◽  
Islet Cell ◽  
Cell Number ◽  
Cell Isolation ◽  
Warm Ischemia ◽  
Human Islet ◽  
Warm Ischemia Time ◽  
Ischemia Time ◽  
Equivalent Number

scholarly journals Lower beta cell yield from donor pancreases after controlled circulatory death prevented by shortening acirculatory warm ischemia time and by using IGL-1 cold preservation solution

PLoS ONE ◽  
2021 ◽  
Vol 16 (5) ◽  
pp. e0251055
Author(s):  
Diedert L. De Paep ◽  
Freya Van Hulle ◽  
Zhidong Ling ◽  
Marian Vanhoeij ◽  
Jacques Pirenne ◽  
...  
Keyword(s):  
Beta Cell ◽  
Beta Cells ◽  
Cell Number ◽  
Warm Ischemia ◽  
Warm Ischemia Time ◽  
Cell Yield ◽  
Preservation Solution ◽  
Cold Preservation ◽  
Ischemia Time ◽  
Circulatory Death

Organs from donors after controlled circulatory death (DCD III) exhibit a higher risk for graft dysfunction due to an initial period of warm ischemia. This procurement condition can also affect the yield of beta cells in islet isolates from donor pancreases, and hence their use for transplantation. The present study uses data collected and generated by our Beta Cell Bank to compare the number of beta cells in isolates from DCD III (n = 141) with that from donors after brain death (DBD, n = 609), before and after culture, and examines the influence of donor and procurement variables. Beta cell number per DCD III-organ was significantly lower (58 x 106 versus 84 x 106 beta cells per DBD-organ; p < 0.001) but their purity (24% insulin positive cells) and insulin content (17 μg / 106 beta cells in DCD III-organs versus 19 μg / 106 beta cells in DBD-organs) were similar. Beta cell number correlated negatively with duration of acirculatory warm ischemia time above 10 min; for shorter acirculatory warm ischemia time, DCD III-organs did not exhibit a lower beta cell yield (74 x 106 beta cells). Use of Institut Georges Lopez-1 cold preservation solution instead of University of Wisconsin solution or histidine-tryptophan-ketoglutarate also protected against the loss in beta cell yield from DCD III-organs (86 x 106 for IGL-1 versus 54 x 106 and 65 x 106 beta cells respectively, p = 0.042). Multivariate analysis indicates that both limitation of acirculatory warm ischemia time and use of IGL-1 prevent the reduced beta cell yield in islet cell isolates from DCD III-organs.

scholarly journals LIMITING WARM ISCHEMIA TIME IMPROVES ISLET CELL GRAFTS FROM DONOR PANCREASES AFTER CONTROLLED CIRCULATORY DEATH

2020 ◽  
Vol 104 (S3) ◽  
pp. S561-S561
Author(s):  
Diedert L. De Paep ◽  
Zhidong Ling ◽  
Marian Vanhoeij ◽  
Jacques Pirenne ◽  
Daniel Pipeleers ◽  
...  
Keyword(s):  
Islet Cell ◽  
Warm Ischemia ◽  
Warm Ischemia Time ◽  
Ischemia Time ◽  
Circulatory Death

scholarly journals Human islet cell implants in a nude rat model of diabetes survive better in omentum than in liver with a positive influence of beta cell number and purity

Diabetologia ◽  
2010 ◽  
Vol 53 (8) ◽  
pp. 1690-1699 ◽  
Author(s):  
D. Jacobs-Tulleneers-Thevissen ◽  
K. Bartholomeus ◽  
K. Suenens ◽  
I. Vermeulen ◽  
Z. Ling ◽  
...  
Keyword(s):  
Beta Cell ◽  
Rat Model ◽  
Islet Cell ◽  
Cell Number ◽  
Positive Influence ◽  
Human Islet ◽  
Nude Rat

V218: Eliminating Knot Tying During Warm Ischemia Time for Laparoscopic Partial Nephrectomy: The Video

2004 ◽  
Vol 171 (4S) ◽  
pp. 57-58
Author(s):  
Marcelo A. Orvieto ◽  
Gary W. Chien ◽  
R. Matthew Galocy ◽  
Mitchell H. Sokoloff ◽  
Gregory P. Zagaja ◽  
...  
Keyword(s):  
Partial Nephrectomy ◽  
Warm Ischemia ◽  
Warm Ischemia Time ◽  
Laparoscopic Partial Nephrectomy ◽  
Knot Tying ◽  
Ischemia Time

Short recipient warm ischemia time improves outcomes in deceased donor liver transplantation

2021 ◽  
Author(s):  
Abbas Al‐Kurd ◽  
Toshihiro Kitajima ◽  
Khortnal Delvecchio ◽  
Mhd Tayseer Shamaa ◽  
Tommy Ivanics ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Deceased Donor ◽  
Warm Ischemia ◽  
Warm Ischemia Time ◽  
Donor Liver ◽  
Ischemia Time ◽  
Donor Liver Transplantation ◽  
Deceased Donor Liver Transplantation

The First Assistant Sparing Technique Robot-Assisted Partial Nephrectomy Decreases Warm Ischemia Time While Maintaining Good Perioperative Outcomes

2012 ◽  
Vol 26 (11) ◽  
pp. 1448-1453 ◽  
Author(s):  
William T. Berg ◽  
Chad R. Rich ◽  
Gina M. Badalato ◽  
Christopher M. Deibert ◽  
Chris O. Wambi ◽  
...  
Keyword(s):  
Partial Nephrectomy ◽  
Warm Ischemia ◽  
Warm Ischemia Time ◽  
Perioperative Outcomes ◽  
Robot Assisted ◽  
Ischemia Time

Influence of Warm Ischemia Time on Peripheral-Type Benzodiazepine Receptor: A New Aspect of the Role of Mitochondria

2007 ◽  
Vol 107 (1) ◽  
pp. e1-e11 ◽  
Author(s):  
Carole Doucet ◽  
Keqiang Zhang ◽  
Thibault Desurmont ◽  
William Hebrard ◽  
Michel Scepi ◽  
...  
Keyword(s):  
Benzodiazepine Receptor ◽  
Warm Ischemia ◽  
Warm Ischemia Time ◽  
Ischemia Time ◽  
Peripheral Type

Effect of Warm Ischemia Time During Laparoscopic Partial Nephrectomy on Early Postoperative Glomerular Filtration Rate

2009 ◽  
Vol 181 (6) ◽  
pp. 2438-2445 ◽  
Author(s):  
Guilherme Godoy ◽  
Vigneshwaran Ramanathan ◽  
Jamie A. Kanofsky ◽  
Rebecca L. O'Malley ◽  
Basir U. Tareen ◽  
...  
Keyword(s):  
Glomerular Filtration Rate ◽  
Glomerular Filtration ◽  
Partial Nephrectomy ◽  
Filtration Rate ◽  
Warm Ischemia ◽  
Warm Ischemia Time ◽  
Laparoscopic Partial Nephrectomy ◽  
Ischemia Time
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