scholarly journals Polyreactive Antibodies Developing Amidst Humoral Rejection of Human Kidney Grafts Bind Apoptotic Cells and Activate Complement

2013 ◽  
Vol 13 (10) ◽  
pp. 2590-2600 ◽  
Author(s):  
F. Porcheray ◽  
J. W. Fraser ◽  
B. Gao ◽  
A. McColl ◽  
J. DeVito ◽  
...  
Keyword(s):  
Human Kidney ◽  
Apoptotic Cells ◽  
Humoral Rejection ◽  
Polyreactive Antibodies

scholarly journals C4d Deposition after Allogeneic Renal Transplantation in Rats Is Involved in Initial Apoptotic Cell Clearance

Cells ◽  
2021 ◽  
Vol 10 (12) ◽  
pp. 3499
Author(s):  
Stefan Reuter ◽  
Dominik Kentrup ◽  
Alexander Grabner ◽  
Gabriele Köhler ◽  
Konrad Buscher ◽  
...  
Keyword(s):  
Complement Activation ◽  
Apoptotic Cell ◽  
Human Kidney ◽  
Positive Staining ◽  
Apoptotic Cells ◽  
Complement Factors ◽  
Antibody Mediated Rejection ◽  
Apoptotic Cell Clearance ◽  
Cell Clearance ◽  
Renal Transplantations

In the context of transplantation, complement activation is associated with poor prognosis and outcome. While complement activation in antibody-mediated rejection is well-known, less is known about complement activation in acute T cell-mediated rejection (TCMR). There is increasing evidence that complement contributes to the clearance of apoptotic debris and tissue repair. In this regard, we have analysed published human kidney biopsy transcriptome data clearly showing upregulated expression of complement factors in TCMR. To clarify whether and how the complement system is activated early during acute TCMR, experimental syngeneic and allogeneic renal transplantations were performed. Using an allogeneic rat renal transplant model, we also observed upregulation of complement factors in TCMR in contrast to healthy kidneys and isograft controls. While staining for C4d was positive, staining with a C3d antibody showed no C3d deposition. FACS analysis of blood showed the absence of alloantibodies that could have explained the C4d deposition. Gene expression pathway analysis showed upregulation of pro-apoptotic factors in TCMR, and apoptotic endothelial cells were detected by ultrastructural analysis. Monocytes/macrophages were found to bind to and phagocytise these apoptotic cells. Therefore, we conclude that early C4d deposition in TCMR may be relevant to the clearance of apoptotic cells.

scholarly journals Chronic Humoral Rejection of Human Kidney Allografts Is Associated with MMP-2 Accumulation in Podocytes and its Release in the Urine

2010 ◽  
Vol 10 (11) ◽  
pp. 2463-2471 ◽  
Author(s):  
W. Wong ◽  
J. DeVito ◽  
H. Nguyen ◽  
D. Sarracino ◽  
F. Porcheray ◽  
...  
Keyword(s):  
Human Kidney ◽  
Humoral Rejection

scholarly journals Chronic Humoral Rejection of Human Kidney Allografts Associates With Broad Autoantibody Responses

2010 ◽  
Vol 89 (10) ◽  
pp. 1239-1246 ◽  
Author(s):  
Fabrice Porcheray ◽  
Julie DeVito ◽  
Beow Y. Yeap ◽  
Lijuan Xue ◽  
Ian Dargon ◽  
...  
Keyword(s):  
Human Kidney ◽  
Humoral Rejection

1107: Evaluation of Cystine Transport in Cultured Human Kidney Cells: Primer to Gene Therapy of Cystinuria

2005 ◽  
Vol 173 (4S) ◽  
pp. 300-300
Author(s):  
Sreedhar Sagi ◽  
Lutz Trojan ◽  
Peter Aiken ◽  
Maurice S. Michel ◽  
Thomas Knoll
Keyword(s):  
Gene Therapy ◽  
Human Kidney ◽  
Kidney Cells

378: 14-3-3 Expression Patterns in the Human Kidney: From Fetal Development to the Adult State to Malignant Transformation

2005 ◽  
Vol 173 (4S) ◽  
pp. 103-103
Author(s):  
Adam G. Baseman ◽  
Andrew J. Kirsch ◽  
Fray F. Marshall ◽  
Haiyen E. Zhau ◽  
Leland W.K. Chung ◽  
...  
Keyword(s):  
Malignant Transformation ◽  
Fetal Development ◽  
Expression Patterns ◽  
Human Kidney

PROPERTIES OF SPECIFIC ESTROGEN-BINDING COMPONENTS IN HUMAN KIDNEY AND RENAL CARCINOMA

1975 ◽  
Vol 80 (1_Suppla) ◽  
pp. S51 ◽  
Author(s):  
H. Bojar ◽  
J. L. Wittliff ◽  
K. Balzer ◽  
R. Dreyfürst ◽  
F. Boeminghaus ◽  
...  
Keyword(s):  
Renal Carcinoma ◽  
Human Kidney ◽  
Estrogen Binding

Spatial Metabolomics of the Human Kidney Using MALDI Trapped Ion Mobility Imaging Mass Spectrometry

2020 ◽  
Author(s):  
Elizabeth Neumann ◽  
Lukasz Migas ◽  
Jamie L. Allen ◽  
Richard Caprioli ◽  
Raf Van de Plas ◽  
...  
Keyword(s):  
Mass Spectrometry ◽  
Ion Mobility ◽  
Imaging Mass Spectrometry ◽  
Structural Complexity ◽  
Human Kidney ◽  
Resolving Power ◽  
Mobility Separation ◽  
Trapped Ion ◽  
Mobility Data ◽  
Ion Mobility Separation

<div> <div> <p>Small metabolites are essential for normal and diseased biological function but are difficult to study because of their inherent structural complexity. MALDI imaging mass spectrometry (IMS) of small metabolites is particularly challenging as MALDI matrix clusters are often isobaric with metabolite ions, requiring high resolving power instrumentation or derivatization to circumvent this issue. An alternative to this is to perform ion mobility separation before ion detection, enabling the visualization of metabolites without the interference of matrix ions. Here, we use MALDI timsTOF IMS to image small metabolites at high spatial resolution within the human kidney. Through this, we have found metabolites, such as arginic acid, acetylcarnitine, and choline that localize to the cortex, medulla, and renal pelvis, respectively. We have also demonstrated that trapped ion mobility spectrometry (TIMS) can resolve matrix peaks from metabolite signal and separate both isobaric and isomeric metabolites with different localizations within the kidney. The added ion mobility data dimension dramatically increased the peak capacity for molecular imaging experiments. Future work will involve further exploring the small metabolite profiles of human kidneys as a function of age, gender, and ethnicity.</p></div></div>

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