Autophagy gene ATG5 knockdown upregulates apoptotic cell death during Candida albicans infection in human vaginal epithelial cells

2018 ◽  
Vol 80 (6) ◽  
pp. e13056 ◽  
Author(s):  
Ankit Shroff ◽  
Kudumula Venkata Rami Reddy
Keyword(s):  
Cell Death ◽  
Candida Albicans ◽  
Epithelial Cells ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Autophagy Gene ◽  
Vaginal Epithelial Cells ◽  
Candida Albicans Infection

scholarly journals Tritrichomonas foetus Induces Apoptotic Cell Death in Bovine Vaginal Epithelial Cells

2004 ◽  
Vol 72 (7) ◽  
pp. 4151-4158 ◽  
Author(s):  
B. N. Singh ◽  
J. J. Lucas ◽  
G. R. Hayes ◽  
Ish Kumar ◽  
D. H. Beach ◽  
...  
Keyword(s):  
Cell Death ◽  
Epithelial Cells ◽  
Molecular Mass ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Peptide Sequence ◽  
Mass Spectrometry Analysis ◽  
Tritrichomonas Foetus ◽  
Vaginal Epithelial Cells

ABSTRACT Tritrichomonas foetus is a serious veterinary pathogen, causing bovine trichomoniasis, a sexually transmitted disease leading to infertility and abortion. T. foetus infects the mucosal surfaces of the reproductive tract. Infection with T. foetus leads to apoptotic cell death of bovine vaginal epithelial cells (BVECs) in culture. An affinity-purified cysteine protease (CP) fraction yielding on sodium dodecyl sulfate-polyacrylamide gel electrophoresis a single band with an apparent molecular mass of 30 kDa (CP30) also induces BVEC apoptosis. Treatment of CP30 with the protease inhibitors TLCK (Nα-p-tosyl-l-lysine chloromethyl ketone) and E-64 [l-trans-epoxysuccinyl-leucylamide-(4-guanido)-butane] greatly reduces induction of BVEC apoptosis. Matrix-assisted laser desorption ionization-time-of-flight MALDI-TOF mass spectrometry analysis of CP30 reveals a single peak with a molecular mass of 23.7 kDa. Mass spectral peptide sequence analysis of proteolytically digested CP30 reveals homologies to a previously reported cDNA clone, CP8 (D. J. Mallinson, J. Livingstone, K. M. Appleton, S. J. Lees, G. H. Coombs, and M. J. North, Microbiology 141:3077-3085, 1995). Induction of apoptosis is highly species specific, since the related human parasite Trichomonas vaginalis and associated purified CPs did not induce BVEC death. Fluorescence microscopy along with the Cell Death Detection ELISAPLUS assay and flow cytometry analyses were used to detect apoptotic nuclear condensation, DNA fragmentation, and changes in plasma membrane asymmetry in host cells undergoing apoptosis in response to T. foetus infection or incubation with CP30. Additionally, the activation of caspase-3 and inhibition of cell death by caspase inhibitors indicates that caspases are involved in BVEC apoptosis. These results imply that apoptosis is involved in the pathogenesis of T. foetus infection in vivo, which may have important implications for therapeutic interference with host cell death that could alter the course of the pathology in vivo.

Uterine epithelial expression of the tumor necrosis factor superfamily: a strategy for immune privilege during pregnancy in a true epitheliochorial placentation species

2020 ◽  
Vol 102 (4) ◽  
pp. 828-842 ◽  
Author(s):  
Inkyu Yoo ◽  
Yoon Chul Kye ◽  
Jisoo Han ◽  
Minjeong Kim ◽  
Soohyung Lee ◽  
...  
Keyword(s):  
Cell Death ◽  
Tumor Necrosis Factor ◽  
Epithelial Cells ◽  
Tumor Necrosis ◽  
Fas Ligand ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Immune Privilege ◽  
Tumor Necrosis Factor Superfamily ◽  
Necrosis Factor

Abstract The maternal immune system tolerates semi-allogeneic placental tissues during pregnancy. Fas ligand (FASLG) and tumor necrosis factor superfamily 10 (TNFSF10) are known to be components of maternal immune tolerance in humans and mice. However, the role of FASLG and TNFSF10 in the tolerance process has not been studied in pigs, which form a true epitheliochorial type placenta. Thus, the present study examined the expression and function of FASLG and TNFSF10 and their receptors at the maternal-conceptus interface in pigs. The endometrium and conceptus tissues expressed FASLG and TNFSF10 and their receptor mRNAs during pregnancy in a stage-specific manner. During pregnancy, FASLG and TNFSF10 proteins were localized predominantly to endometrial luminal epithelial cells with strong signals on Day 30 to term and on Day 15, respectively, and receptors for TNFSF10 were localized to some stromal cells. Interferon-γ (IFNG) increased the expression of TNFSF10 and FAS in endometrial tissues. Co-culture of porcine endometrial epithelial cells over-expressing TNFSF10 with peripheral blood mononuclear cells yielded increased apoptotic cell death of lymphocytes and myeloid cells. In addition, many apoptotic T cells were found in the endometrium on Day 15 of pregnancy. The present study demonstrated that FASLG and TNFSF10 were expressed at the maternal-conceptus interface and conceptus-derived IFNG increased endometrial epithelial TNFSF10, which, in turn, induced apoptotic cell death of immune cells. These results suggest that endometrial epithelial FASLG and TNFSF10 may be critical for the formation of micro-environmental immune privilege at the maternal-conceptus interface for the establishment and maintenance of pregnancy in pigs.

Silver nanoparticles induce apoptotic cell death in Candida albicans through the increase of hydroxyl radicals

FEBS Journal ◽  
2012 ◽  
Vol 279 (7) ◽  
pp. 1327-1338 ◽  
Author(s):  
In-sok Hwang ◽  
Juneyoung Lee ◽  
Ji Hong Hwang ◽  
Keuk-Jun Kim ◽  
Dong Gun Lee
Keyword(s):  
Silver Nanoparticles ◽  
Cell Death ◽  
Candida Albicans ◽  
Hydroxyl Radicals ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death

scholarly journals Novel lnc RNA regulated by HIF-1 inhibits apoptotic cell death in the renal tubular epithelial cells under hypoxia

2017 ◽  
Vol 5 (8) ◽  
pp. e13203 ◽  
Author(s):  
Imari Mimura ◽  
Yosuke Hirakawa ◽  
Yasuharu Kanki ◽  
Natsuki Kushida ◽  
Ryo Nakaki ◽  
...  
Keyword(s):  
Cell Death ◽  
Epithelial Cells ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Tubular Epithelial Cells ◽  
Renal Tubular Epithelial Cells ◽  
Renal Tubular

Paraquat-Induced Apoptotic Cell Death in Lung Epithelial Cells

2006 ◽  
Vol 61 (4) ◽  
pp. 366 ◽  
Author(s):  
Tak Ho Song ◽  
Joo Yeon Yang ◽  
In Kook Jeong ◽  
Jae Seok Park ◽  
Young Koo Jee ◽  
...  
Keyword(s):  
Cell Death ◽  
Epithelial Cells ◽  
Apoptotic Cell ◽  
Apoptotic Cell Death ◽  
Lung Epithelial Cells ◽  
Lung Epithelial
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