Transcriptional profiling of primary endometrial epithelial cells following acute HIV-1 exposure reveals gene signatures related to innate immunity

2018 ◽  
Vol 79 (4) ◽  
pp. e12822 ◽  
Author(s):  
Muhammad Atif Zahoor ◽  
Matthew William Woods ◽  
Sara Dizzell ◽  
Aisha Nazli ◽  
Kristen M. Mueller ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Epithelial Cells ◽  
Transcriptional Profiling ◽  
Gene Signatures ◽  
Acute Hiv ◽  
Hiv 1 ◽  
Endometrial Epithelial Cells

scholarly journals Different effects of cortisol on pro-inflammatory gene expressions in LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells

2020 ◽  
Vol 16 (1) ◽  
Author(s):  
Luying Cui ◽  
Yali Wang ◽  
Heng Wang ◽  
Junsheng Dong ◽  
Zixiang Li ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Epithelial Cells ◽  
Dairy Cows ◽  
Bacterial Infections ◽  
Gene Expressions ◽  
Tlr4 Mrna ◽  
Tnf Α ◽  
Peripartum Period ◽  
Inflammatory Action ◽  
Endometrial Epithelial Cells

Abstract Background Bacterial infections are common in postpartum dairy cows. Cortisol level has been observed to increase in dairy cows during peripartum period, and is associated with the endometrial innate immunity against pathogens like E.coli. However, the mechanism underlying how cortisol regulates E.coli-induced inflammatory response in bovine endometrial epithelial cells (BEEC) remains elusive. Results Cortisol decreased the expressions of IL1β, IL6, TNF-α, IL8, and TLR4 mRNA in BEEC treated with LPS or heat-killed E.coli, but up-regulated these gene expressions in BEEC stimulated by live E.coli. Conclusion Cortisol exerted the anti-inflammatory action on LPS- or heat-killed E.coli-stimulated BEEC, but the pro-inflammatory action on live E.coli-induced BEEC.

scholarly journals Different effects of cortisol on pro-inflammatory gene expressions of LPS-, heat-killed E.coli-, or live E.coli-stimulated bovine endometrial epithelial cells

2019 ◽  
Author(s):  
Luying Cui ◽  
Yali Wang ◽  
Heng Wang ◽  
Junsheng Dong ◽  
Zixiang Li ◽  
...  
Keyword(s):  
Innate Immunity ◽  
Epithelial Cells ◽  
Dairy Cows ◽  
Bacterial Infections ◽  
Gene Expressions ◽  
Tlr4 Mrna ◽  
Tnf Α ◽  
Peripartum Period ◽  
Anti Inflammation ◽  
Endometrial Epithelial Cells

Abstract Background: Bacterial infections are common in postpartum dairy cows. Cortisol level has been observed to increase in dairy cows during peripartum period, and is associated with the endometrial innate immunity against pathogen like E.coli . However, the mechanism underlying how cortisol regulates E.coli -induced inflammatory response in bovine endometrial epithelial cells (BEEC) remains elusive. Results: Cortisol decreased the expressions of IL1β, IL6, TNF-α, IL8, and TLR4 mRNA in BEEC treated with LPS or heat-killed E.coli , but up-regulated the these gene expressions in BEEC stimulated by live E.coli . Conclusion: Cortisol exerted the anti-inflammation action on LPS- or heat-killed E.coli -stimulated BEEC, but the pro-inflammation action on live E.coli -induced BEEC.

scholarly journals Innate immunity in acute HIV-1 infection

2011 ◽  
Vol 6 (5) ◽  
pp. 353-363 ◽  
Author(s):  
Persephone Borrow
Keyword(s):  
Innate Immunity ◽  
Acute Hiv ◽  
Hiv 1

scholarly journals R5- and X4-HIV-1 use differentially the endometrial epithelial cells HEC-1A to ensure their own spread: Implication for mechanisms of sexual transmission

Virology ◽  
2007 ◽  
Vol 358 (1) ◽  
pp. 55-68 ◽  
Author(s):  
Héla Saïdi ◽  
Giuliana Magri ◽  
Nadine Nasreddine ◽  
Mary Réquena ◽  
Laurent Bélec
Keyword(s):  
Epithelial Cells ◽  
Sexual Transmission ◽  
Hiv 1 ◽  
Endometrial Epithelial Cells

scholarly journals Differential Expression of Wnt Signaling Molecules Between Pre- and Postmenopausal Endometrial Epithelial Cells Suggests a Population of Putative Epithelial Stem/Progenitor Cells Reside in the Basalis Layer

Endocrinology ◽  
2012 ◽  
Vol 153 (6) ◽  
pp. 2870-2883 ◽  
Author(s):  
Hong P. T. Nguyen ◽  
Carl N. Sprung ◽  
Caroline E. Gargett
Keyword(s):  
Epithelial Cells ◽  
Wnt Signaling ◽  
Differential Expression ◽  
Transcriptional Profiling ◽  
Wnt Signaling Pathway ◽  
Gene Families ◽  
Gene Profile ◽  
M Gene ◽  
Endometrial Epithelium ◽  
Endometrial Epithelial Cells

The human endometrium undergoes extensive monthly regeneration in response to fluctuating levels of circulating estrogen and progesterone in premenopausal (Pre-M) women. In contrast, postmenopausal (Post-M) endometrium is thin and quiescent with low mitotic activity, similar to the Pre-M endometrial basalis layer. Clonogenic epithelial stem/progenitor (ESP) cells, likely responsible for regenerating endometrial epithelium, have been identified in Pre-M and Post-M endometrium, but their location is unknown. We undertook transcriptional profiling of highly purified epithelial cells from full-thickness Pre-M and Post-M endometrium to identify differentially regulated genes that may indicate a putative ESP cell population resides in the basalis of Pre-M and basalis-like Post-M endometrium. Of 1077 differentially expressed genes identified, the Wnt signaling pathway, important in endometrial development and stem cell regulation, was one of the main gene families detected, including 22 Wnt-associated genes. Twelve genes were validated using quantitative RT-PCR, and all were concordant with microarray data. Immunostaining showed glandular epithelial location of Wnt-regulated genes, Axin-related protein 2 and β-catenin. Axin2 localized to the nucleus of basalis Pre-M and Post-M and cytoplasm of functionalis Pre-M endometrium, suggesting that it regulates β-catenin. Comparison of our Post-M gene profile with published gene microarray datasets revealed similarities to Pre-M basalis epithelial profiles. This differential expression of multiple Wnt-associated genes in human Pre-M and Post-M endometrial epithelial cells and the similar gene profile of Post-M and Pre-M basalis epithelium suggests that a population of putative endometrial ESP may reside in the basalis of Pre-M endometrium, which may be responsible for regenerating glandular epithelium each month.

Embryonic regulation of IL-8 production and secretion in human endometrial epithelial cells

1998 ◽  
Vol 5 (1) ◽  
pp. 117A-117A ◽  
Author(s):  
P CABALLEROCAMPO ◽  
A BERNAL ◽  
A MERCADER ◽  
E OCONNOR ◽  
J COLOMA ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Embryonic Regulation ◽  
Endometrial Epithelial Cells
Sign in / Sign up

Export Citation Format

Share Document