Circulating endothelial cells, circulating endothelial progenitor cells, and von Willebrand factor in pregnancies complicated by hypertensive disorders

2017 ◽  
Vol 77 (3) ◽  
pp. e12625 ◽  
Author(s):  
Agata Szpera-Goździewicz ◽  
Maciej Majcherek ◽  
Maciej Boruczkowski ◽  
Tomasz Goździewicz ◽  
Grzegorz Dworacki ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Von Willebrand Factor ◽  
Circulating Endothelial Cells ◽  
Endothelial Progenitor ◽  
Hypertensive Disorders ◽  
Circulating Endothelial Progenitor Cells ◽  
Von Willebrand ◽  
Willebrand Factor

Unresolved questions, changing definitions, and novel paradigms for defining endothelial progenitor cells

Blood ◽  
2005 ◽  
Vol 106 (5) ◽  
pp. 1525-1531 ◽  
Author(s):  
David A. Ingram ◽  
Noel M. Caplice ◽  
Mervin C. Yoder
Keyword(s):  
Endothelial Cells ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Circulating Endothelial Cells ◽  
Proliferative Potential ◽  
Endothelial Progenitor ◽  
Detailed Understanding ◽  
Circulating Endothelial Progenitor Cells

Abstract The field of vascular biology has been stimulated by the concept that circulating endothelial progenitor cells (EPCs) may play a role in neoangiogenesis (postnatal vasculogenesis). One problem for the field has been the difficulty in accurately defining an EPC. Likewise, circulating endothelial cells (CECs) are not well defined. The lack of a detailed understanding of the proliferative potential of EPCs and CECs has contributed to the controversy in identifying these cells and understanding their biology in vitro or in vivo. A novel paradigm using proliferative potential as one defining aspect of EPC biology suggests that a hierarchy of EPCs exists in human blood and blood vessels. The potential implications of this view in relation to current EPC definitions are discussed.

scholarly journals Circulating Endothelial Cells, Circulating Endothelial Progenitor Cells, and Circulating Microparticles in Type 1 Diabetes Mellitus

2019 ◽  
Vol 25 ◽  
pp. 107602961882531 ◽  
Author(s):  
Asmaa M. Zahran ◽  
Ismail L. Mohamed ◽  
Osama M. El Asheer ◽  
Deiaaeldin M. Tamer ◽  
Mohamed G. M. Abo-ELela ◽  
...  
Keyword(s):  
Endothelial Cells ◽  
Type 1 Diabetes ◽  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Circulating Endothelial Cells ◽  
Endothelial Progenitor ◽  
Circulating Endothelial Progenitor Cells ◽  
Diabetic Children ◽  
Circulating Microparticles

Background and Aim: Hyperglycemia in type 1 diabetes (T1D) is accompanied by endothelial cell dysfunction which is known to contribute to the pathogenesis of cardiovascular disorders. The aim of the current study was to explore the profile of circulating endothelial progenitor cells (EPCs), circulating endothelial cells (CECs), endothelial and platelet derived micropaticles (EMPs, PMPs) and total microparticles (TMPs), in T1D children in relation to each other and to the metabolic disorders accompanying T1D. Patients and Methods: Thirty T1D patients and 20 age and sex matched healthy volunteers were assessed for HbA1c level and lipid profile. Quantification of CECs, EPCs, TMPs, EMPs and PMPs was done by flow cytometry. Results: The mean levels of EMPs, PMPs, TMPs and CECs were significantly higher in diabetic children compared to controls. Meanwhile, the levels of EPCs were significantly lower in diabetic children compared to controls. Both PMPs and CECs showed the highest significant differences between patients and controls and their levels were directly related to HbA1c, total cholesterol, LDL and triglycerides. A moderate correlation was observed between the frequency of PMPs and CECs. EPCs revealed negative correlations with both LDL and triglycerides. TMPs were only related to LDL, while EMPs were only related to HbA1c. Conclusion: Although there is disturbance in the levels of EMPs, PMPs, TMPs, CECs and EPCs in type 1 diabetic children compared to the controls, only the levels of PMPs and CECs were closely affected by the poor glycemic control and dyslipidemia occurring in T1D; thus may contribute to a higher risk of cardiovascular diseases.

Generation of Proliferating Blood-Derived Endothelial Progenitor Cells Critically Depends on Culture Conditions and Extracellular Matrix Proteins.

Blood ◽  
2006 ◽  
Vol 108 (11) ◽  
pp. 3922-3922
Author(s):  
Marcus Stockschlaeder ◽  
Olga Shardakova ◽  
Bianca Weingarth ◽  
Volker Stoldt ◽  
Rudiger E. Scharf
Keyword(s):  
Progenitor Cells ◽  
Endothelial Progenitor Cells ◽  
Von Willebrand Factor ◽  
Peripheral Blood ◽  
Proliferative Potential ◽  
Type I ◽  
Adherent Cells ◽  
Endothelial Progenitor ◽  
Von Willebrand ◽  
Willebrand Factor

Abstract Endothelial progenitor cells (EPC), bone marrow derived cells circulating in adult peripheral blood, may play an important role in postnatal vasculogenesis. We have used two different culture conditions for the isolation and generation of EPC from peripheral blood. Mononuclear cells (MNC) were harvested from peripheral blood (PB) of healthy volunteers by Ficoll-density gradient centrifugation. In the first method according to Hill et al., NEJM2003, 348: 593, cells were plated at a density of 5x106 cells/well in fibronectin-coated 6-well plates and cultured in Endocult (CellSystems). After a preplating step of 48 hours, non-adherent cells were cultured in fibronectin-coated 24-well plates at a density of 1x106/well. Emerging colonies (cell mass composed of a central cord of round cells with elongated spindle-shaped cells sprouting at the periphery) were counted at day 5. In the second method according to Lin et al., J Clin Invest2000, 105: 71, cells were plated at a density of 10x106 cells/well in collagen type I-coated 6-well plates and cultured in EBM2-MV (Cambrex) medium containing 5% FCS, VEGF, bFGF, R3-IGF-1, hEGF, hydrocortisone, and ascorbic acid. Emerging colonies were counted at day 14–25. Colony cells were phenotypically analyzed on day 7, 14, 21, 28 (method 1), and between day 40 – 55 (method 2) for the following antigens: leukocyte markers CD45 and CD14, and endothelial markers CD31 (PE-CAM), CD105 (endoglin), von-Willebrand factor, and CD34. Furthermore, the proliferative potential was evaluated by counting and determining the replating efficiency. Using method 1, colonies appeared on day 4–5. They consisted of centrally located round cells with elongated spindle-shaped cells sprouting at the periphery. The colonies disappeared after day 8–10; after 4 to 5 weeks the spindle-shaped cells degenerated to foamy cells. FACS analysis of the cells on day 5, 12, and 21 showed strong expression of CD45 and CD14, weak expression of CD31, but no expression of CD 105, CD34, and von-Willebrand factor. Using method 2, we observed cells with a different morphology and growth-pattern in 50% of the wells between 14–25 days. These cells rapidly replicated to form colonies with a cobblestone-like appearance which later formed a confluent monolayer. The cells continued growing for more 60 days. By FACS analysis, these cells showed strong expression of CD31 and CD105, weak expession of CD34, and no expression of CD45 or CD14. The cells stained positive for von-Willebrand factor. Our results suggest that cells which were cultured according to method 1 (fibronectin-coated plates, preplating step, use of non-adherent cells) display an angiogenic macrophage-like phenotype. These cells have a low proliferative potential. In contrast, cells which were cultured according to method 2 (collagen-type I-coated plates, no preplating, adherent cells) have a high proliferative potential and display an endothelial phenotype with no coexpression of leukocyte antigens. Since transduction with retroviral vectors depends on replication of target cells, isolated late endothelial outgrowth cells (EOC) generated according to the second method will be used for genetic modification to enhance the vasculogenetic properties of endothelial progenitor cells.

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