Differentiation and characterization of rat adipose tissue mesenchymal stem cells into endothelial-like cells

2017 ◽  
Vol 47 (1) ◽  
pp. 11-20 ◽  
Author(s):  
V. Cannella ◽  
G. Piccione ◽  
R. Altomare ◽  
A. Marino ◽  
P. Di Marco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Rat Adipose Tissue

Isolation and characterization of exosomes from adipose tissue‐derived mesenchymal stem cells

2020 ◽  
Author(s):  
Elsa González‐Cubero ◽  
María Luisa González‐Fernández ◽  
Laura Gutiérrez‐Velasco ◽  
Eliezer Navarro‐Ramírez ◽  
Vega Villar‐Suárez
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Isolation And Characterization

scholarly journals Comparison of biological features of wild European rabbit mesenchymal stem cells derived from different tissues.

2021 ◽  
Author(s):  
Marta Díaz-de Frutos ◽  
Alexandra Calle ◽  
María Zamora-Ceballos ◽  
Juan Bárcena ◽  
Esther Blanco ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
European Rabbit ◽  
Isolation And Characterization ◽  
Expression Characteristic ◽  
Subcutaneous Adipose ◽  
Different Tissues

Although the European rabbit is an "endangered" species and a notorious biological model, the analysis and comparative characterization of new tissue sources of rabbit mesenchymal stem cells (rMSCs) has not been well studied. Here we report for the first time the isolation and characterization of rMSCs derived from an animal belonging to a natural rabbit population within the species native region. New rMSC lines were isolated from different tissues: oral mucosa (rOM-MSC), dermal skin (rDS-MSC), subcutaneous adipose tissue (rSCA-MSC), ovarian adipose tissue (rOA-MSC), oviduct (rO-MSC), and mammary gland (rMG­MSC). The six rMSC lines showed plastic adhesion with fibroblast-like morphology and were all shown to be positive for CD44 and CD29 expression (characteristic markers of MSCs), and negative for CD34 or CD45 expression. In terms of pluripotency features, all rMSC lines expressed NANOG, OCT4, and SOX2. Furthermore, all rMSC lines cultured under osteogenic, chondrogenic, and adipogenic conditions showed differentiation capacity. In conclusion, this study describes the isolation and characterization of new rabbit cell lines from different tissue origins, with a clear mesenchymal pattern. We show that rMSC do not exhibit differences in terms of morphological features, expression of the cell surface, and intracellular markers of pluripotency and in vitro differentiation capacities, attributable to their tissue of origin.

scholarly journals Canine colostrum exosomes: Characterization and influence on the canine mesenchymal stem cell secretory profile and fibroblast anti-oxidative capacity.

2020 ◽  
Author(s):  
Antonio J Villatoro ◽  
María del Carmen Martín-Astorga ◽  
Cristina Alcoholado ◽  
José Becerra
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Oxidative Capacity ◽  
Positive Expression ◽  
Species Activity ◽  
Adipose Tissue Development ◽  
Cell Mobilization ◽  
First Time

Abstract Background : Canine colostrum milk (CCM) is a specific secretion of the mammary gland that is fundamental for the survival of the newborn. CCM has many described components (immunoglobulins, proteins or fat), but its small vesicles, named exosomes, are largely unknown. Results : A characterization of CCM exosomes was performed. Exosomes were abundant in CCM and appeared with the characteristic cup-shaped morphology and well-defined round vesicles. The size distribution of exosomes was between 37 and 140 nm, and western blot analysis showed positive expression of specific exosomal markers. Proteomic analysis revealed a total of 826 proteins in exosome cargo. We also found that exosomes modified the proliferation and secretory profiles in canine mesenchymal stem cells derived from bone marrow (cBM-MSCs) and adipose tissue (cAd-MSCs). Additionally, CCM exosomes demonstrated a potent antioxidant effect on canine fibroblasts in culture. Conclusions : Our findings highlight, for the first time, the abundant presence of exosomes in CCM and their ability to interact with mesenchymal stem cells (MSCs). The addition of exosomes to two types of MSCs in culture resulted in specific secretory profiles with functions related to angiogenesis, migration and chemotaxis of immune cells. In particular, the cAd-MSCs secretory profile showed higher potential in adipose tissue development and neurogenesis, while cBM-MSC production was associated with immunity, cell mobilization and haematopoiesis. Finally, exosomes also presented antioxidant capacity on fibroblasts against reactive oxygen species activity within the cell, demonstrating their fundamental role in the development and maturation of dogs in the early stages of their life.

Effects of different doses of melatonin on rat adipose derived mesenchymal stem cells

2021 ◽  
Vol 0 (0) ◽  
Author(s):  
Nazlı Çil ◽  
Mutlu Yaka ◽  
Nazire Gül Neşet ◽  
Mücahit Seçme ◽  
Gülçin Abban Mete
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue ◽  
Cell Viability ◽  
Total Antioxidant Status ◽  
Stress Index ◽  
Male Rat ◽  
Dependent Manner ◽  
Stem Cell Treatment ◽  
Rat Adipose Tissue

Abstract Objectives Stem cell treatment is based on Melatonin which is crucial for lots of pathological and physiological pathways. Our aim is determining the most appropriate dose of melatonin affecting the rat adipose tissue mesenchymal stem cells. Methods Stem cells were isolated from male rat adipose tissue. Differentiation and characterization experiments were performed. Cell viability analyses in stem cells were used the XTT [2,3-Bis-(2-methoxy-4-Nitro-5-Sulfophenyl)-2H-Tetrazolium-5-Carboxanilide] assay. After 24 h incubation, different concentrations (0.5, 1, 5, 10, 50 µM) of extract were treated to the stem cells for 24 h, 48 and 72 h considering time and dose dependent manner. Total antioxidant status (TAS) and the total oxidant status (TOS) in control cells and melatonin treated cells (5, 10 µM) were determined Rel Assay commercial kits. Results In 24 h, melatonin increased cell viability in all groups. When we evaluate the effect of melatonin in 48 h, the most proliferation increase was seen at 5, 10 µM doses. When the total oxidant activity melatonin was found to be significantly lower in 5 and 10 µM dose groups of melatonin. Conclusions Melatonin increases the survivor of stem cells and the most effective dose is 5 and 10 µM. The reduction of the oxidative stress index as a result of treating melatonin to mesenchymal stem cells showed that melatonin is a powerful antioxidant for stem cells.

Further insights into the characterization of equine adipose tissue-derived mesenchymal stem cells

2011 ◽  
Vol 35 (6) ◽  
pp. 355-365 ◽  
Author(s):  
Oksana Raabe ◽  
Katja Shell ◽  
Antonia Würtz ◽  
Christine Maria Reich ◽  
Sabine Wenisch ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue

Isolation and immunophenotypic characterization of mesenchymal stem cells derived from equine species adipose tissue

2009 ◽  
Vol 132 (2-4) ◽  
pp. 303-306 ◽  
Author(s):  
Armando de Mattos Carvalho ◽  
Ana Liz Garcia Alves ◽  
Marjorie Assis Golim ◽  
Andrei Moroz ◽  
Carlos Alberto Hussni ◽  
...  
Keyword(s):  
Stem Cells ◽  
Mesenchymal Stem Cells ◽  
Adipose Tissue
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