In VitroExpression of the Extracellular Matrix Components Aggrecan, Collagen Types I and II by Articular Cartilage-Derived Chondrocytes

2016 ◽  
Vol 46 (1) ◽  
pp. 43-50 ◽  
Author(s):  
J. Schneevoigt ◽  
C. Fabian ◽  
C. Leovsky ◽  
J. Seeger ◽  
M. Bahramsoltani
Keyword(s):  
Extracellular Matrix ◽  
Articular Cartilage ◽  
Collagen Types ◽  
Extracellular Matrix Components ◽  
Matrix Components

scholarly journals Alterations of Extracellular Matrix Components in the Course of Juvenile Idiopathic Arthritis

Metabolites ◽  
2021 ◽  
Vol 11 (3) ◽  
pp. 132
Author(s):  
Magdalena Wojdas ◽  
Klaudia Dąbkowska ◽  
Katarzyna Winsz-Szczotka
Keyword(s):  
Extracellular Matrix ◽  
Juvenile Idiopathic Arthritis ◽  
Articular Cartilage ◽  
Connective Tissue Diseases ◽  
Diagnostic Tools ◽  
Radiographic Imaging ◽  
Extracellular Matrix Components ◽  
Common Group ◽  
Matrix Components ◽  
And Function

Juvenile idiopathic arthritis (JIA) is the most common group of chronic connective tissue diseases in children that is accompanied by joint structure and function disorders. Inflammation underlying the pathogenic changes in JIA, caused by hypersecretion of proinflammatory cytokines, leads to the destruction of articular cartilage. The degradation which progresses with the duration of JIA is not compensated by the extent of repair processes. These disorders are attributed in particular to changes in homeostasis of extracellular matrix (ECM) components, including proteoglycans, that forms articular cartilage. Changes in metabolism of matrix components, associated with the disturbance of their degradation and biosynthesis processes, are the basis of the progressive wear of joint structures observed in the course of JIA. Clinical evaluation and radiographic imaging are current methods to identify the destruction. The aim of this paper is to review enzymatic and non-enzymatic factors involved in catabolism of matrix components and molecules stimulating their biosynthesis. Therefore, we discuss the changes in these factors in body fluids of children with JIA and their potential diagnostic use in the assessment of disease activity. Understanding the changes in ECM components in the course of the child-hood arthritis may provide the introduction of both new diagnostic tools and new therapeutic strategies in children with JIA.

Depth Dependent Diffusivity Profile in Bovine Articular Cartilage: Comparing Transverse and Axial Diffusivities

2007 ◽  
Author(s):  
Onyi N. Irrechukwu ◽  
Marc E. Levenston
Keyword(s):  
Extracellular Matrix ◽  
Articular Cartilage ◽  
Articular Surface ◽  
Deep Zone ◽  
Superficial Zone ◽  
Transport Pathway ◽  
Bovine Articular Cartilage ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Oriented Parallel

As articular cartilage is avascular, diffusion at a tissue length scale is the primary mode of solute and nutrient transport to its cells. The major extracellular matrix components are water (70–80%), chondrocytes, collagen (10–20%) and proteoglycans (5–10%) bearing sulfated glycosaminoglycans (GAG) [1]. Electron microscopy studies have shown that articular cartilage can be regarded as having three separate structural zones — superficial, middle and deep. The proportions of the various matrix components vary from the surface to the deep zone in any given joint and the greatest variations in content occur in the GAG content [2]. In addition the collagen fiber alignment varies, with fibers oriented parallel to the articular surface in the superficial zone, randomly oriented in the middle zone and oriented perpendicular to the surface in the deep zone. To a large extent, it is the spatially inhomogeneous composition of articular cartilage and microstructural orientation of its extracellular matrix components that determines the tortuosity of the transport pathway [3]. We therefore hypothesized that the diffusivity profile of a solute through the cartilage depth is inversely related to the GAG content and that the ratio between the axial and lateral diffusivities within each cartilage zone is related to the degree of anisotropy within the zone.

scholarly journals Age related extracellular matrix and interstitial cell phenotype in pulmonary valves

2020 ◽  
Vol 10 (1) ◽  
Author(s):  
Shaohua Wu ◽  
Vikas Kumar ◽  
Peng Xiao ◽  
Mitchell Kuss ◽  
Jung Yul Lim ◽  
...  
Keyword(s):  
Mechanical Properties ◽  
Extracellular Matrix ◽  
Heart Valve ◽  
Cell Phenotype ◽  
Primary Concern ◽  
Ross Operation ◽  
Age Related ◽  
Cardiovascular Morbidity And Mortality ◽  
Extracellular Matrix Components ◽  
Matrix Components

AbstractHeart valve disease is a common manifestation of cardiovascular disease and is a significant cause of cardiovascular morbidity and mortality worldwide. The pulmonary valve (PV) is of primary concern because of its involvement in common congenital heart defects, and the PV is usually the site for prosthetic replacement following a Ross operation. Although effects of age on valve matrix components and mechanical properties for aortic and mitral valves have been studied, very little is known about the age-related alterations that occur in the PV. In this study, we isolated PV leaflets from porcine hearts in different age groups (~ 4–6 months, denoted as young versus ~ 2 years, denoted as adult) and studied the effects of age on PV leaflet thickness, extracellular matrix components, and mechanical properties. We also conducted proteomics and RNA sequencing to investigate the global changes of PV leaflets and passage zero PV interstitial cells in their protein and gene levels. We found that the size, thickness, elastic modulus, and ultimate stress in both the radial and circumferential directions and the collagen of PV leaflets increased from young to adult age, while the ultimate strain and amount of glycosaminoglycans decreased when age increased. Young and adult PV had both similar and distinct protein and gene expression patterns that are related to their inherent physiological properties. These findings are important for us to better understand the physiological microenvironments of PV leaflet and valve cells for correctively engineering age-specific heart valve tissues.

Production of Extracellular Matrix Components in Tissue-Engineered Blood Vessels

2006 ◽  
Vol 12 (4) ◽  
pp. 831-842 ◽  
Author(s):  
Sepideh Heydarkhan-Hagvall ◽  
Maricris Esguerra ◽  
Gisela Helenius ◽  
Rigmor Söderberg ◽  
Bengt R. Johansson ◽  
...  
Keyword(s):  
Extracellular Matrix ◽  
Blood Vessels ◽  
Extracellular Matrix Components ◽  
Tissue Engineered Blood Vessels ◽  
Matrix Components

Guided differentiation of bone marrow stromal cells on co-cultured cartilage and bone scaffolds

Soft Matter ◽  
2015 ◽  
Vol 11 (38) ◽  
pp. 7648-7655 ◽  
Author(s):  
Paul Lee ◽  
Katelyn Tran ◽  
Gan Zhou ◽  
Asheesh Bedi ◽  
Namdev B. Shelke ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Extracellular Matrix ◽  
Stromal Cells ◽  
Bone Marrow Stromal Cells ◽  
Marrow Stromal Cells ◽  
Bone Scaffolds ◽  
Extracellular Matrix Components ◽  
Matrix Components ◽  
Osteochondral Tissue ◽  
Tissue Material

A biphasic micro and nanostructured scaffold with hydroxyapatite and extracellular matrix components was created for the regeneration of osteochondral tissue. Material cues of the biphasic scaffold supported differentiation of bone marrow stromal cells in both osteogenic and chondrogenic lineages.

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