Simulated validation of intron‐less transgene detection using DELLY for gene‐doping control in horse sports

2021 ◽  
Author(s):  
T. Tozaki ◽  
A. Ohnuma ◽  
M. Kikuchi ◽  
T. Ishige ◽  
H. Kakoi ◽  
...  
Keyword(s):  
Doping Control ◽  
Gene Doping ◽  
Transgene Detection

Low‐copy transgene detection using nested digital PCR for gene doping control

2021 ◽  
Author(s):  
Teruaki Tozaki ◽  
Aoi Ohnuma ◽  
Natasha A. Hamilton ◽  
Mio Kikuchi ◽  
Taichiro Ishige ◽  
...  
Keyword(s):  
Digital Pcr ◽  
Doping Control ◽  
Gene Doping ◽  
Transgene Detection

Robustness of Digital PCR and Real-Time PCR in Transgene Detection for Gene-Doping Control

2021 ◽  
Author(s):  
Teruaki Tozaki ◽  
Aoi Ohnuma ◽  
Shinichi Iwai ◽  
Mio Kikuchi ◽  
Taichiro Ishige ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Digital Pcr ◽  
Doping Control ◽  
Gene Doping ◽  
Transgene Detection

scholarly journals Whole-genome resequencing using genomic DNA extracted from horsehair roots for gene-doping control in horse sports

2020 ◽  
Vol 31 (4) ◽  
pp. 75-83
Author(s):  
Teruaki TOZAKI ◽  
Aoi OHNUMA ◽  
Mio KIKUCHI ◽  
Taichiro ISHIGE ◽  
Hironaga KAKOI ◽  
...  
Keyword(s):  
Genomic Dna ◽  
Doping Control ◽  
Whole Genome ◽  
Genome Resequencing ◽  
Gene Doping ◽  
Whole Genome Resequencing

scholarly journals Gene Doping Control in Horseracing Industries

2021 ◽  
Vol 49 (1) ◽  
pp. 19-29
Author(s):  
Teruaki TOZAKI ◽  
Aoi OHNUMA ◽  
Mio KIKUCHI ◽  
Taichiro ISHIGE ◽  
Kei-ichi HIROTA ◽  
...  
Keyword(s):  
Doping Control ◽  
Gene Doping

scholarly journals Microfluidic Quantitative PCR Detection of 12 Transgenes from Horse Plasma for Gene Doping Control

Genes ◽  
2020 ◽  
Vol 11 (4) ◽  
pp. 457 ◽  
Author(s):  
Teruaki Tozaki ◽  
Aoi Ohnuma ◽  
Mio Kikuchi ◽  
Taichiro Ishige ◽  
Hironaga Kakoi ◽  
...  
Keyword(s):  
Quantitative Pcr ◽  
Limit Of Detection ◽  
Doping Control ◽  
Taqman Probe ◽  
Specific Detection ◽  
Gene Doping ◽  
Athletic Ability ◽  
Horse Plasma ◽  
Fluidic Circuits ◽  
Primer Sets

Gene doping, an activity which abuses and misuses gene therapy, is a major concern in sports and horseracing industries. Effective methods capable of detecting and monitoring gene doping are urgently needed. Although several PCR-based methods that detect transgenes have been developed, many of them focus only on a single transgene. However, numerous genes associated with athletic ability may be potential gene-doping material. Here, we developed a detection method that targets multiple transgenes. We targeted 12 genes that may be associated with athletic performance and designed two TaqMan probe/primer sets for each one. A panel of 24 assays was prepared and detected via a microfluidic quantitative PCR (MFQPCR) system using integrated fluidic circuits (IFCs). The limit of detection of the panel was 6.25 copy/μL. Amplification-specificity was validated using several concentrations of reference materials and animal genomic DNA, leading to specific detection. In addition, target-specific detection was successfully achieved in a horse administered 20 mg of the EPO transgene via MFQPCR. Therefore, MFQPCR may be considered a suitable method for multiple-target detection in gene-doping control. To our knowledge, this is the first application of microfluidic qPCR (MFQPCR) for gene-doping control in horseracing.

Detection of non-targeted transgenes by whole-genome resequencing for gene-doping control

Gene Therapy ◽  
2020 ◽  
Author(s):  
Teruaki Tozaki ◽  
Aoi Ohnuma ◽  
Masaki Takasu ◽  
Kotono Nakamura ◽  
Mio Kikuchi ◽  
...  
Keyword(s):  
Doping Control ◽  
Whole Genome ◽  
Genome Resequencing ◽  
Gene Doping ◽  
Whole Genome Resequencing

Gene Doping – The Future of Doping?

2016 ◽  
Author(s):  
Swen Körner ◽  
Stefanie Schardien ◽  
Birte Steven-Vitense ◽  
Steffen Albach ◽  
Edgar Dorn
Keyword(s):  
Gene Doping ◽  
The Future
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