Comprehensive analysis of lncRNA and mRNA expression changes in Tibetan chicken lung tissue between three developmental stages

2020 ◽  
Vol 51 (5) ◽  
pp. 731-740
Author(s):  
Ruixiang Tang ◽  
Jiao Wang ◽  
Min Zhou ◽  
Yue Lan ◽  
Lan Jiang ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Lung Tissue ◽  
Developmental Stages ◽  
Comprehensive Analysis ◽  
Tibetan Chicken

scholarly journals Cytochrome P450-dependent biotransformation capacities in embryonic, juvenile and adult stages of zebrafish (Danio rerio)—a state-of-the-art review

2021 ◽  
Author(s):  
Ann-Kathrin Loerracher ◽  
Thomas Braunbeck
Keyword(s):  
Cytochrome P450 ◽  
Phase I ◽  
Mrna Expression ◽  
Danio Rerio ◽  
Developmental Stages ◽  
State Of The Art ◽  
Adverse Outcome Pathway ◽  
Zebrafish Embryos ◽  
Toxicological Studies ◽  
Cyp Isoforms

AbstractGiven the strong trend to implement zebrafish (Danio rerio) embryos as translational model not only in ecotoxicological, but also toxicological testing strategies, there is an increasing need for a better understanding of their capacity for xenobiotic biotransformation. With respect to the extrapolation of toxicological data from zebrafish embryos to other life stages or even other organisms, qualitative and quantitative differences in biotransformation pathways, above all in cytochrome P450-dependent (CYP) phase I biotransformation, may lead to over- or underestimation of the hazard and risk certain xenobiotic compounds may pose to later developmental stages or other species. This review provides a comprehensive state-of-the-art overview of the scientific knowledge on the development of the CYP1-4 families and corresponding phase I biotransformation and bioactivation capacities in zebrafish. A total of 68 publications dealing with spatiotemporal CYP mRNA expression patterns, activities towards mammalian CYP-probe substrates, bioactivation and detoxification activities, as well as metabolite profiling were analyzed and included in this review. The main results allow for the following conclusions: (1) Extensive work has been done to document mRNA expression of CYP isoforms from earliest embryonic stages of zebrafish, but juvenile and adult zebrafish have been largely neglected so far. (2) There is insufficient understanding of how sex- and developmental stage-related differences in expression levels of certain CYP isoforms may impact biotransformation and bioactivation capacities in the respective sexes and in different developmental stages of zebrafish. (3) Albeit qualitatively often identical, many studies revealed quantitative differences in metabolic activities of zebrafish embryos and later developmental stages. However, the actual relevance of age-related differences on the outcome of toxicological studies still needs to be clarified. (4) With respect to current remaining gaps, there is still an urgent need for further studies systematically assessing metabolic profiles and capacities of CYP isoforms in zebrafish. Given the increasing importance of Adverse Outcome Pathway (AOP) concepts, an improved understanding of CYP capacities appears essential for the interpretation and outcome of (eco)toxicological studies.

Postnatal glucocorticoids induce α-ENaC formation and regulate glucocorticoid receptors in the preterm rabbit lung

2004 ◽  
Vol 286 (1) ◽  
pp. L73-L80 ◽  
Author(s):  
Shamimunisa B. Mustafa ◽  
Robert J. DiGeronimo ◽  
Jean A. Petershack ◽  
Joseph L. Alcorn ◽  
Steven R. Seidner
Keyword(s):  
Mrna Expression ◽  
Lung Tissue ◽  
Glucocorticoid Receptors ◽  
Fold Increase ◽  
Lung Epithelial Cells ◽  
Lung Water ◽  
Protein Levels ◽  
Tissue Weight ◽  
Postnatal Treatment ◽  
Distal Lung

At birth, lung fluid clearance is coupled to Na+ transport through epithelial Na+ channels (ENaC) in the distal lung epithelium. We evaluated the effect of postnatal glucocorticoids (GC) on lung α-ENaC expression in preterm 29-day gestational age (GA) fetal rabbits. Postnatal treatment of 29-day GA fetuses with 0.5 mg/kg of dexamethasone (Dex) iv resulted in a 2- and 22-fold increase in lung α-ENaC mRNA expression compared with saline-treated fetuses after 8 and 16 h, respectively. Lung α-ENaC protein levels in Dex-treated fetuses were also elevated compared with saline-treated counterparts. The extravascular lung water (EVLW)/dry lung tissue weight ratios of 29-day GA fetuses treated with either saline or Dex decreased over 24 h compared with that observed at birth; however, at 24 h, the EVLW/dry lung tissue weight ratios of saline- and Dex-treated fetuses were similar. Dex-induced α-ENaC mRNA and protein levels were attenuated by glucocorticoid receptor (GCR) antagonist RU-486 in fetal distal lung epithelial cells isolated from 29-day GA fetuses, indicating that GC-dependent augmentation of lung α-ENaC requires the presence of functional GCR. Lung GCR mRNA expression and protein levels were elevated in 29-day GA fetuses compared with fetuses at earlier GA. Exposure of 29-day GA fetuses to Dex for 16 h caused a 2.1-fold increase in lung GCR mRNA expression, but GCR protein levels were decreased in Dex-treated fetuses after 24 h. We conclude that postnatal treatment of preterm 29-day GA fetal rabbits with GC results in an elevation of lung α-ENaC accompanied by an autoregulation of pulmonary GCR.

Angiopoietins' (Ang-1 and Ang-2) mRNA Expression Levels in Lung Tissue of IPF Patients.

2009 ◽  
Author(s):  
A Proklou ◽  
KM Antoniou ◽  
R Lymbouridou ◽  
G Soufla ◽  
K Karagiannis ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Lung Tissue ◽  
Expression Levels ◽  
Mrna Expression Levels

Serum and sputum levels of IL-17, IL-21, TNFα and mRNA expression of IL-17 in sulfur mustard lung tissue with long term pulmonary complications (28 years after sulfur mustard exposure)

2019 ◽  
Vol 76 ◽  
pp. 105828 ◽  
Author(s):  
Ali Mohammad Mohseni Majd ◽  
Soghrat Faghihzadeh ◽  
Shahryar Pourfarzam ◽  
Marzieh Eghtedardoost ◽  
Davoud Jamali ◽  
...  
Keyword(s):  
Mrna Expression ◽  
Lung Tissue ◽  
Sulfur Mustard ◽  
Pulmonary Complications

scholarly journals mRNA expression in different developmental stages of the chicken bursa of Fabricius

2016 ◽  
Vol 95 (8) ◽  
pp. 1787-1794 ◽  
Author(s):  
Xiao-dong Liu ◽  
Fubo Zhang ◽  
Hu Shan ◽  
Shu-bai Wang ◽  
Pu-Yan Chen
Keyword(s):  
Mrna Expression ◽  
Developmental Stages ◽  
Bursa Of Fabricius

scholarly journals A quantitative RT-PCR study of the mRNA expression profile of the IGF axis during mammary gland development

2004 ◽  
Vol 33 (1) ◽  
pp. 195-207 ◽  
Author(s):  
M Boutinaud ◽  
JH Shand ◽  
MA Park ◽  
K Phillips ◽  
J Beattie ◽  
...  
Keyword(s):  
Mammary Gland ◽  
Mrna Expression ◽  
Expression Profile ◽  
Mammary Gland Development ◽  
Developmental Stages ◽  
Normal Mammary Gland ◽  
Rt Pcr ◽  
Mrna Expression Profile ◽  
Igf I ◽  
Gland Development

We have used quantitative RT-PCR to analyse the mRNA expression profile of the major components of the IGF axis in different stages of murine mammary gland development, including late pregnancy, lactation and involution. We have shown that all the genes studied, IGF-I, IGF-II, IGF receptor (IGFR) and IGF-binding protein (IGFBP)-1 to -6, were expressed in every stage, albeit at greatly differing levels and displaying unique expression profiles between developmental stages. IGF-I was always expressed at significantly higher levels than either IGF-II or IGFR. This suggests that IGF-I may be the more important IGF during mammary morphogenesis. Overall, IGFBP-3 demonstrated the highest level of expression of any of the IGFBP genes throughout all the developmental stages studied. However, within developmental stages, by far the highest level of expression of any of the IGFBPs was that of IGFBP-5 at day 2 of involution; this was almost an order of magnitude higher than any of the other IGFBP levels recorded. This corroborated our previous findings that the levels of IGFBP-5 protein are highly elevated in the involuting mammary gland, and demonstrated that this up-regulation of IGFBP-5 operates at the level of transcriptional control or message stability. Comparison of the expression profile for these different genes would strongly suggest that they are likely to have differential functions throughout mammary gland development, and also highlights potential interactions and co-regulation between different members of this axis. In addition, our results have identified some similarities and differences in the expression of IGFBPs between the mouse mammary epithelial cell line, HC11, and the normal mammary gland which are worthy of study, most notably the differential regulation of IGFBP-2 and the site of expression of IGFBP-4 and -6. Overall, this study has demonstrated the importance and complexity of the IGF axis during mammary gland development and provides a valuable resource for future research in this area.

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