Expression profiles of growth‐related genes in two Nile tilapia strains and their crossbred provide insights into introgressive breeding effects

2020 ◽  
Vol 51 (4) ◽  
pp. 611-616
Author(s):  
M. E. Herkenhoff ◽  
A. O. Ribeiro ◽  
J. M. Costa ◽  
A. C. Oliveira ◽  
M. A. D. Dias ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Expression Profiles ◽  
Introgressive Breeding

scholarly journals Genome-Wide Identification, Evolution and Expression of the Complete Set of Cytoplasmic Ribosomal Protein Genes in Nile Tilapia

2020 ◽  
Vol 21 (4) ◽  
pp. 1230
Author(s):  
Gangqiao Kuang ◽  
Wenjing Tao ◽  
Shuqing Zheng ◽  
Xiaoshuang Wang ◽  
Deshou Wang
Keyword(s):  
Ribosomal Proteins ◽  
Nile Tilapia ◽  
Ribosome Biogenesis ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Ribosomal Protein Genes ◽  
Expression Levels ◽  
Sexually Dimorphic Expression ◽  
Complete Set ◽  
Almost All

Ribosomal proteins (RPs) are indispensable in ribosome biogenesis and protein synthesis, and play a crucial role in diverse developmental processes. In the present study, we carried out a comprehensive analysis of RPs in chordates and examined the expression profiles of the complete set of 92 cytoplasmic RP genes in Nile tilapia. The RP genes were randomly distributed throughout the tilapia genome. Phylogenetic and syntenic analyses revealed the existence of duplicated RP genes from 2R (RPL3, RPL7, RPL22 and RPS27) and 3R (RPL5, RPL19, RPL22, RPL41, RPLP2, RPS17, RPS19 and RPS27) in tilapia and even more from 4R in common carp and Atlantic salmon. The RP genes were found to be expressed in all tissues examined, but their expression levels differed among different tissues. Gonadal transcriptome analysis revealed that almost all RP genes were highly expressed, and their expression levels were highly variable between ovaries and testes at different developmental stages in tilapia. No sex- and stage-specific RP genes were found. Eleven RP genes displayed sexually dimorphic expression with nine higher in XY gonad and two higher in XX gonad at all stages examined, which were proved to be phenotypic sex dependent. Quantitative real-time PCR and immunohistochemistry ofRPL5b and RPL24 were performed to validate the transcriptome data. The genomic resources and expression data obtained in this study will contribute to a better understanding of RPs evolution and functions in chordates.

Identification and expression profiles of multiple genes in Nile tilapia in response to bacterial infections

2011 ◽  
Vol 144 (1-2) ◽  
pp. 111-119 ◽  
Author(s):  
Julia W. Pridgeon ◽  
Mediha Aksoy ◽  
Phillip H. Klesius ◽  
Yuehong Li ◽  
Xingjiang Mu ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Bacterial Infections ◽  
Expression Profiles ◽  
Multiple Genes

scholarly journals Transdifferentiation of Differentiated Ovary into Functional Testis by Long-Term Treatment of Aromatase Inhibitor in Nile Tilapia

Endocrinology ◽  
2014 ◽  
Vol 155 (4) ◽  
pp. 1476-1488 ◽  
Author(s):  
Li-Na Sun ◽  
Xiao-Long Jiang ◽  
Qing-Ping Xie ◽  
Jing Yuan ◽  
Bao-Feng Huang ◽  
...  
Keyword(s):  
Aromatase Inhibitor ◽  
Sertoli Cells ◽  
Nile Tilapia ◽  
Expression Profiles ◽  
Sex Reversal ◽  
Term Treatment ◽  
Germline Stem Cell ◽  
Long Term Treatment ◽  
17Β Estradiol

Females with differentiated ovary of a gonochoristic fish, Nile tilapia, were masculinized by long-term treatment with an aromatase inhibitor (Fadrozole) in the present study. The reversed gonads developed into functional testes with fertile sperm. The longer the fish experienced sex differentiation, the longer treatment time was needed for successful sex reversal. Furthermore, Fadrozole-induced sex reversal, designated as secondary sex reversal (SSR), was successfully rescued by supplement of exogenous 17β-estradiol. Gonadal histology, immunohistochemistry, transcriptome, and serum steroid level were analyzed during SSR. The results indicated that spermatogonia were transformed from oogonia or germline stem cell-like cells distributed in germinal epithelium, whereas Leydig and Sertoli cells probably came from the interstitial cells and granulosa cells of the ovarian tissue, respectively. The transdifferentiation of somatic cells, as indicated by the appearance of doublesex- and Mab-3-related transcription factor 1 (pre-Sertoli cells) and cytochrome P450, family 11, subfamily B, polypeptide 2 (pre-Leydig cells)-positive cells in the ovary, provided microniche for the transdifferentiation of germ cells. Decrease of serum 17β-estradiol was detected earlier than increase of serum 11-ketotestosterone, indicating that decrease of estrogen was the cause, whereas increase of androgen was the consequence of SSR. The sex-reversed gonad displayed more similarity in morphology and histology with a testis, whereas the global gene expression profiles remained closer to the female control. Detailed analysis indicated that transdifferentiation was driven by suppression of female pathway genes and activation of male pathway genes. In short, SSR provides a good model for study of sex reversal in teleosts and for understanding of sex determination and differentiation in nonmammalian vertebrates.

scholarly journals miRTil: An Extensive Repository for Nile Tilapia microRNA Next Generation Sequencing Data

Cells ◽  
2020 ◽  
Vol 9 (8) ◽  
pp. 1752
Author(s):  
Luiz Augusto Bovolenta ◽  
Danillo Pinhal ◽  
Marcio Luis Acencio ◽  
Arthur Casulli de Oliveira ◽  
Simon Moxon ◽  
...  
Keyword(s):  
Nile Tilapia ◽  
Developmental Stages ◽  
Expression Profiles ◽  
Interaction Network ◽  
Mature Mirnas ◽  
Next Generation Sequencing Data ◽  
Sequencing Data ◽  
Genomic Context ◽  
Protein Coding ◽  
Post Transcriptional Regulation

Nile tilapia is the third most cultivated fish worldwide and a novel model species for evolutionary studies. Aiming to improve productivity and contribute to the selection of traits of economic impact, biotechnological approaches have been intensively applied to species enhancement. In this sense, recent studies have focused on the multiple roles played by microRNAs (miRNAs) in the post-transcriptional regulation of protein-coding genes involved in the emergence of phenotypes with relevance for aquaculture. However, there is still a growing demand for a reference resource dedicated to integrating Nile Tilapia miRNA information, obtained from both experimental and in silico approaches, and facilitating the analysis and interpretation of RNA sequencing data. Here, we present an open repository dedicated to Nile Tilapia miRNAs: the “miRTil database”. The database stores data on 734 mature miRNAs identified in 11 distinct tissues and five key developmental stages. The database provides detailed information about miRNA structure, genomic context, predicted targets, expression profiles, and relative 5p/3p arm usage. Additionally, miRTil also includes a comprehensive pre-computed miRNA-target interaction network containing 4936 targets and 19,580 interactions.

1327: Gene Expression Profiles in Benign Prostatic Hyperplasia

2004 ◽  
Vol 171 (4S) ◽  
pp. 349-350
Author(s):  
Gaelle Fromont ◽  
Michel Vidaud ◽  
Alain Latil ◽  
Guy Vallancien ◽  
Pierre Validire ◽  
...  
Keyword(s):  
Gene Expression ◽  
Benign Prostatic Hyperplasia ◽  
Expression Profiles ◽  
Gene Expression Profiles ◽  
Prostatic Hyperplasia
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