Ruminal transcript abundance of the centromere‐associated protein E gene may influence residual feed intake in beef steers

2020 ◽  
Vol 51 (3) ◽  
pp. 453-456
Author(s):  
A. R. Rathert ◽  
A. M. Meyer ◽  
A. P. Foote ◽  
R. J. Kern ◽  
H. C. Cunningham‐Hollinger ◽  
...  
2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 401-401
Author(s):  
E Andreini ◽  
S Augenstein ◽  
C Fales ◽  
R Sainz ◽  
J Oltjen

2014 ◽  
Vol 45 (4) ◽  
pp. 461-472 ◽  
Author(s):  
A. K. Lindholm-Perry ◽  
L. A. Kuehn ◽  
W. T. Oliver ◽  
R. J. Kern ◽  
R. A. Cushman ◽  
...  

2012 ◽  
Vol 43 (5) ◽  
pp. 599-603 ◽  
Author(s):  
A. K. Lindholm-Perry ◽  
L. A. Kuehn ◽  
W. M. Snelling ◽  
T. P. L. Smith ◽  
C. L. Ferrell ◽  
...  

2019 ◽  
Vol 97 (Supplement_3) ◽  
pp. 217-217
Author(s):  
Sheyenne M Augenstein ◽  
Emily M Andreini ◽  
James W Oltjen ◽  
Roberto Sainz

Abstract Activity level may affect residual feed intake (RFI), and cattle that move more may have less energy for gain. We compared activity level in high and low RFI beef steers under different nutritional conditions. Lying time, lying duration and lying bouts were recorded at 1-minute intervals utilizing Hobo G Pendant Loggers attached to the right hind leg. To identify RFI classification, 57 Angus-cross steers were individually housed and offered ad libitum access to a TMR for 56 d. RFI was defined as the residual of the regression of DMI on mid-test BW0.75 and ADG. High and low RFI groups (14 steers each) were defined as > 0.5 SD above or below the mean of zero, respectively. Ad libitum feeding was followed by restricted feeding at 75% of previous ad libitum DMI/BW0.75. After 56 d ad libitum feeding, steers were moved to group pens, ad libitum fed 4 days (ADLIBFED), then fasted 4 days (ADLIBFASTED). This was followed by the 56 d individual restricted feeding, after which, steers were moved to group pens, fed 4 days of restricted DMI (RESTRICTFED), then fasted 4 days (RESTRICTFASTED). There was no difference in lying time (800 and 804 minutes/day, P = 0.85), lying bouts per day (9.8 and 11.4, P = 0.16) and lying duration (99 and 84 minutes/bout, P = 0.18) between low RFI and high RFI cattle, respectively. Fasted animals had less lying time each day (P < 0.05), manifested by fewer lying bouts per day (P < 0.05), that tended (P > 0.05) to be slightly longer than fed animals. Ad lib animals tended (P > 0.05) to have less lying time each day, with a similar number of lying bouts per day, that were slightly (P > 0.05) shorter than restricted animals. Although activity is not affected by RFI level, results suggest that periods of limited feed availability increases activity level.


2018 ◽  
Vol 96 (suppl_3) ◽  
pp. 401-401
Author(s):  
N Ferriman ◽  
C Campbell ◽  
K Wood ◽  
A Edwards ◽  
I Mandell

2019 ◽  
Vol 97 (5) ◽  
pp. 2181-2187
Author(s):  
Ahmed A Elolimy ◽  
Emad Abdel-Hamied ◽  
Liangyu Hu ◽  
Joshua C McCann ◽  
Daniel W Shike ◽  
...  

Abstract Residual feed intake (RFI) is a widely used measure of feed efficiency in cattle. Although the precise biologic mechanisms associated with improved feed efficiency are not well-known, most-efficient steers (i.e., with low RFI coefficient) downregulate abundance of proteins controlling protein degradation in skeletal muscle. Whether cellular mechanisms controlling protein turnover in ruminal tissue differ by RFI classification is unknown. The aim was to investigate associations between RFI and signaling through the mechanistic target of rapamycin (MTOR) and ubiquitin-proteasome pathways in ruminal epithelium. One hundred and forty-nine Red Angus cattle were allocated to 3 contemporary groups according to sex and herd origin. Animals were offered a finishing diet for 70 d to calculate the RFI coefficient for each. Within each group, the 2 most-efficient (n = 6) and least-efficient animals (n = 6) were selected. Compared with least-efficient animals, the most-efficient animals consumed less feed (P < 0.05; 18.36 vs. 23.39 kg/d DMI). At day 70, plasma samples were collected for insulin concentration analysis. Ruminal epithelium was collected immediately after slaughter to determine abundance and phosphorylation status of 29 proteins associated with MTOR, ubiquitin-proteasome, insulin signaling, and glucose and amino acid transport. Among the proteins involved in cellular protein synthesis, most-efficient animals had lower (P ≤ 0.05) abundance of MTOR, p-MTOR, RPS6KB1, EIF2A, EEF2K, AKT1, and RPS6KB1, whereas MAPK3 tended (P = 0.07) to be lower. In contrast, abundance of p-EEF2K, p-EEF2K:EEF2K, and p-EIF2A:EIF2A in most-efficient animals was greater (P ≤ 0.05). Among proteins catalyzing steps required for protein degradation, the abundance of UBA1, NEDD4, and STUB1 was lower (P ≤ 0.05) and MDM2 tended (P = 0.06) to be lower in most-efficient cattle. Plasma insulin and ruminal epithelium insulin signaling proteins did not differ (P > 0.05) between RFI groups. However, abundance of the insulin-responsive glucose transporter SLC2A4 and the amino acid transporters SLC1A3 and SLC1A5 also was lower (P ≤ 0.05) in most-efficient cattle. Overall, the data indicate that differences in signaling mechanisms controlling protein turnover and nutrient transport in ruminal epithelium are components of feed efficiency in beef cattle.


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