Identification of genes in longissimus dorsi muscle differentially expressed between Wannanhua and Yorkshire pigs using RNA-sequencing

2016 ◽  
Vol 47 (3) ◽  
pp. 324-333 ◽  
Author(s):  
X.-J. Li ◽  
J. Zhou ◽  
L.-Q. Liu ◽  
K. Qian ◽  
C.-L. Wang
Keyword(s):  
Rna Sequencing ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Yorkshire Pigs

Isolation, identification and expression profile of fourteen differentially expressed sequence tags in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs

2007 ◽  
Vol 4 (1) ◽  
pp. 9-14
Author(s):  
Liu Yong-Gang ◽  
Xiong Yuan-Zhu ◽  
Zuo Bo ◽  
Jiang Si-Wen ◽  
Deng Chang-Yan ◽  
...  
Keyword(s):  
Expression Profile ◽  
Differential Display ◽  
Expressed Sequence Tags ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Large White ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Muscle Tissues ◽  
Expressed Sequence

AbstractIn order to detect the molecular mechanism of heterosis in pigs, an mRNA differential display (DD) technique was performed to investigate the differences in gene expression in the longissimus dorsi muscle tissues from Meishan, Meishan×Large White cross and Large White pigs. Fourteen expressed sequence tags (ESTs), differentially expressed between the hybrid and purebred pigs, were isolated and identified through semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). Nucleotide sequence analysis revealed that the 14 ESTs are not homologous to any of the known genes or ESTs. These novel ESTs were then deposited in the GenBank database. Tissue expression profile analysis showed that the ESTs were expressed in most tissues, including heart, spleen, liver, kidney, small intestine, ovary and lung, and this also implied that these genes must be important for the life process. Our results indicate the diversity of differential display of genes between the hybrids and purebreds in the Meishan×Large White cross combination. Results also suggest that heterosis in pigs might be derived from the differential expression of many indispensable genes in specific life phases.

scholarly journals Competitive endogenous RNA network reveals that miR-1343 play key regulatory roles in longissimus dorsi muscle between Saba and Yorkshire pigs

2019 ◽  
Author(s):  
Wei Hu ◽  
Qiang Chen ◽  
Xiaoyi Wang ◽  
Xinxing Dong ◽  
Mingli Li ◽  
...  
Keyword(s):  
Regulatory Mechanism ◽  
Longissimus Dorsi ◽  
Pork Quality ◽  
Regulation Mechanism ◽  
Pig Breeds ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Muscle Formation ◽  
Yorkshire Pigs ◽  
Quality Formation

Abstract Background Pork quality is an important economic trait in pig industry, and increasing data shows that Chinese indigenous and Western commercial pig breeds have significant differences in pork quality. However, the molecular regulatory mechanism of pork quality formation is still unclear. The aim of this study was to explore the transcriptional regulation mechanism of pork quality formation of Chinese Saba pig (SS) and Western Yorkshire pig (YY) breeds. Results A total of 31 DEmiRNAs, 902 DEmRNAs, 47 DElncRNAs and 198 DEcircRNAs were identified in longissimus dorsi muscles between SSs and YYs. On the basis of differentially expressed RNAs, two ceRNA networks including lncRNA-miRNA-mRNA and circRNA-miRNA-mRNA were established, and two ceRNA networks showed that miR-1343 had the most links in two ceRNA networks, which demonstrated that miR-1343 might play key regulatory roles in longissimus dorsi muscle formation between SS and YY breeds. Conclusions The present study identified miR-1343 as key regulatory factor in pig longissimus dorsi muscle formation by ceRNA networks, which provides novel insights into molecular regulatory mechanism of pork quality formation between fat-type and lean-type pig breeds.

scholarly journals Isolation, Sequence Analysis and Expression Profile of a Novel Swine Gene Differentially Expressed in the Longissimus Dorsi Muscle Tissues from Landrace×Large White Cross-combination

2005 ◽  
Vol 37 (3) ◽  
pp. 186-191 ◽  
Author(s):  
Yong-Gang Liu ◽  
Yuan-Zhu Xiong ◽  
Chang-Yan Deng
Keyword(s):  
Carbonic Anhydrase ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Cross Combination ◽  
Large White ◽  
Phylogenetic Tree Analysis ◽  
Longissimus Dorsi Muscle ◽  
Carbonic Anhydrase Iii ◽  
Dorsi Muscle ◽  
Muscle Tissues

Abstract The mRNA differential display technique was performed to investigate the differences in gene expression in the Longissimus dorsi muscle tissues from Landrace×Large White cross-combination. One novel gene that was differentially expressed was identified using semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) and its complete cDNA sequence was obtained using the rapid amplification of cDNA ends (RACE) method. The nucleotide sequence of the gene is not homologous to any of the known porcine genes. The sequence prediction analysis revealed that the open reading frame of this gene encodes a protein of 260 amino acids that contains the putative conserved domain of the carbonic anhydrase, and this protein has high homology with the carbonic anhydrase III (CA-III) of four species-mouse (91%), horse (91%), rat (89%) and human (86%)–so that it can be defined as swine carbonic anhydrase III. The phylogenetic tree analysis revealed that the swine CA-III has a closer genetic relationship with the horse CA-III than with those of mouse, rat and human. The tissue expression analysis indicated that the swine CA-III gene is generally expressed in most tissues. Our experiment is the first to establish the primary foundation for further research on the swine CA-III gene.

0903 Differentially expressed genes in genetically divergent Nellore steers for calcium content in the Longissimus dorsi muscle

2016 ◽  
Vol 94 (suppl_5) ◽  
pp. 435-435 ◽  
Author(s):  
J. Afonso ◽  
P. C. Tizioto ◽  
P. S. N. Oliveira ◽  
W. J. S. Diniz ◽  
A. O. D. Lima ◽  
...  
Keyword(s):  
Differentially Expressed Genes ◽  
Calcium Content ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle

scholarly journals Comprehensive Analysis of Differentially Expressed mRNA, lncRNA and circRNA and Their ceRNA Networks in the Longissimus Dorsi Muscle of Two Different Pig Breeds

2019 ◽  
Vol 20 (5) ◽  
pp. 1107 ◽  
Author(s):  
Jing Wang ◽  
Qiaoling Ren ◽  
Liushuai Hua ◽  
Junfeng Chen ◽  
Jiaqing Zhang ◽  
...  
Keyword(s):  
Regulatory Networks ◽  
Myogenic Differentiation ◽  
Wnt Signaling Pathway ◽  
Circular Rna ◽  
Differentially Expressed ◽  
Longissimus Dorsi ◽  
Longissimus Dorsi Muscle ◽  
Dorsi Muscle ◽  
Non Coding Rna ◽  
Differentially Expressed Mrna

Circular RNA (circRNA) and long non-coding RNA (lncRNA) are known to participate in adipogenesis and myogenic differentiation, but their impact on porcine muscle traits is not well understood. We compared their expressional profiles in the longissimus dorsi muscle of Chinese Huainan pigs (HN, the fat type) and Western commercial Duroc×(Landrace×Yorkshire) (DLY, the thin type) pigs, and 854 mRNAs, 233 lncRNAs, and 66 circRNAs (p < 0.05 and ｜log2FoldChange｜>1) were found to be differentially expressed. The differentially expressed mRNA and circRNA parental genes were enriched in the Wnt signaling pathway (adipogenesis), the transition between fast and slow fibers (myogenic differentiation), and alanine, aspartate and glutamate metabolism (pork flavor). The potential lncRNAs/circRNAs-miRNAs-mRNAs regulatory networks shared MYOD1, PPARD, miR-423-5p and miR-874, which were associated with skeletal muscle muscular proliferation, differentiation/regeneration and adipogenesis. Taken together, these differentially expressed non-coding RNAs may be involved in the molecular basis of muscle traits, acting as the competing endogenous RNA (ceRNA) for miRNAs.

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