Demography of a genetic sexing strain of Anastrepha ludens (Diptera: Tephritidae): effects of selection based on mating performance

2017 ◽  
Vol 20 (1) ◽  
pp. 1-8 ◽  
Author(s):  
Luis Quintero-Fong ◽  
Jorge Toledo ◽  
Lorena Ruiz-Montoya ◽  
Pedro Rendón ◽  
Dina Orozco-Dávila ◽  
...  
Keyword(s):  
Anastrepha Ludens ◽  
Genetic Sexing ◽  
Genetic Sexing Strain ◽  
Mating Performance

Female pupae of the genetic sexing strain “Tap-7” of Anastrepha ludens as hosts of Coptera haywardi

BioControl ◽  
2013 ◽  
Vol 59 (2) ◽  
pp. 149-157 ◽  
Author(s):  
Florida López-Arriaga ◽  
Pablo Montoya ◽  
Jorge Cancino ◽  
Jorge Toledo ◽  
Pablo Liedo
Keyword(s):  
Anastrepha Ludens ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Pre- and Post-Copulatory Competitiveness of the Genetic Sexing Strain Tapachula-7 of Anastrepha ludens (Diptera: Tephritidae)

2020 ◽  
Vol 113 (5) ◽  
pp. 2163-2170
Author(s):  
Yair Contreras-Navarro ◽  
Diana Pérez-Staples ◽  
Dina Orozco-Dávila ◽  
Francisco Díaz-Fleischer
Keyword(s):  
Sterile Insect Technique ◽  
Copulatory Behavior ◽  
Mass Rearing ◽  
Economic Importance ◽  
Anastrepha Ludens ◽  
Sexual Performance ◽  
Field Cage ◽  
Wild Female ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Abstract Anastrepha ludens (Loew) (Diptera: Tephritidae) is an endemic pest of Mexico, attacking several fruits of economic importance. The Sterile Insect Technique (SIT), consisting of mass-rearing, irradiation and release of adults, is used to control this pest in affected areas. Currently, a genetic sexing strain (Tapachula 7, Tap-7) consisting only of A. ludens males is being released, yet we lack information on its sexual performance at the early ages when they are released and on its post copulatory behavior in terms of sperm transfer and mating inhibition. Here, sexual competitiveness at young ages and ability to inhibit female re-mating was compared between sterile Tap-7, Standard Bisexual (SB), or wild males both in laboratory and field cage conditions. Sperm stored by females mating with wild, Tap-7, or SB strain males was also compared. Six-day-old sterile Tap-7 males had low mating rates, however, by 7 d of age 80% of males had mated. Tap-7 males were just as likely as wild males to inhibit wild female re-mating. In field cages, sterile Tap-7 males mated faster than wild and sterile males from the SB strain and had comparable mating success to wild males. Females mated to sterile Tap-7 males stored more sperm than those mated to sterile SB males. Females mated to wild males stored more sperm than females mated to any other type of male. Mass-rearing had a greater effect on decreasing sperm stored by females than irradiation. We recommend continuing the release of the GSS strain of A. ludens.

scholarly journals A Novel Genetic Sexing Strain of Anastrepha Ludens for Cost-Effective Sterile Insect Technique Applications: Improved Genetic Stability and Rearing Efficiency

Insects ◽  
2021 ◽  
Vol 12 (6) ◽  
pp. 499
Author(s):  
Edwin Ramírez-Santos ◽  
Pedro Rendon ◽  
Georgia Gouvi ◽  
Antigone Zacharopoulou ◽  
Kostas Bourtzis ◽  
...  
Keyword(s):  
Genetic Stability ◽  
Sterile Insect Technique ◽  
Production Efficiency ◽  
Small Scale ◽  
Management Approach ◽  
Anastrepha Ludens ◽  
Male Mating ◽  
Pest Species ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Anastrepha ludens (Loew) is one of the most destructive insect pests damaging several fruits of economic importance. The sterile insect technique (SIT) is used under an area-wide integrated pest management approach, to suppress these pest populations. Mass rearing facilities were initially established to produce sterile males of bi-sexual strains in support of SIT. The first genetic sexing strain (GSS) for A. ludens, Tapachula-7, based on pupal color dimorphism, was a key development since the release of males-only significantly increases the SIT efficiency. In this study, we document the development of a novel pupal color-based GSS. Twelve radiation-induced translocation lines were assessed as potential GSS in terms of recombination rates and rearing efficiency at a small scale. The best one, GUA10, was cytogenetically characterized: it was shown to carry a single translocation between the Y chromosome and chromosome 2, which is known to carry the black pupae marker. This GSS was further evaluated at medium and large scales regarding its genetic stability, productivity and quality versus Tapachula-7. GUA10 presented better genetic stability, fecundity, fertility, production efficiency, flying ability, and male mating, clear indicators that GUA10 GSS can significantly improve the efficacy and cost-effectiveness of SIT applications against this pest species.

Performance of the Tap-7 Genetic Sexing Strain Used to Control Anastrepha ludens Populations in the Citrus Region of Tamaulipas, Mexico

2019 ◽  
pp. 177-189
Author(s):  
Salvador Flores ◽  
Sergio Campos ◽  
Enoc Gómez ◽  
Rubén Leal Mubarqui ◽  
Jorge Luis Morales-Marin ◽  
...  
Keyword(s):  
Anastrepha Ludens ◽  
Genetic Sexing ◽  
Genetic Sexing Strain

Selection by mating competitiveness improves the performance of Anastrepha ludens males of the genetic sexing strain Tapachula-7

2016 ◽  
Vol 106 (5) ◽  
pp. 624-632 ◽  
Author(s):  
L. Quintero-Fong ◽  
J. Toledo ◽  
L. Ruiz ◽  
P. Rendón ◽  
D. Orozco-Dávila ◽  
...  
Keyword(s):  
Development Time ◽  
Mass Rearing ◽  
Quality Parameters ◽  
Anastrepha Ludens ◽  
Sexual Performance ◽  
Genetic Sexing ◽  
Mating Competitiveness ◽  
Colony Management ◽  
Management Method ◽  
Genetic Sexing Strain

AbstractThe sexual performance of Anastrepha ludens males of the Tapachula-7 genetic sexing strain, produced via selection based on mating success, was compared with that of males produced without selection in competition with wild males. Mating competition, development time, survival, mass-rearing quality parameters and pheromone production were compared. The results showed that selection based on mating competitiveness significantly improved the sexual performance of offspring. Development time, survival of larvae, pupae and adults, and weights of larvae and pupae increased with each selection cycle. Differences in the relative quantity of the pheromone compounds (Z)-3-nonenol and anastrephin were observed when comparing the parental males with the F4 and wild males. The implications of this colony management method on the sterile insect technique are discussed.

scholarly journals Determining the Sterilization Doses under Hypoxia for the Novel Black Pupae Genetic Sexing Strain of Anastrepha fraterculus (Diptera, Tephritidae)

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 308
Author(s):  
Paloma Della Giustina ◽  
Thiago Mastrangelo ◽  
Sohel Ahmad ◽  
Gabriel Mascarin ◽  
Carlos Caceres
Keyword(s):  
Fruit Fly ◽  
South American ◽  
Response Curves ◽  
Genetic Sexing ◽  
Egg Hatch ◽  
Anastrepha Fraterculus ◽  
Common Strategy ◽  
Genetic Sexing Strain ◽  
South American Fruit Fly ◽  
First Time

A common strategy used to maintain sterile fly quality without sacrificing sterility is to irradiate the insects under an oxygen-reduced atmosphere. So far, sterilizing doses for the South American fruit fly Anastrepha fraterculus have only been determined under normoxia. Our study reports for the first time the dose-sterility response under hypoxia for two different A. fraterculus strains. The pupae were derived from a bisexual strain (a Brazilian-1 population) and a recently developed genetic sexing strain (GSS-89). Two hours prior to irradiation, pupae were transferred to sealed glass bottles and irradiated when oxygen concentration was below 3%. Four types of crosses with nonirradiated flies of the bisexual strain were set to assess sterility for each radiation dose. For males from both strains, Weibull dose–response curves between radiation doses and the proportion of egg hatch, egg-to-pupa recovery, and recovery of adults were determined. The GSS males revealed high sterility/mortality levels compared to males from the bisexual strain at doses < 40 Gy, but a dose of 74 Gy reduced egg hatch by 99% regardless of the male strain and was considered the sterilizing dose. The fertility of irradiated females was severely affected even at low doses under hypoxia.

scholarly journals Pupation Substrate Type and Volume Affect Pupation, Quality Parameters and Production Costs of a Reproductive Colony of Ceratitis capitata (Diptera: Tephritidae) VIENNA 8 Genetic Sexing Strain

Insects ◽  
2021 ◽  
Vol 12 (4) ◽  
pp. 337
Author(s):  
Carlos Pascacio-Villafán ◽  
Luis Quintero-Fong ◽  
Larissa Guillén ◽  
José Pedro Rivera-Ciprian ◽  
Reynaldo Aguilar ◽  
...  
Keyword(s):  
Wheat Bran ◽  
Ceratitis Capitata ◽  
Mass Rearing ◽  
Physicochemical Characteristics ◽  
Production Costs ◽  
Cellulose I ◽  
Coconut Fiber ◽  
Genetic Sexing ◽  
Recycled Paper ◽  
Genetic Sexing Strain

Adequate pupation substrates and substrate volume are critical factors in the mass-rearing of insects for Sterile Insect Technique (SIT) applications. To identify an ideal pupation substrate for a reproductive colony of Ceratitis capitata (Wiedemann) VIENNA 8 genetic sexing strain, we first examined pupation in cellulose from recycled paper (cellulose I), sawdust, fine wheat bran, vermiculite and coconut fiber using a volume of 2.5–12.5 mL of substrate for each 5 mL volume of fly larvae. We found a positive relationship between substrate volume and pupation, with cellulose I generating the highest proportions of pupation and coconut fiber the lowest. Higher proportions of female flies (white pupae) pupated in sawdust. The proportion of female fliers increased as substrate volume rose in sawdust and coconut fiber, whereas it decreased in vermiculite and cellulose. In a second experiment, we tested three types of cellulose differing in physicochemical characteristics (celluloses I, II and III), sawdust, and fine wheat bran using a substrate:larvae ratio of 1:1. The three types of cellulose produced the highest pupation levels. The highest proportions of female fliers were observed in sawdust, and cellulose types III and II. Cellulose III and sawdust at relatively low volumes were more cost-effective to produce one million pupae than other substrates, including fine wheat bran used in a mass-rearing facility in Mexico.

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