Development and use of a single real‐time PCR assay to identify the three spider mite species Tetranychus urticae , Tetranychus lambi and Tetranychus ludeni (Acari: Tetranychidae)

2020 ◽  
Vol 59 (3) ◽  
pp. 630-638
Author(s):  
Yizhou Chen ◽  
Lauren Woolley ◽  
Duong Nguyen ◽  
Risha Gupta ◽  
Gregory T Chandler ◽  
...  
Keyword(s):  
Real Time ◽  
Tetranychus Urticae ◽  
Real Time Pcr ◽  
Spider Mite ◽  
Mite Species ◽  
Pcr Assay ◽  
Tetranychus Ludeni

scholarly journals Development and Validation of a Real-Time PCR Assay for Rapid Detection of Two-Spotted Spider Mite, Tetranychus urticae (Acari: Tetranychidae)

PLoS ONE ◽  
2015 ◽  
Vol 10 (7) ◽  
pp. e0131887 ◽  
Author(s):  
Dongmei Li ◽  
Qing-Hai Fan ◽  
David W. Waite ◽  
Disna Gunawardana ◽  
Sherly George ◽  
...  
Keyword(s):  
Real Time ◽  
Tetranychus Urticae ◽  
Real Time Pcr ◽  
Rapid Detection ◽  
Spider Mite ◽  
Pcr Assay ◽  
Two Spotted Spider Mite ◽  
Development And Validation

scholarly journals A misleading false-negative result of Pneumocystis real-time PCR assay due to a rare punctual mutation: A French multicenter study

2016 ◽  
Vol 55 (2) ◽  
pp. 180-184 ◽  
Author(s):  
Solène Le Gal ◽  
Florence Robert-Gangneux ◽  
Yann Pépino ◽  
Sorya Belaz ◽  
Céline Damiani ◽  
...  
Keyword(s):  
Real Time ◽  
Multicenter Study ◽  
Real Time Pcr ◽  
False Negative ◽  
False Negative Result ◽  
Pcr Assay

scholarly journals Differing Effects of Standard and Harsh Nucleic Acid Extraction Procedures on Diagnostic Helminth Real-Time PCRs Applied to Human Stool Samples

Pathogens ◽  
2021 ◽  
Vol 10 (2) ◽  
pp. 188
Author(s):  
Tanja Hoffmann ◽  
Andreas Hahn ◽  
Jaco J. Verweij ◽  
Gérard Leboulle ◽  
Olfert Landt ◽  
...  
Keyword(s):  
Nucleic Acid ◽  
Real Time ◽  
Real Time Pcr ◽  
Acid Extraction ◽  
Nucleic Acid Extraction ◽  
Pcr Assay ◽  
Bead Beating ◽  
Stool Samples ◽  
Pcr Assays ◽  
Human Stool

This study aimed to assess standard and harsher nucleic acid extraction schemes for diagnostic helminth real-time PCR approaches from stool samples. A standard procedure for nucleic acid extraction from stool and a procedure including bead-beating as well as proteinase K digestion were compared with group-, genus-, and species-specific real-time PCR assays targeting helminths and nonhelminth pathogens in human stool samples. From 25 different in-house and commercial helminth real-time PCR assays applied to 77 stool samples comprising 67 historic samples and 10 external quality assessment scheme samples positively tested for helminths, higher numbers of positive test results were observed after bead-beating-based nucleic acid extraction for 5/25 (20%) real-time PCR assays irrespective of specificity issues. Lower cycle threshold values were observed for one real-time PCR assay after the standard extraction scheme, and for four assays after the bead-beating-based scheme. Agreement between real-time PCR results after both nucleic acid extraction strategies according to Cohen’s kappa ranged from poor to almost perfect for the different assays. Varying agreement was observed in eight nonhelminth real-time PCR assays applied to 67 historic stool samples. The study indicates highly variable effects of harsh nucleic acid extraction approaches depending on the real-time PCR assay used.

Development and application of a TaqMan-based real-time PCR assay for specifically detecting feline astrovirus

2021 ◽  
pp. 101729
Author(s):  
Guoying Dong ◽  
Qian Wang ◽  
Jiangting Niu ◽  
Yiqin Cai ◽  
Yanbing Guo ◽  
...  
Keyword(s):  
Real Time ◽  
Real Time Pcr ◽  
Pcr Assay
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