Morphological versus molecular identification ofCulex annulirostris Skuse andCulex palpalis Taylor: key members of theCulex sitiens(Diptera: Culicidae) subgroup in Australasia

2013 ◽  
Vol 52 (4) ◽  
pp. 356-362 ◽  
Author(s):  
Cassie C Jansen ◽  
Stéphane Hemmerter ◽  
Andrew F van den Hurk ◽  
Peter I Whelan ◽  
Nigel W Beebe
Keyword(s):  
Molecular Identification

Electron microscopy in diagnostic virology

1984 ◽  
Vol 42 ◽  
pp. 70-73
Author(s):  
S. E. Miller
Keyword(s):  
Electron Microscopy ◽  
Tissue Culture ◽  
Molecular Identification ◽  
Point Of View ◽  
Negative Stain ◽  
Viral Diagnosis ◽  
Electron Microscopist ◽  
Diagnostic Virology

The techniques for detecting viruses are many and varied including FAT, ELISA, SPIRA, RPHA, SRH, TIA, ID, IEOP, GC (1); CF, CIE (2); Tzanck (3); EM, IEM (4); and molecular identification (5). This paper will deal with viral diagnosis by electron microscopy and will be organized from the point of view of the electron microscopist who is asked to look for an unknown agent--a consideration of the specimen and possible agents rather than from a virologist's view of comparing all the different viruses. The first step is to ascertain the specimen source and select the method of preparation, e. g. negative stain or embedment, and whether the sample should be precleared by centrifugation, concentrated, or inoculated into tissue culture. Also, knowing the type of specimen and patient symptoms will lend suggestions of possible agents and eliminate some viruses, e. g. Rotavirus will not be seen in brain, nor Rabies in stool, but preconceived notions should not prejudice the observer into missing an unlikely pathogen.

Molecular Identification of Stranded Cetaceans in Coastal China

2019 ◽  
Vol 45 (5) ◽  
pp. 525-532
Author(s):  
Ting Li ◽  
Hao Wu ◽  
Caiwen Wu ◽  
Guang Yang ◽  
Bingyao Chen
Keyword(s):  
Molecular Identification ◽  
Coastal China

Colonization of decomposing Sphagnum moss litter by mycorrhizal roots in two types of peatland ecosystems

2014 ◽  
Vol 10 ◽  
pp. 113-121
Author(s):  
Mateusz Wilk ◽  
Julia Pawłowska ◽  
Marta Wrzosek ◽  
Michał Gorczak ◽  
Małgorzata Suska-Malawska
Keyword(s):  
Molecular Identification ◽  
Decomposition Process ◽  
Sphagnum Moss ◽  
Forest Communities ◽  
Litter Bags ◽  
Poor Fen ◽  
The Poor ◽  
Ectomycorrhizal Roots ◽  
Mycorrhizal Roots ◽  
Ectomycorrhizal Morphotypes

During a 35-month study on the decomposition of Sphagnum moss litter in poor fen and pine bog forest, an intensive colonization of litter-bags by mycorrhizal roots was observed during the decomposition process. Content of mycorrhizal roots in litter-bags, expressed as % mass of roots, was generally increasing during the decomposition in pine bog forest, and fluctuating during decomposition on poor fen, although in both cases the results were statistically insignificant. Two morphotypes of ericoid roots and two morphotypes of ectomycorrhizal roots were recorded from litter-bags on poor fen during the decomposition experiment, while in pine bog forest one morphotype of ericoid and nine morphotypes of ectomycorrhizal roots were recorded. Molecular identification of mycorrhizal roots succeeded only in the case of one ericoid and six putatively ectomycorrhizal morphotypes. Most morphotypes were recorded only once during the whole 35-month decomposition period, and only one ericoid and one ectomycorrhizal morphotypes were shared between the poor fen and pine bog forest communities.

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