scholarly journals The role of the serotonin transporter in prefrontal cortex glutamatergic signaling following short- and long-access cocaine self-administration

2020 ◽  
pp. e12896 ◽  
Author(s):  
Lucia Caffino ◽  
Francesca Mottarlini ◽  
Boyd Van Reijmersdal ◽  
Francesca Telese ◽  
Michel M.M. Verheij ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Serotonin Transporter ◽  
Self Administration ◽  
Glutamatergic Signaling ◽  
Long Access

scholarly journals Deletion of the serotonin transporter perturbs BDNF signaling in the central amygdala following long-access cocaine self-administration

2019 ◽  
Vol 205 ◽  
pp. 107610 ◽  
Author(s):  
Lucia Caffino ◽  
Francesca Mottarlini ◽  
Danielle Mendes Diniz ◽  
Michel M. Verheij ◽  
Fabio Fumagalli ◽  
...  
Keyword(s):  
Serotonin Transporter ◽  
Central Amygdala ◽  
Self Administration ◽  
Bdnf Signaling ◽  
Long Access

scholarly journals Intermittent self-administration of fentanyl induces a multifaceted addiction state associated with persistent changes in the orexin system

2020 ◽  
Author(s):  
Jennifer E. Fragale ◽  
Morgan H. James ◽  
Gary Aston-Jones
Keyword(s):  
Behavioral Economics ◽  
Receptor Antagonist ◽  
Critical Role ◽  
Opioid Addiction ◽  
Male Rats ◽  
Self Administration ◽  
Drug Seeking ◽  
Intermittent Access ◽  
Long Access

AbstractThe orexin (hypocretin) system plays a critical role in motivated drug-taking. Cocaine self-administration with the intermittent access (IntA) procedure produces a robust addiction-like state that is orexin-dependent. Here, we sought to determine the role of the orexin system in opioid addiction using IntA self-administration of fentanyl. Different groups of male rats were either given continuous access in 1h (short access; ShA), or 6h periods (long access, LgA), or IntA (5min of access separated by 25min of no-access) to fentanyl for 14 days. IntA produced a greater escalation of fentanyl intake, motivation for fentanyl on a behavioral economics task, persistent drug seeking during abstinence, and cued-induced reinstatement compared to rats given ShA or LgA. We found that addiction behaviors induced by IntA to fentanyl were reversed by the orexin-1 receptor antagonist SB-334867. IntA to fentanyl was also associated with a persistent increase in the number of orexin-expressing neurons. Together, results indicate that the IntA model is a useful tool in the study of opioid addiction, and that the orexin system is critical for the maintenance of addiction behaviors induced by IntA self-administration of fentanyl.

scholarly journals Role of prefrontal cortex projections to the nucleus accumbens core in mediating the effects of ceftriaxone on cued cocaine relapse

2020 ◽  
Author(s):  
Allison R. Bechard ◽  
Carly N. Logan ◽  
Javier Mesa ◽  
Yasmin Padovan Hernandez ◽  
Harrison Blount ◽  
...  
Keyword(s):  
Prefrontal Cortex ◽  
Nucleus Accumbens ◽  
Basolateral Amygdala ◽  
Male Rats ◽  
Self Administration ◽  
Nucleus Accumbens Core ◽  
Cocaine Seeking ◽  
Context Cues ◽  
Glutamate Efflux

AbstractCeftriaxone is an antibiotic that reliably attenuates the reinstatement of cocaine-seeking after extinction while preventing the nucleus accumbens (NA) core glutamate efflux that drives reinstatement. However, when rats undergo abstinence without extinction, ceftriaxone attenuates context-primed relapse but NA core glutamate efflux still increases. Here we sought to determine if the same would occur when relapse is prompted by both context and discrete cues (context+cues) after cocaine abstinence. Male rats self-administered intravenous cocaine for 2 hr/day for 2 weeks. Cocaine delivery was accompanied by drug-associated cues (light+tone). Rats were then placed into abstinence with daily handling but no extinction training for two weeks. Ceftriaxone (200 mg/kg IP) or vehicle was administered during the last 6 days of abstinence. During a context+cue relapse test, microdialysis procedures were conducted. Rats were perfused at the end of the test for later Fos analysis. A separate cohort of rats was infused with the retrograde tracer cholera toxin B in the NA core and underwent the same self-administration and relapse procedures. Ceftriaxone increased baseline glutamate and attenuated both context+cue-primed relapse and NA core glutamate efflux during this test. Ceftriaxone reduced Fos expression in regions sending projections to the NA core (prefrontal cortex, basolateral amygdala, ventral tegmental area) and specifically reduced Fos in prelimbic cortex and not infralimbic cortex neurons projecting to the NA core. Thus, when relapse is primed by drug-associated cues and context, ceftriaxone is able to attenuate relapse by preventing NA core glutamate efflux, likely through reducing activity in prelimbic NA core-projecting neurons.

scholarly journals Increased cocaine self‐administration in rats lacking the serotonin transporter: a role for glutamatergic signaling in the habenula

2018 ◽  
Vol 24 (6) ◽  
pp. 1167-1178 ◽  
Author(s):  
Lucia Caffino ◽  
Michel M.M. Verheij ◽  
Lin Que ◽  
Chao Guo ◽  
Judith R. Homberg ◽  
...  
Keyword(s):  
Serotonin Transporter ◽  
Self Administration ◽  
Glutamatergic Signaling

Collection of Ego-Centered Network Data with Computer-Assisted Interviews

Methodology ◽  
2006 ◽  
Vol 2 (1) ◽  
pp. 7-15 ◽  
Author(s):  
Joachim Gerich ◽  
Roland Lehner
Keyword(s):  
Survey Methods ◽  
A Priori ◽  
Dynamic Structure ◽  
Computer Assisted ◽  
Network Data ◽  
Great Effort ◽  
Self Administration ◽  
Face To Face ◽  
Full Network

Although ego-centered network data provide information that is limited in various ways as compared with full network data, an ego-centered design can be used without the need for a priori and researcher-defined network borders. Moreover, ego-centered network data can be obtained with traditional survey methods. However, due to the dynamic structure of the questionnaires involved, a great effort is required on the part of either respondents (with self-administration) or interviewers (with face-to-face interviews). As an alternative, we will show the advantages of using CASI (computer-assisted self-administered interview) methods for the collection of ego-centered network data as applied in a study on the role of social networks in substance use among college students.

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