Survival to metamorphosis is positively related to genetic variability in a critically endangered amphibian species

2013 ◽  
Vol 17 (3) ◽  
pp. 265-274 ◽  
Author(s):  
S. C. Richter ◽  
S. O. Nunziata
2020 ◽  
Vol 10 (23) ◽  
pp. 12870-12882
Author(s):  
Igor Salles‐Oliveira ◽  
Taís Machado ◽  
Karina Rodrigues da Silva Banci ◽  
Selma M. Almeida‐Santos ◽  
Maria José de J. Silva

Primates ◽  
2016 ◽  
Vol 57 (4) ◽  
pp. 449-453 ◽  
Author(s):  
Andie Ang ◽  
Amrita Srivathsan ◽  
Rudolf Meier ◽  
Tuong Bach Luu ◽  
Quyet Khac Le ◽  
...  

2020 ◽  
Author(s):  
Thomas J. Burns ◽  
Nick Clemann ◽  
Anthony R. van Rooyen ◽  
Ben C. Scheele ◽  
Andrew R. Weeks ◽  
...  

AbstractEnvironmental DNA techniques have become established as a useful tool for biological monitoring and are used extensively to determine species presence in aquatic systems. However, their application in terrestrial systems has been more limited, likely in part due to difficulties in choosing where to sample and ensuring that collected DNA reflects current species presence. We developed methods to sample eDNA in the terrestrial environment and trialled them under controlled and field conditions. We targeted three species, an elusive critically endangered frog, an abundant non-threatened frog, and the globally distributed amphibian skin pathogen chytrid fungus, which has been implicated in the decline of over 500 amphibian species. We used a sandpaper-sampling surface to ‘trap’ DNA. After sampling, we washed the surface and filtered the wash water to gather material for DNA extraction and subsequent qPCR. Our controlled condition experiments demonstrated that frog and chytrid fungus DNA was detectable after as few as five contacts between a frog and the sampling surface. Furthermore, this DNA remained detectable after two weeks in cool, shaded, outdoor conditions. Our field experiments demonstrated that these techniques were transferable to natural habitats, where we detected both the common and rare amphibian target species, as well as chytrid fungus. Field sampling eDNA results were broadly consistent with those derived from conventional survey methods. Our methods have potential application in non-invasive sampling of amphibians and other species in terrestrial systems, broadening the applicability of eDNA techniques for species detection and monitoring.


2010 ◽  
Vol 100 (1) ◽  
pp. 05-12 ◽  
Author(s):  
Andrés Canavero ◽  
Santiago Carreira ◽  
José A. Langone ◽  
Federico Achaval ◽  
Claudio Borteiro ◽  
...  

The native species of amphibians and reptiles of Uruguay were categorized according to the IUCN Red List criteria. Out of 47 amphibian species, seven are listed as Critically Endangered (CR), five as Endangered (EN), one as Vulnerable (VU), three as Near Threatened (NT), and two as Data Deficient (DD); the remaining species are considered to be Least Concern (LC). Among the 64 species of reptiles evaluated, one is listed as Critically Endangered (CR), seven as Endangered (EN), two as Vulnerable (VU), one as Near Threatened (NT) and seven as Data Deficient (DD); the rest are considered to be Least Concern (LC). The use of these results as an additional criterion in the definition of protected areas in Uruguay will contribute towards the conservation of the aforementioned threatened species and their associated ecosystems.


2001 ◽  
Vol 15 (3) ◽  
pp. 770-775 ◽  
Author(s):  
Noviar Andayani ◽  
Juan Carlos Morales ◽  
Michael R. J. Forstner ◽  
Jatna Supriatna ◽  
Don J. Melnick

2001 ◽  
Vol 21 (6) ◽  
pp. 580-592 ◽  
Author(s):  
Arnold Boonstra ◽  
Dick de Zeeuw ◽  
Paul E. de Jong ◽  
Gerjan Navis

2013 ◽  
Vol 44 (02) ◽  
Author(s):  
H Trippe ◽  
S Lutz ◽  
A Della Marina ◽  
U Hehr ◽  
W Kress ◽  
...  
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