scholarly journals Alcohol Exposure in Utero Increases Susceptibility to Prostate Tumorigenesis in Rat Offspring

2013 ◽  
Vol 37 (11) ◽  
pp. 1901-1909 ◽  
Author(s):  
Sengottuvelan Murugan ◽  
Changqing Zhang ◽  
Sepideh Mojtahedzadeh ◽  
Dipak K. Sarkar
2017 ◽  
Vol 36 (12) ◽  
pp. 1236-1247 ◽  
Author(s):  
H Shen ◽  
K Liao ◽  
H-F Wu ◽  
H-C Lu ◽  
Y Li ◽  
...  

Objective: To investigate the effects of in utero exposure to high-dose di- n-butyl phthalate (DBP) on testicular cell apoptosis in late embryonic and pubertal male rat offspring. Methods: Twenty pregnant Sprague-Dawley (SD) rats were divided into two groups. During gestation day (GD) 12 to GD 19, control group was given 1 ml day−1 of olive oil and experimental group was given DBP 500 mg kg−1 day−1 by gavage. On GD 19.5 and postnatal day (PND) 45, the testes were removed. Morphological analysis of the testes was observed by transmission electron microscopy and hematoxylin and eosin (H&E) staining. Testicular cell apoptosis was detected by terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). The expression of Bcl-2, Bax, and p53 was presented by immunohistochemistry (IHC) and western blot. Data of the two groups was compared using independent samples t-test and Mann–Whitney test by SPSS 20.0. Results: H&E staining showed that spermatogenetic cells were significantly decreased in DBP exposed pubertal rat testis. The apoptosis index of testes in DBP-treated group was significantly lower on GD 19.5 but higher on PND 45 than that of the controls ( p < 0.01). IHC and western blot revealed significantly increased expression of Bcl-2 in GD 19.5 rat testis and Bax and p53 in PND 45 rat testis after DBP exposure, compared with the control ( p < 0.05). Conclusion: In utero exposure of high-dose DBP resulted in opposite effects on testicular cell apoptosis in late embryonic and pubertal rat offspring. The overexpression of Bcl-2, Bax, and p53 might be related to the occurrence of abnormal apoptosis and finally produce male infertility.


2005 ◽  
Vol 193 (4) ◽  
pp. 1534-1539 ◽  
Author(s):  
Laura Toso ◽  
Sarah H. Poggi ◽  
Daniel Abebe ◽  
Robin Roberson ◽  
Veronica Dunlap ◽  
...  

2019 ◽  
Vol 34 (6) ◽  
pp. 829-829
Author(s):  
D McEachern ◽  
M Steed

Abstract Objective The detrimental impact of in utero substance use on developing fetuses manifests well into adolescence (Wu, Ju, & Lu, 2011; Minnes, Lang, & Singer, 2011). Likewise, the effects of early childhood neglect during critical developmental periods may be long lasting (de Bellis, et al., 2009; Hildyard & Wolfe, 2002). Factors such as these may require creative adaptation of assessment. This case illustrates the process of evaluating a child presenting with an unusual developmental history and extremely challenging behaviors and describes efforts to maintain engagement while eliciting valid data. Successful and unsuccessful interventions will be reviewed. Method Patient is a 12-year-old Caucasian male admitted to a state psychiatric hospital with a complex presentation of emotional, behavioral, and cognitive difficulties. Aggravating factors consist of likely in utero drug and alcohol exposure, early trauma, chronic neglect, and unusually small physical stature. Neuropsychological evaluation was completed over the course of five days. Testing sessions were brief, on a unit where no one was present, and were structured using both intermittent and scheduled reinforcement to maintain effort and engagement. Results Testing indicated significant and consistent weaknesses in executive functioning, learning, memory, and attention. Despite the use of stimulant medication, difficulty with vigilance, sustained attention, and impulsivity persisted. Diagnoses were Neurodevelopmental Disorder associated with prenatal substance exposure, Generalized Anxiety Disorder, Attention Deficit/Hyperactivity Disorder, Combined presentation, and Oppositional Defiant Disorder, Moderate. Conclusions Unconventional history warrants unconventional approach to assessment. Recommendations included not only approaches to improving his attention, but just as important, strategies to elicit his best performance in various settings.


2020 ◽  
Vol 401 ◽  
pp. 115077
Author(s):  
Nathália Orlandini Costa ◽  
Simone Forcato ◽  
Andreza Manzato Cavichioli ◽  
Marina Rangel Ferro Pereira ◽  
Daniela Cristina Ceccatto Gerardin

2011 ◽  
Vol 2 (4) ◽  
pp. 239-248 ◽  
Author(s):  
Tiffany A. Polanco ◽  
Catina Crismale-Gann ◽  
Wendie S. Cohick

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