Chronic Alcohol Consumption Increases the Expression of Uncoupling Protein-2 and -4 in the Brain

2013 ◽  
pp. n/a-n/a ◽  
Author(s):  
Jan A. Graw ◽  
Clarissa von Haefen ◽  
Deniz Poyraz ◽  
Nadine Möbius ◽  
Marco Sifringer ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Uncoupling Protein ◽  
Uncoupling Protein 2 ◽  
Chronic Alcohol ◽  
Chronic Alcohol Consumption ◽  
The Brain

scholarly journals Chronic Alcohol Consumption Leads to a Tissue Specific Expression of Uncoupling Protein-2

2015 ◽  
Vol 12 (12) ◽  
pp. 995-999 ◽  
Author(s):  
Jan A. Graw ◽  
Clarissa von Haefen ◽  
Deniz Poyraz ◽  
Nadine Möbius ◽  
Marco Sifringer ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Uncoupling Protein ◽  
Uncoupling Protein 2 ◽  
Chronic Alcohol ◽  
Specific Expression ◽  
Chronic Alcohol Consumption ◽  
Tissue Specific ◽  
Tissue Specific Expression

scholarly journals Chronic alcohol-induced neuroinflammation involves CCR2/5-dependent peripheral macrophage infiltration and microglia alterations

2020 ◽  
Vol 17 (1) ◽  
Author(s):  
Patrick P. Lowe ◽  
Caroline Morel ◽  
Aditya Ambade ◽  
Arvin Iracheta-Vellve ◽  
Erica Kwiatkowski ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Immune Cell ◽  
Neuronal Damage ◽  
Microglia Activation ◽  
Macrophage Infiltration ◽  
Choice Test ◽  
Chronic Alcohol ◽  
Behavioral Alterations ◽  
Chronic Alcohol Consumption ◽  
The Brain

Abstract Background Chronic alcohol consumption is associated with neuroinflammation, neuronal damage, and behavioral alterations including addiction. Alcohol-induced neuroinflammation is characterized by increased expression of proinflammatory cytokines (including TNFα, IL-1β, and CCL2) and microglial activation. We hypothesized chronic alcohol consumption results in peripheral immune cell infiltration to the CNS. Since chemotaxis through the CCL2-CCR2 signaling axis is critical for macrophage recruitment peripherally and centrally, we further hypothesized that blockade of CCL2 signaling using the dual CCR2/5 inhibitor cenicriviroc (CVC) would prevent alcohol-induced CNS infiltration of peripheral macrophages and alter the neuroinflammatory state in the brain after chronic alcohol consumption. Methods C57BL/6J female mice were fed an isocaloric or 5% (v/v) ethanol Lieber DeCarli diet for 6 weeks. Some mice received daily injections of CVC. Microglia and infiltrating macrophages were characterized and quantified by flow cytometry and visualized using CX3CR1eGFP/+ CCR2RFP/+ reporter mice. The effect of ethanol and CVC treatment on the expression of inflammatory genes was evaluated in various regions of the brain, using a Nanostring nCounter inflammation panel. Microglia activation was analyzed by immunofluorescence. CVC-treated and untreated mice were presented with the two-bottle choice test. Results Chronic alcohol consumption induced microglia activation and peripheral macrophage infiltration in the CNS, particularly in the hippocampus. Treatment with CVC abrogated ethanol-induced recruitment of peripheral macrophages and partially reversed microglia activation. Furthermore, the expression of proinflammatory markers was upregulated by chronic alcohol consumption in various regions of the brain, including the cortex, hippocampus, and cerebellum. Inhibition of CCR2/5 decreased alcohol-mediated expression of inflammatory markers. Finally, microglia function was impaired by chronic alcohol consumption and restored by CVC treatment. CVC treatment did not change the ethanol consumption or preference of mice in the two-bottle choice test. Conclusions Together, our data establish that chronic alcohol consumption promotes the recruitment of peripheral macrophages into the CNS and microglia alterations through the CCR2/5 axis. Therefore, further exploration of the CCR2/5 axis as a modulator of neuroinflammation may offer a potential therapeutic approach for the treatment of alcohol-associated neuroinflammation.

Chronic Alcohol Exposure Alters Gene Expression and Neurodegeneration Pathways in the Brain of Adult Mice

2022 ◽  
pp. 1-17
Author(s):  
Mingjing Liu ◽  
Shipeng Guo ◽  
Daochao Huang ◽  
Dongjie Hu ◽  
Yili Wu ◽  
...  
Keyword(s):  
Energy Metabolism ◽  
Alcohol Consumption ◽  
Alcohol Exposure ◽  
Chronic Alcohol ◽  
Rna Seq ◽  
Chronic Alcohol Consumption ◽  
Mitochondrial Energy Metabolism ◽  
Brain Transcriptome ◽  
Chronic Alcohol Exposure ◽  
The Brain

Background: Chronic alcohol consumption can alter the structure of the central nervous system and disrupt cognitive function. Alcoholics are more likely to develop neurodegenerative disorders such as Alzheimer’s disease (AD) and Parkinson’s disease (PD). However, the role of alcohol in promoting neurotoxicity and neurodegeneration remains unclear. Objective: In this study, we aimed at estimating the effects of chronic binge alcohol exposure on brain transcriptome and behavior changes in a chronic “Drinking in the Dark” (DID) mouse model. Methods: The adult C57BL/6J male mice were exposed to alcohol for 4 weeks. RNA-seq was applied to assess the effects of chronic alcohol exposure on transcriptome in brain. The open field test and novel object recognition test were used to assess the changes of anxiety level, locomotive function, and short-term memory induced by alcohol. RNA-seq analysis revealed that chronic alcohol exposure caused significant change in the brain transcriptome, especially in prefrontal cortex. Results: The gene dysregulation caused by chronic alcohol exposure includes pathways related to mitochondrial energy metabolism (such as oxidative phosphorylation) and multiple neurodegenerative diseases (such as AD and PD). Furthermore, the pathway and network analyses suggest that the genes involved in mitochondrial energy metabolism, ubiquitin-proteasome system, Wnt signaling pathway, and microtubules may attribute to the neurotoxicity and neurodegeneration caused by chronic alcohol consumption. Additionally, locomotive function was also significantly impaired. Conclusion: This work provides gene transcriptional profile data for future research on alcohol-induced neurodegenerative diseases, especially AD and PD.

Effects of chronic alcohol consumption on the expression of different NR1 splice variants in the brain of AA and ANA lines of rats

1999 ◽  
Vol 72 (2) ◽  
pp. 166-175 ◽  
Author(s):  
Anett Winkler ◽  
Beatrice Mahal ◽  
Kalervo Kiianmaa ◽  
Walter Zieglgänsberger ◽  
Rainer Spanagel
Keyword(s):  
Alcohol Consumption ◽  
Splice Variants ◽  
Chronic Alcohol ◽  
Chronic Alcohol Consumption ◽  
The Brain

Decreased Hepatotoxicity of Dimethylnitrosamine (DMN) Following Chronic Alcohol Consumption

1980 ◽  
pp. 237-243
Author(s):  
J. Gellert ◽  
F. Moreno ◽  
M. Haydn ◽  
H. Oldiges ◽  
H. Frenzel ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Chronic Alcohol ◽  
Chronic Alcohol Consumption

scholarly journals Chronic Alcohol Consumption Blunts β-Adrenergic Responsiveness in Left Ventricular Cardiomyocytes

2010 ◽  
Vol 98 (3) ◽  
pp. 518a
Author(s):  
Krista N. Blackwell ◽  
Dennis J. Rozanski ◽  
Dominique C. Renard-Rooney ◽  
Andrew P. Thomas
Keyword(s):  
Alcohol Consumption ◽  
Left Ventricular ◽  
Chronic Alcohol ◽  
Chronic Alcohol Consumption ◽  
Ventricular Cardiomyocytes

scholarly journals M2b Macrophage Elimination and Improved Resistance of Mice with Chronic Alcohol Consumption to Opportunistic Infections

2015 ◽  
Vol 185 (2) ◽  
pp. 420-431 ◽  
Author(s):  
Hideko Ohama ◽  
Akira Asai ◽  
Ichiaki Ito ◽  
Sumihiro Suzuki ◽  
Makiko Kobayashi ◽  
...  
Keyword(s):  
Alcohol Consumption ◽  
Opportunistic Infections ◽  
Chronic Alcohol ◽  
Chronic Alcohol Consumption
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