scholarly journals NAP1L2 drives mesenchymal stem cell senescence and suppresses osteogenic differentiation

Aging Cell ◽  
2022 ◽  
Author(s):  
Meilin Hu ◽  
Liangyu Xing ◽  
Li Zhang ◽  
Fan Liu ◽  
Sheng Wang ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Senescence

scholarly journals P-cresol and Indoxyl Sulfate Impair Osteogenic Differentiation by Triggering Mesenchymal Stem Cell Senescence

2021 ◽  
Vol 18 (3) ◽  
pp. 744-755
Author(s):  
Witchayapon Kamprom ◽  
Tulyapruek Tawonsawatruk ◽  
Sumana Mas-Oodi ◽  
Korrarit Anansilp ◽  
Manoch Rattanasompattikul ◽  
...  
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Senescence ◽  
Indoxyl Sulfate

scholarly journals Melatonin promotes bone marrow mesenchymal stem cell osteogenic differentiation and prevents osteoporosis development through modulating circ_0003865 that sponges miR-3653-3p

2021 ◽  
Vol 12 (1) ◽  
Author(s):  
Xudong Wang ◽  
Taiqiu Chen ◽  
Zhihuai Deng ◽  
Wenjie Gao ◽  
Tongzhou Liang ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Luciferase Reporter ◽  
Specific Gene ◽  
Biological Processes ◽  
Alizarin Red ◽  
Qrt Pcr ◽  
Dual Luciferase Reporter Assay

Abstract Background Little is known about the implications of circRNAs in the effects of melatonin (MEL) on bone marrow mesenchymal stem cell (BMSC) osteogenic differentiation and osteoporosis (OP) progression. The aim of our study was to investigate circRNAs in MEL-regulated BMSC differentiation and OP progression. Methods BMSC osteogenic differentiation was measured by qRT-PCR, western blot (WB), Alizarin Red, and alkaline phosphatase (ALP) staining. Differential circRNA and mRNA profiles of BMSCs treated by MEL were characterized by deep sequencing, followed by validation using RT-PCR, Sanger sequencing, and qRT-PCR. Silencing and overexpression of circ_0003865 were conducted for functional investigations. The sponged microRNAs and targeted mRNAs were predicted by bioinformatics and validated by qRT-PCR, RNA pull-down, and dual-luciferase reporter assay. The function of miR-3653-3p and circ_0003865/miR-3653-3p/growth arrest-specific gene 1 (GAS1) cascade was validated for the osteogenic differentiation of BMSCs by CCK-8, qRT-PCR, WB, Alizarin Red, and ALP staining. The effects of circ_0003865 on OP development were tested in murine OP model. Results MEL promoted osteogenic differentiation of BMSCs. RNA sequencing revealed significant alterations in circRNA and mRNA profiles associated with multiple biological processes and signaling pathways. Circ_0003865 expression in BMSCs was significantly decreased by MEL treatment. Silencing of circ_0003865 had no effect on proliferation while promoted osteogenic differentiation of BMSCs. Overexpression of circ_0003865 abrogated the promotion of BMSC osteogenic differentiation induced by MEL, but proliferation of BMSCs induced by MEL had no change whether circ_0003865 was overexpression or not. Furthermore, circ_0003865 sponged miR-3653-3p to promote GAS1 expression in BMSCs. BMSC osteogenic differentiation was enhanced by miR-3653-3p overexpression while BMSC proliferation was not affected. By contrast, miR-3653-3p silencing mitigated the promoted BMSC osteogenic differentiation caused by circ_0003865 silencing, but had no effect on proliferation. Finally, circ_0003865 silencing repressed OP development in mouse model. Conclusion MEL promotes BMSC osteogenic differentiation and inhibits OP pathogenesis by suppressing the expression of circ_0003865, which regulates GAS1 gene expression via sponging miR-3653-3p.

scholarly journals Mesenchymal stem cell-derived exosomes have altered microRNA profiles and induce osteogenic differentiation depending on the stage of differentiation

PLoS ONE ◽  
2018 ◽  
Vol 13 (2) ◽  
pp. e0193059 ◽  
Author(s):  
Xiaoqin Wang ◽  
Omar Omar ◽  
Forugh Vazirisani ◽  
Peter Thomsen ◽  
Karin Ekström
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation

Functions of the Mg–HA coating on carbon/carbon composite surface to promote the proliferation and osteogenic differentiation of mBMSCs

RSC Advances ◽  
2016 ◽  
Vol 6 (107) ◽  
pp. 105056-105062 ◽  
Author(s):  
Xiong Xinbo ◽  
Ni Xinye ◽  
Zhou Dong
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Carbon Composite ◽  
Composite Surface ◽  
Ha Coating

This study aims to evaluate the functions of the Mg–hydroxyapatite (Mg–HA) bio-coating on carbon/carbon composite (C/C) surface to promote the proliferation and osteogenic differentiation of bone mesenchymal stem cell (BMSCs).

Regulation of the mesenchymal stem cell fate by interleukin-17: Implications in osteogenic differentiation

2021 ◽  
Vol 13 (11) ◽  
pp. 1699-1716
Author(s):  
Jelena Krstić ◽  
Slavko Mojsilović ◽  
Sonja S Mojsilović ◽  
Juan F Santibanez
Keyword(s):  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Osteogenic Differentiation ◽  
Cell Fate ◽  
Interleukin 17 ◽  
Stem Cell Fate

scholarly journals Nicotinamide phosphoribosyltransferase postpones rat bone marrow mesenchymal stem cell senescence by mediating NAD+–Sirt1 signaling

Aging ◽  
2019 ◽  
Vol 11 (11) ◽  
pp. 3505-3522 ◽  
Author(s):  
Chenchen Pi ◽  
Yue Yang ◽  
Yanan Sun ◽  
Huan Wang ◽  
Hui Sun ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Stem Cell ◽  
Mesenchymal Stem Cell ◽  
Cell Senescence ◽  
Nicotinamide Phosphoribosyltransferase ◽  
Rat Bone
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