scholarly journals Chemically induced senescence in human stem cell‐derived neurons promotes phenotypic presentation of neurodegeneration

Aging Cell ◽  
2021 ◽  
Author(s):  
Ali Fathi ◽  
Sakthikumar Mathivanan ◽  
Linghai Kong ◽  
Andrew J. Petersen ◽  
Cole R. K. Harder ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Stem Cell ◽  
Chemically Induced

scholarly journals Chemically Induced Senescence in Human Stem Cell-Derived Neurons Promotes Phenotypic Presentation of Neurodegeneration

2021 ◽  
Author(s):  
Ali Fathi ◽  
Sakthikumar Mathivanan ◽  
Linghai Kong ◽  
Andrew J Petersen ◽  
Cole R.K Harder ◽  
...  
Keyword(s):  
Stem Cell ◽  
Small Molecules ◽  
Motor Neurons ◽  
Cortical Neurons ◽  
Axonal Degeneration ◽  
Human Stem Cell ◽  
Human Stem Cells ◽  
Embryonic Fibroblasts ◽  
Age Related ◽  
Chemically Induced

Modeling age-related neurodegenerative disorders with human stem cells is difficult due to the embryonic nature of stem cell derived neurons. We developed a chemical cocktail to induce senescence of iPSC-derived neurons to address this challenge. We first screened small molecules that induce embryonic fibroblasts to exhibit features characteristic of aged fibroblasts. We then optimized a cocktail of small molecules that induced senescence in fibroblasts and cortical neurons without causing DNA damage. The utility of the senescence cocktail was validated in motor neurons derived from ALS patient iPSCs which exhibited protein aggregation and axonal degeneration substantially earlier than those without cocktail treatment. Our senescence cocktail will likely enhance the manifestation of disease-related phenotypes in neurons derived from iPSCs, enabling the generation of reliable drug discovery platforms.

Chemically induced hepatotoxicity in human stem cell-induced hepatocytes compared with primary hepatocytes and HepG2

2016 ◽  
Vol 32 (5) ◽  
pp. 403-417 ◽  
Author(s):  
Seok-Jin Kang ◽  
Hyuk-Mi Lee ◽  
Young-Il Park ◽  
Hee Yi ◽  
Hunjoo Lee ◽  
...  
Keyword(s):  
Stem Cell ◽  
Human Stem Cell ◽  
Primary Hepatocytes ◽  
Chemically Induced

An Update on Human Stem Cell-Based Therapy in Parkinson';s Disease

2016 ◽  
Vol 11 (7) ◽  
pp. 561-568 ◽  
Author(s):  
Clara González ◽  
Sonia Bonilla ◽  
Ana Isabel Flores ◽  
Eva Cano ◽  
Isabel Liste
Keyword(s):  
Stem Cell ◽  
Human Stem Cell ◽  
Cell Based Therapy

scholarly journals A hydrogel platform for in vitro three dimensional assembly of human stem cell-derived islet cells and endothelial cells

2019 ◽  
Vol 97 ◽  
pp. 272-280 ◽  
Author(s):  
Punn Augsornworawat ◽  
Leonardo Velazco-Cruz ◽  
Jiwon Song ◽  
Jeffrey R. Millman
Keyword(s):  
Endothelial Cells ◽  
Stem Cell ◽  
Islet Cells ◽  
Three Dimensional ◽  
Human Stem Cell
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