scholarly journals Age‐related changes in metabolites in young donor livers and old recipient sera after liver transplantation from young to old rats

Aging Cell ◽  
2021 ◽  
Author(s):  
Qunhua Han ◽  
Hui Li ◽  
Mengyuan Jia ◽  
Lin Wang ◽  
Yulan Zhao ◽  
...  
Keyword(s):  
Liver Transplantation ◽  
Young Donor ◽  
Age Related ◽  
Old Rats ◽  
Age Related Changes

Age-related changes in endothelial permeability and distribution volume of albumin in rat aorta

1993 ◽  
Vol 264 (3) ◽  
pp. H679-H685 ◽  
Author(s):  
J. Belmin ◽  
B. Corman ◽  
R. Merval ◽  
A. Tedgui
Keyword(s):  
Arterial Wall ◽  
Relative Concentration ◽  
Endothelial Permeability ◽  
Distribution Volume ◽  
Albumin Concentration ◽  
Macromolecular Transport ◽  
Age Related ◽  
The Media ◽  
Old Rats ◽  
Age Related Changes

Age-related changes in macromolecular transport across the arterial wall were investigated in 10-, 20-, and 30-mo-old WAG/Rij rats. Animals were injected with 125I- and 131I-labeled albumin, 90 and 5 min before they were killed, respectively. The transmural distribution of relative concentration of tracers in the aortic wall was obtained using en face serial sectioning technique. The apparent endothelial permeability to albumin calculated from the distribution of 5-min 131I-labeled albumin concentrations was significantly enhanced in 20- and 30-mo-old rats compared with 10-mo-old rats. The apparent distribution volume of albumin within the media, estimated as the mean medial 125I-labeled albumin concentration, was not significantly changed in 20-mo-old rats but was significantly decreased in the 30-mo-old animals. These age-related changes in the macromolecular transport suggest that the entry of plasma macromolecules in the aged arterial wall might be enhanced, whereas the efflux through the media may be impeded, possibly contributing to their trapping in the subendothelium.

Age-related changes in substance P-immunoreactive nerve structures of the rat recto-anal region

Open Medicine ◽  
2010 ◽  
Vol 5 (3) ◽  
pp. 358-364
Author(s):  
Nikolai Lazarov ◽  
Dimitar Itzev ◽  
Kamen Usunoff ◽  
Negrin Negrev ◽  
Radomir Radomirov
Keyword(s):  
Substance P ◽  
Anal Sphincter ◽  
Internal Anal Sphincter ◽  
Circular Muscle ◽  
Muscle Layer ◽  
Nerve Fibres ◽  
Anal Region ◽  
Age Related ◽  
Old Rats ◽  
Age Related Changes

AbstractThe recto-anal region is innervated by extrinsic and intrinsic nerves and a number of neuropeptides including substance P (SP) have been suggested to participate in the regulation of intestinal movements. We examined the age-related changes in the distribution of SP-immunoreactive nerve structures in the distal part of the rat large intestine. Using immunohistochemistry, the presence of SP was studied in fresh tissues from Wistar rats at different ages taken at three sampling sites, the distal rectum, anal canal and internal anal sphincter. In the 15-day old rats the myenteric plexus of the distal rectum and anal canal was well outlined by numerous SP-immunoreactive varicose nerve fibres encircling immunonegative perikarya. In the circular muscle layer, nerve fibres and small nerve bundles ran parallel to the muscle cells, while in the longitudinal muscle layer, only occasional nerve fibres were seen. At the level of the internal anal sphincter, no myenteric ganglia were present. Here, thin varicose fibers ran parallel to the smooth muscle cells. In the 3-month old rats, a larger number of intensely staining SP-immunoreactive nerve fibres were found and in the circular muscle layer, thicker nerve strands were observed. In the 26-month old rats, the density and staining intensity of SP-immunopositive nerve fibres in the myenteric plexus was lower than in the 3-month-old rats. Similar changes in the SP-immunostained fibres in the internal anal sphincter were observed. Degenerative alterations in SP-containing fibres during aging appear to play a role in ano-rectal motility and sphincter control.

Circulating hyaluronan levels in the rodent: effects of age and diet

1995 ◽  
Vol 268 (4) ◽  
pp. C952-C957 ◽  
Author(s):  
J. Yannariello-Brown ◽  
S. H. Chapman ◽  
W. F. Ward ◽  
T. C. Pappas ◽  
P. H. Weigel
Keyword(s):  
Fish Meal ◽  
Caloric Intake ◽  
Protein Source ◽  
Male Rats ◽  
Semisynthetic Diet ◽  
Fischer 344 ◽  
Age Related ◽  
Old Rats ◽  
Ad Libitum ◽  
Age Related Changes

Circulating hyaluronan (HA) levels were investigated as a function of age and diet in Fischer 344 male rats. A biphasic pattern of age-related changes was observed in rats fed ad libitum a diet in which the protein source was soya/fish meal. HA levels in 3- to 6- and 22- to 29-mo-old rats were not statistically different. However, HA levels in 12- to 20-mo-old rats were 10-29% of the levels in younger or aged adults. HA levels were also measured in rats fed ad libitum a semisynthetic diet in which the protein source was hydrolyzed casein. Whereas the two colonies exhibited similar biphasic age-related changes, HA levels differed 4- to 20-fold at every age examined. Caloric restriction affected HA levels in 19-mo-old casein-fed rats; HA levels were 2.3 times higher than age-matched controls and were not statistically different from young or aged animals. Serum and plasma HA levels were identical in the same individuals at all ages tested. These data suggest that HA turnover and metabolism in the rat are affected by age, dietary composition, and caloric intake.

scholarly journals Age-Related Changes in Sirtuin 7 Expression in Calorie-Restricted and Refed Rats

Gerontology ◽  
2015 ◽  
Vol 62 (3) ◽  
pp. 304-310 ◽  
Author(s):  
Agata Wronska ◽  
Aleksandra Lawniczak ◽  
Piotr M. Wierzbicki ◽  
Zbigniew Kmiec
Keyword(s):  
Gene Expression ◽  
Protein Expression ◽  
Calorie Restriction ◽  
Ribosome Biogenesis ◽  
Healthy Aging ◽  
Mrna Levels ◽  
Age Related ◽  
Mrna And Protein Expression ◽  
Old Rats ◽  
Age Related Changes

Background: Sirtuins (SIRT1-7) have been implicated to mediate the beneficial effects of calorie restriction for healthy aging. While the physiological functions of SIRT7 are still poorly understood, SIRT7 has recently been shown to affect ribosome biogenesis, mitochondrial gene expression, and hepatic lipid metabolism. Objective: To analyze the effects of age and short-term calorie restriction (SCR) and subsequent refeeding on SIRT7 expression in key metabolic tissues. Methods: Four- and 24-month-old male Wistar rats were subjected to 40% SCR for 30 days, followed by ad libitum feeding for 2 or 4 days. Liver, white adipose tissue (WAT), heart and skeletal muscle samples were analyzed by real-time PCR and Western blotting for SIRT7 mRNA and protein expression, respectively. Results: Aging had diverse effects on SIRT7 levels in lipogenic tissues: both the mRNA and protein levels increased in the retroperitoneal depot (rWAT), did not change in the epididymal depot (eWAT), and decreased in the subcutaneous depot (sWAT) and the liver of old as compared to young animals. In the heart, extensor digitorum longus muscle (EDL) and soleus muscle (SOL), Sirt7 gene but not protein expression was lower in old than in young control rats. SCR did not affect SIRT7 expression in WAT and the liver in both age groups. In the heart of young animals, SCR did not affect SIRT7 mRNA or protein level. In EDL, SIRT7 protein but not mRNA levels decreased after SCR and remained reduced upon refeeding. In SOL, both SIRT7 mRNA and protein expression were inhibited by refeeding. In old rats, cardiac Sirt7 expression increased after SCR and refeeding. In old rats' EDL and SOL muscles, SIRT7 protein expression was inhibited by refeeding. Conclusion: Age-related changes of SIRT7 gene expression in key organs of energy homeostasis are tissue dependent.

360 AGE-RELATED CHANGES IN SPLENIC NEUROTROPHIN CONTENT: A POSSIBLE CAUSE FOR DECLINING SYMPATHETIC INNERVATION IN OLD RATS

2005 ◽  
Vol 53 (1) ◽  
pp. S141.3-S141
Author(s):  
K. Bassett ◽  
C. A. Molinaro ◽  
A. B. Millar ◽  
S. Flowers ◽  
S. D. Perez
Keyword(s):  
Sympathetic Innervation ◽  
Age Related ◽  
Old Rats ◽  
Possible Cause ◽  
Age Related Changes

scholarly journals Vocal Training Mitigates Age-Related Changes Within the Vocal Mechanism in Old Rats

2013 ◽  
Vol 68 (12) ◽  
pp. 1458-1468 ◽  
Author(s):  
Aaron M. Johnson ◽  
Michelle R. Ciucci ◽  
Nadine P. Connor
Keyword(s):  
Vocal Training ◽  
Age Related ◽  
Old Rats ◽  
Age Related Changes

Age-related changes in body composition are associated with hepatic insulin resistance in conscious rats

1996 ◽  
Vol 270 (6) ◽  
pp. E930-E936 ◽  
Author(s):  
N. Barzilai ◽  
L. Rossetti
Keyword(s):  
Insulin Resistance ◽  
Body Composition ◽  
Glucose Metabolism ◽  
Hepatic Glucose Production ◽  
Maximum Velocity ◽  
Hepatic Insulin Resistance ◽  
Age Related ◽  
Hepatic Glucose ◽  
Old Rats ◽  
Age Related Changes

Age-dependent changes in body composition and hepatic ([3H]glucose) glucose metabolism were examined in 2-, 4-, and 14-mo-old (n = 26) conscious Sprague-Dawley rats. Hepatic glucose production (HGP) and hepatic glucose-6-phosphatase maximum velocity were decreased 18 and 30%, respectively, between 2 and 4 mo but were unchanged with further aging. However, between 4 and 14 mo, twofold higher plasma insulin levels were required to maintain similar HGP, suggesting that hepatic insulin resistance develops with age. Utilizing hepatic-pancreatic clamp technique, we showed that a much higher rate of insulin infusion (1.6 +/- 0.1 vs. 0.8 +/- 0.1 mU.kg-1.min-1) was needed to achieve similar plasma glucose levels and HGP. Furthermore, when 4-mo-old rats were infused with insulin at similar rates as the 14-mo-old rats, HGP was decreased by approximately 30%. Because hepatic insulin sensitivity was inversely related to the increase in body weight (r2 = 0.876) and free fatty acid levels (r2 = 0.843), we suggest that age-related changes in body composition may lead to the impairment of hepatic glucose metabolism.

scholarly journals Age-related reductions in the number of serial sarcomeres contribute to shorter fascicle lengths but not elevated passive tension

2020 ◽  
Author(s):  
Geoffrey A. Power ◽  
Sean Crooks ◽  
Jared R. Fletcher ◽  
Brian R. Macintosh ◽  
Walter Herzog
Keyword(s):  
Muscle Length ◽  
Medial Gastrocnemius ◽  
Slight Reduction ◽  
Passive Tension ◽  
Passive Force ◽  
Older Individuals ◽  
Age Related ◽  
Old Rats ◽  
Length Changes ◽  
Age Related Changes

AbstractWe investigated age-related changes to fascicle length (FL), sarcomere length (SL), and serial sarcomere number (SSN), and how this affects passive force. Following mechanical testing to determine passive force, the medial gastrocnemius muscle of young (n=9) and old (n=8) Fisher 344BN hybrid rats was chemically fixed at the optimal muscle length for force production; individual fascicles were dissected for length measurement, and laser diffraction was used to assess SL. Old rats had ∼14% shorter FL than young, which was driven by a ∼10% reduction in SSN, with no difference in SL (∼4%). Passive force was greater in the old compared to young rats at long muscle lengths. Shorter FL and reduced SSN in the old rats could not entirely explain increased passive forces for absolute length changes, owing to a slight reduction in SL in old, resulting in similar SL at long muscle lengths.Summary StatementThis study sought to explain the increased passive tension observed for muscles of older individuals owing to age-related changes to muscle architecture.

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