scholarly journals Alzheimer’s amyloid β heterogeneous species differentially affect brain endothelial cell viability, blood‐brain barrier integrity, and angiogenesis

Aging Cell ◽  
2020 ◽  
Vol 19 (11) ◽  
Author(s):  
Rebecca Parodi‐Rullán ◽  
Jorge Ghiso ◽  
Erwin Cabrera ◽  
Agueda Rostagno ◽  
Silvia Fossati
Keyword(s):  
Endothelial Cell ◽  
Cell Viability ◽  
Blood Brain Barrier ◽  
Amyloid Β ◽  
Brain Barrier ◽  
Brain Endothelial Cell ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity

scholarly journals Effect of heat stress on blood-brain barrier integrity in iPS cell-derived microvascular endothelial cell models

PLoS ONE ◽  
2019 ◽  
Vol 14 (9) ◽  
pp. e0222113 ◽  
Author(s):  
Tomoko Yamaguchi ◽  
Kentaro Shimizu ◽  
Yasuhiro Kokubu ◽  
Misae Nishijima ◽  
Shuko Takeda ◽  
...  
Keyword(s):  
Heat Stress ◽  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Microvascular Endothelial Cell ◽  
Cell Models ◽  
Ips Cell ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity

scholarly journals Monitoring Blood-Brain Barrier Integrity Following Amyloid-β Immunotherapy Using Gadolinium-Enhanced MRI in a PDAPP Mouse Model

2016 ◽  
Vol 54 (2) ◽  
pp. 723-735 ◽  
Author(s):  
Ines Blockx ◽  
Steve Einstein ◽  
Pieter-Jan Guns ◽  
Johan Van Audekerke ◽  
Caroline Guglielmetti ◽  
...  
Keyword(s):  
Mouse Model ◽  
Blood Brain Barrier ◽  
Amyloid Β ◽  
Brain Barrier ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Enhanced Mri ◽  
Blood Brain Barrier Integrity

scholarly journals Mice deficient in endothelial α5 integrin are profoundly resistant to experimental ischemic stroke

2016 ◽  
Vol 37 (1) ◽  
pp. 85-96 ◽  
Author(s):  
Jill Roberts ◽  
Leon de Hoog ◽  
Gregory J Bix
Keyword(s):  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Infarct Volume ◽  
Ischemic Injury ◽  
Glucose Deprivation ◽  
Brain Barrier ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity

Stroke is a disease in dire need of better therapies. We have previously shown that a fragment of the extracellular matrix proteoglycan, perlecan, has beneficial effects following cerebral ischemia via the α5β1 integrin receptor. We now report that endothelial cell selective α5 integrin deficient mice (α5 KO) are profoundly resistant to ischemic infarct after transient middle cerebral artery occlusion. Specifically, α5 KOs had little to no infarct 2–3 days post-stroke, whereas controls had an increase in mean infarct volume over the same time period as expected. Functional outcome is also improved in the α5 KOs compared with controls. Importantly, no differences in cerebrovascular anatomy or collateral blood flow were noted that could account for this difference in ischemic injury. Rather, we demonstrate that α5 KOs have increased blood-brain barrier integrity (increased expression of claudin-5, and absent brain parenchymal IgG extravasation) after stroke compared with controls, which could explain their resistance to ischemic injury. Additionally, inhibition of α5 integrin in vitro leads to decreased permeability of brain endothelial cells following oxygen-glucose deprivation. Together, these findings indicate endothelial cell α5 integrin plays an important role in stroke outcome and blood-brain barrier integrity, suggesting that α5 integrin could be a novel therapeutic target for stroke.

scholarly journals Multimodal investigations of trans-endothelial cell trafficking under condition of disrupted blood-brain barrier integrity

2010 ◽  
Vol 11 (1) ◽  
pp. 34 ◽  
Author(s):  
Nicola Marchi ◽  
Qingshan Teng ◽  
Minh T Nguyen ◽  
Linda Franic ◽  
Nirav K Desai ◽  
...  
Keyword(s):  
Endothelial Cell ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Cell Trafficking ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Blood Brain Barrier Integrity

scholarly journals Glucocorticoid effects on endothelial barrier function in the murine brain endothelial cell line cEND incubated with sera from patients with multiple sclerosis

2010 ◽  
Vol 16 (3) ◽  
pp. 293-302 ◽  
Author(s):  
Kinga G Blecharz ◽  
Aiden Haghikia ◽  
Mariusz Stasiolek ◽  
Niels Kruse ◽  
Detlev Drenckhahn ◽  
...  
Keyword(s):  
Gene Expression ◽  
Multiple Sclerosis ◽  
Endothelial Cell ◽  
Tight Junction ◽  
Blood Brain Barrier ◽  
Brain Barrier ◽  
Barrier Integrity ◽  
Blood Brain ◽  
Junction Protein ◽  
Blood Brain Barrier Integrity

Compromised blood—brain barrier integrity is a major hallmark of active multiple sclerosis (MS). Alterations in brain endothelial tight junction protein and gene expression occur early during neuroinflammation but there is little known about the underlying mechanisms. In this study, we analysed barrier compromising effects of sera from MS patients and barrier restoring effects of glucocorticoids on blood—brain barrier integrity in vitro. cEND murine brain microvascular endothelial cell monolayers were incubated with sera from patients in active phase of disease or in relapse. Data were compared with effects of the glucocorticoid dexamethasone alone or in combination with MS sera on barrier integrity. Tight junction protein levels and gene expression were evaluated concomitant with barrier integrity. We reveal downregulation of claudin-5 and occludin protein and mRNA and an accompanying upregulation in expression of matrix metalloproteinase MMP-9 after incubation with serum from active disease and remission and also a minor reconstitution of barrier functions related to dexamethasone treatment. Moreover, we for the first time describe downregulation of claudin-5 and occludin protein after incubation of cEND cells with sera from patients in remission phase of MS. Our findings reveal direct and differential effects of MS sera on blood-brain barrier integrity.

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