scholarly journals Follicle‐stimulating hormone promotes renal tubulointerstitial fibrosis in aging women via the AKT/GSK‐3β/β‐catenin pathway

Aging Cell ◽  
2019 ◽  
Vol 18 (5) ◽  
Author(s):  
Kun Zhang ◽  
Lin Kuang ◽  
Fangzhen Xia ◽  
Yi Chen ◽  
Wen Zhang ◽  
...  
Keyword(s):  
Follicle Stimulating Hormone ◽  
Tubulointerstitial Fibrosis ◽  
Renal Tubulointerstitial Fibrosis ◽  
Aging Women ◽  
Gsk 3Β ◽  
Stimulating Hormone

scholarly journals HuangQi Decoction Improves Renal Tubulointerstitial Fibrosis in Mice by Inhibiting the Up-Regulation of Wnt/β-Catenin Signaling Pathway

2015 ◽  
Vol 36 (2) ◽  
pp. 655-669 ◽  
Author(s):  
Ming-Qian Jiang ◽  
Li Wang ◽  
Ai-li Cao ◽  
Jie Zhao ◽  
Xia Chen ◽  
...  
Keyword(s):  
Signaling Pathway ◽  
Sham Group ◽  
Interstitial Fibrosis ◽  
Immunohistochemical Analysis ◽  
Tubulointerstitial Fibrosis ◽  
Rt Pcr ◽  
Renal Interstitial Fibrosis ◽  
Expression Levels ◽  
Renal Tubulointerstitial Fibrosis ◽  
Gsk 3Β

Object: To explore the effects of HuangQi decoction on tubulointerstitial fibrosis in mice and the Wnt/β-catenin signaling pathway. Methods: Unilateral ureteral obstruction (UUO) model was used. A total of 120 C57/BL mice were randomly divided into 6 groups, sham group, sham+HuangQi decoction group (1.08 g/kg), UUO group, UUO+HuangQi decoction group (0.12, 0.36, 1.08 g/kg). Immunohistochemical analysis, RT-PCR and Western blot were employed to examine the proteins and genes related to the Wnt/β-catenin signaling pathway. Results: In UUO mice models, expression levels of Wnt3,4, Frizzled4, LRP5,6, β-catenin, LEF-1, TCF-1, Snail, MMP2,7 genes were positively correlated with the degree of renal tubulointerstitial fibrosis, while expression levels of GSK-3β, Axin, APC, CK1 were negatively correlated. HuangQi decoction could down-regulate expression levels of Wnt3,4, Frizzled4, LRP5,6, β-catenin, LEF-1, TCF-1, Snail, Twist, MMP2,7 and up-regulate expression levels of GSK-3β, Axin, APC, CK1 and E-cadherin. Conclusion: HuangQi decoction could effectively inhibit the up-regulation of Wnt/β-catenin signaling pathway induced by UUO, implying a possible role in improving renal interstitial fibrosis.

Follicle-stimulating hormone-dependent estrogen secretion by rat Sertoli cells in vitro: Modulation by calcium

1991 ◽  
Vol 125 (3) ◽  
pp. 280-285 ◽  
Author(s):  
J. Alan Talbot ◽  
Ann Lambert ◽  
Robert Mitchell ◽  
Marek Grabinski ◽  
David C. Anderson ◽  
...  
Keyword(s):  
Sertoli Cells ◽  
Culture Medium ◽  
Follicle Stimulating Hormone ◽  
Dibutyryl Camp ◽  
Immature Rat ◽  
Estradiol Secretion ◽  
Old Rats ◽  
Stimulating Hormone

Abstract We have investigated the role of Ca2+ in the control of FSH-induced estradiol secretion by Sertoli cells isolated from 8-10 days old rats. Exogenous Ca2+ (4-8 mmol/1) inhibited FSH-stimulated E2 secretion such that, with 8 mmol/l Ca2+ and FSH (8 IU/l) E2 secretion decreased from 2091±322 to 1480±84 pmol/l (p<0.002), whilst chelation of Ca2+ in the culture medium with EGTA (3 mmol/l) increased E2 secretion from 360±45 to 1242±133 pmol/l) in the absence of FSH. Further, EGTA (3 mmol/l) markedly potentiated FSH (8 IU/l), forskolin (1 μmol/l) and dibutyryl cAMP (1 mmol/l)-stimulated E2 secretion. Addition of the Ca2+ ionophores, ionomycin (2-5 μmol/l) and A23187 (2 μmol/l), inhibited FSH (8 IU/l)-stimulated E2 secretion by >80%. The effect of ionomycin was totally reversible, whereas that of A23187 was irreversible. Ionomycin (5 μmol/l) had no effect on EGTA-induced E2 secretion in the absence of FSH, but reduced EGTA-provoked E2 secretion by 59% in the presence of FSH (8 IU/l). Similarly, forskolin- and dibutyryl cAMP-provoked E2 production was inhibited 46-50% by ionomycin (5 μmol/l). We conclude that FSH-induced E2 secretion from immature rat Sertoli cells is modulated by intra- and extracellular Ca2+.

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