scholarly journals Ca v 1.2 regulates osteogenesis of bone marrow‐derived mesenchymal stem cells via canonical Wnt pathway in age‐related osteoporosis

Aging Cell ◽  
2019 ◽  
Vol 18 (4) ◽  
Author(s):  
Dongdong Fei ◽  
Yang Zhang ◽  
Junjie Wu ◽  
Hui Zhang ◽  
Anqi Liu ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Wnt Pathway ◽  
Canonical Wnt Pathway ◽  
Age Related ◽  
Canonical Wnt

scholarly journals Efficiency of Cell Therapy to GC-Induced ONFH: BMSCs with Dkk-1 Interference Is Not Superior to Unmodified BMSCs

2018 ◽  
Vol 2018 ◽  
pp. 1-9 ◽  
Author(s):  
Wei Zhun ◽  
Li Donghai ◽  
Yang Zhouyuan ◽  
Zhao Haiyan ◽  
Kang Pengde
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Cell Therapy ◽  
Wnt Pathway ◽  
Allograft Bone ◽  
Canonical Wnt Pathway ◽  
Canonical Wnt ◽  
Dickkopf 1

Glucocorticoid-induced osteonecrosis of the femoral head (ONFH) is a hip disorder, and it threatens patients who require megadose of steroid therapies. Nowadays, no valid therapies can reverse the development of GC-induced ONFH once it occurs. Stem cell therapy to GC-induced ONFH would be a promising choice. Although the pathogenesis of GC-induced ONFH is not yet fully clear, Dickkopf-1 (Dkk-1) upregulated by excessive GC use, which hinders the canonical Wnt pathway, could be an explanation. Thus, the aim of the present work lies in investigating the efficiency of the allograft bone marrow stem cells (BMSCs) with Dkk-1 interference in preventing the progression of the GC-induced ONFH. Lentivirus-meditated Dkk-1 RNAi was introduced into BMSCs which was exposed to dexamethasone (10−6 mol/L) in vitro. This interference blocked Dkk-1 overexpression by GC and afterwards prompted the transduction of Wnt/β-catenin in which the Runx2 and PPARγ were upregulated and downregulated, respectively. Thus, the osteogenesis was promoted while adipogenesis was inhibited. In vivo, GC-induced ONFH rats were treated by allotransplantation of BMSCs with Dkk-1 interference, and the progression of the disease was prevented. However, the effects were not significantly superior to treatment with nongenetically modified or normal BMSCs.

scholarly journals Age-related intrinsic changes in human bone-marrow-derived mesenchymal stem cells and their differentiation to osteoblasts

Aging Cell ◽  
2008 ◽  
Vol 7 (3) ◽  
pp. 335-343 ◽  
Author(s):  
Shuanhu Zhou ◽  
Joel S. Greenberger ◽  
Michael W. Epperly ◽  
Julie P. Goff ◽  
Carolyn Adler ◽  
...  
Keyword(s):  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Age Related

scholarly journals The Pathogenic Role of the Canonical Wnt Pathway in Age-Related Macular Degeneration

2010 ◽  
Vol 51 (9) ◽  
pp. 4371 ◽  
Author(s):  
Ti Zhou ◽  
Yang Hu ◽  
Ying Chen ◽  
Kevin K. Zhou ◽  
Bin Zhang ◽  
...  
Keyword(s):  
Macular Degeneration ◽  
Wnt Pathway ◽  
Age Related Macular Degeneration ◽  
Pathogenic Role ◽  
Canonical Wnt Pathway ◽  
Age Related ◽  
Canonical Wnt

scholarly journals Epigallocatechin-3-gallate Protects against Hydrogen Peroxide-Induced Inhibition of Osteogenic Differentiation of Human Bone Marrow-Derived Mesenchymal Stem Cells

2016 ◽  
Vol 2016 ◽  
pp. 1-10 ◽  
Author(s):  
Dawei Wang ◽  
Yonghui Wang ◽  
Shihong Xu ◽  
Fu Wang ◽  
Bomin Wang ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Adverse Effect ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Differentiation ◽  
Wnt Pathway ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Positive Effects

Oxidative stress induces bone loss and osteoporosis, and epigallocatechin-3-gallate (EGCG) may be used to combat these diseases due to its antioxidative property. Herein, oxidative stress in human bone marrow-derived mesenchymal stem cells (BM-MSCs) was induced by H2O2, resulting in an adverse effect on their osteogenic differentiation. However, this H2O2-induced adverse effect was nullified when the cells were treated with EGCG. In addition, treatment of BM-MSCs with EGCG alone also resulted in the enhancement of osteogenic differentiation of BM-MSCs. After EGCG treatment, expressions of β-catenin and cyclin D1 were upregulated, suggesting that the Wnt pathway was involved in the effects of EGCG on the osteogenic differentiation of BM-MSCs. This was also confirmed by the fact that the Wnt pathway inhibitor, Dickkopf-1 (DKK-1), can nullify the EGCG-induced enhancement effect on BM-MSC’s osteogenic differentiation. Hence, our results suggested that EGCG can reduce the effects of oxidative stress on Wnt pathway in osteogenic cells, which supported a potentially promising therapy of bone disorders induced by oxidative stress. Considering its positive effects on BM-MSCs, EGCG may also be beneficial for stem cell-based bone repair.

Physical activity improves age-related decline in the osteogenic potential of rats' bone marrow-derived mesenchymal stem cells

2012 ◽  
Vol 205 (2) ◽  
pp. 292-301 ◽  
Author(s):  
R. C. R. Hell ◽  
N. M. Ocarino ◽  
J. N. Boeloni ◽  
J. F. Silva ◽  
A. M. Goes ◽  
...  
Keyword(s):  
Physical Activity ◽  
Stem Cells ◽  
Bone Marrow ◽  
Mesenchymal Stem Cells ◽  
Osteogenic Potential ◽  
Age Related
Sign in / Sign up

Export Citation Format

Share Document