scholarly journals Aging differentially modulates the Wnt pro‐survival signalling pathways in vascular smooth muscle cells

Aging Cell ◽  
2018 ◽  
pp. e12844 ◽  
Author(s):  
Bethan A. Brown ◽  
Georgia M. Connolly ◽  
Carina E. J. Mill ◽  
Helen Williams ◽  
Gianni D. Angelini ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Signalling Pathways

PDGF-AB and FGF-2 trigger different signalling pathways in vascular smooth muscle cells

2002 ◽  
Vol 34 (6) ◽  
pp. A68
Author(s):  
Sabina Vogel ◽  
Thomas Kubin ◽  
Elisabeth Deindl ◽  
Matthias Heil ◽  
Wolfgang Schaper ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Signalling Pathways

Intracellur signalling pathways and binding of endothelin in vascular smooth muscle cells

1990 ◽  
Vol 183 (3) ◽  
pp. 676
Author(s):  
P-E. Chabrier ◽  
M-O. Lonchampt ◽  
P. Roubert ◽  
V. Pinelis ◽  
M. Auguet ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Muscle Cells ◽  
Signalling Pathways

Thrombin Receptor Activating Peptide (TRAP) Stimulates Mitogenesis, c-fos and PDGF-A Gene Expression in Human Vascular Smooth Muscle Cells

1995 ◽  
Vol 74 (05) ◽  
pp. 1340-1347 ◽  
Author(s):  
Chryso Kanthou ◽  
Omar Benzakour ◽  
Geeta Patel ◽  
John Deadman ◽  
Vijay Vir Kakkar ◽  
...  
Keyword(s):  
Smooth Muscle ◽  
Vascular Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Vascular Smooth Muscle Cells ◽  
Receptor Occupancy ◽  
Muscle Cells ◽  
Signalling Pathways ◽  
Thrombin Receptor ◽  
Computer Assisted ◽  
Computer Assisted Image

SummaryThe synthetic peptide SFLLRNPNDKYEPF, identical in sequence to the new amino-terminus of the thrombin receptor generated following cleavage by thrombin, acts as a thrombin receptor agonist/ activating peptide (TRAP). In this study, Northern blot analysis showed that cultured human vascular smooth muscle cells (HVSMC) express a thrombin receptor transcript. TRAP, in contrast to thrombin was shown to be a weak mitogen for HVSMC. A combination of TRAP and enzymatically-inactivated thrombin (PPACK-thrombin) which provides receptor occupancy, did not potentiate TRAP-induced mitogenesis, indicating that TRAP and PPACK-thrombin do not reproduce the mitogenic effect of enzymatically-active thrombin. Both thrombin and TRAP, induced the expression of c-fos and the PDGF-A gene in a pertussis toxin (PTX)-insensitive manner. Examination of thrombin and TRAP-treated cells by immunofluorescence staining followed by computer assisted image analysis revealed that thrombin and to a lesser extent TRAP induced PDGF-AA protein expression. Antibodies to PDGF-AA partially inhibited thrombin but not TRAP-induced mitogenesis in HVSMC. This study indicates that in addition to the common signalling pathways utilised by thrombin and TRAP, enzymatically-active thrombin activates other signalling pathways and hence TRAP does not mimic fully the biological effect of thrombin on HVSMC.

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