scholarly journals Local delivery of tetramethylpyrazine eliminates the senescent phenotype of bone marrow mesenchymal stromal cells and creates an anti-inflammatory and angiogenic environment in aging mice

Aging Cell ◽  
2018 ◽  
Vol 17 (3) ◽  
pp. e12741 ◽  
Author(s):  
Bo Gao ◽  
Xisheng Lin ◽  
Huan Jing ◽  
Jing Fan ◽  
Chenchen Ji ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Local Delivery ◽  
Senescent Phenotype ◽  
Anti Inflammatory

Anti-Inflammatory/Tissue Repair Macrophages Enhance the Cartilage-Forming Capacity of Human Bone Marrow-Derived Mesenchymal Stromal Cells

2015 ◽  
Vol 230 (6) ◽  
pp. 1258-1269 ◽  
Author(s):  
Sergio B. Sesia ◽  
Ralph Duhr ◽  
Carolina Medeiros da Cunha ◽  
Atanas Todorov ◽  
Stefan Schaeren ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Tissue Repair ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Anti Inflammatory ◽  
Inflammatory Tissue

scholarly journals Human Bone Marrow Mesenchymal Stromal Cells Attenuate Tissue Injury and Reduce Inflammation in Experimental Acute Pancreatitis

2021 ◽  
Author(s):  
Tayebeh Mahmoudi ◽  
Ali Jalili ◽  
Kamal Abdolmohammadi ◽  
Shohreh Fakhari ◽  
Fatemeh Pahlavan ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Acute Pancreatitis ◽  
Mesenchymal Stromal Cells ◽  
Inflammatory Disease ◽  
Stromal Cells ◽  
Tissue Injury ◽  
Intraperitoneal Administration ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Anti Inflammatory

Purpose: Acute pancreatitis (AP) which is distinguished by local pancreatic necrosis, following by systemic organ failure is known as an inflammatory disease. Up to now, there are only a few treatment options accessible for patients suffering from AP. In this study, we aimed to examine the anti-inflammatory capacities of human bone marrow-derived mesenchymal stromal cells (hBM-MSC) in a detailed AP model experiment. Methods: AP was induced in C57BL/6 mice by intraperitoneal administration of cerulein (100 µg/kg/h × 7 doses) at intervals of 1 hour (h). Then, 2×105 MSCs were infused in the AP mice by tail vein 6 h after the last cerulein injection. Mice were sacrificed 12 h following the injection of hBM-MSC, and blood samples and pancreas tissues were obtained. Results: We first determined the presence of transplanted hBM-MSC in the pancreas of mice with AP, but not the control mice. Our data indicate that administration of hBM-MSCs to mice with AP lead to (i) decreased serum levels of amylase, lipase and myeloperoxidase activities, (ii) downregulation of proinflammatory cytokine, macrophage inflammatory protein 2 (MIP-2), and (iii) upregulation of the anti-inflammatory cytokine, interleukin 10 (IL-10). Moreover, hBM-MSC administration results in notably attenuated cerulein-induced histopathological alternations and edema. Conclusion: we demonstrate that hBM-MSC attenuates AP signs and indicating that hMB-MSC therapy could be a suitable approach for the treatment of inflammatory disease such as AP.

scholarly journals Bone Marrow-Derived Mesenchymal Stromal Cells (MSCs) Modulate the Inflammatory Character of Alveolar Macrophages from Sarcoidosis Patients

2020 ◽  
Vol 9 (1) ◽  
pp. 278 ◽  
Author(s):  
Ian McClain Caldwell ◽  
Christopher Hogden ◽  
Krisztian Nemeth ◽  
Michael Boyajian ◽  
Miklos Krepuska ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Flow Cytometry ◽  
Mesenchymal Stromal Cells ◽  
Alveolar Macrophages ◽  
Stromal Cells ◽  
Cultured Cells ◽  
Intracellular Cytokines ◽  
Tnf Α ◽  
Inflammatory Phenotype ◽  
Anti Inflammatory

Sarcoidosis is a devastating inflammatory disease affecting many organs, especially the lungs and lymph nodes. Bone marrow-derived mesenchymal stromal cells (MSCs) can “reprogram” various types of macrophages towards an anti-inflammatory phenotype. We wanted to determine whether alveolar macrophages from sarcoidosis subjects behave similarly by mounting an anti-inflammatory response when co-cultured with MSCs. Fifteen sarcoidosis and eight control subjects underwent bronchoscopy and bronchoalveolar lavage (BAL). Unselected BAL cells (70–94% macrophages) were isolated and cultured with and without MSCs from healthy adults. Following stimulation of the cultured cells with lipopolysaccharide, the medium was removed to measure interleukin 10 and tumor necrosis factor alpha (IL-10 and TNF-α). In two additional sarcoidosis subjects, flow cytometry was used to study intracellular cytokines and surface markers associated with alveolar macrophages to confirm the results. Unselected BAL cells from sarcoidosis subjects co-cultured with MSCs showed a reduction in TNF-α (pro-inflammatory M1) and an increase in IL-10 (anti-inflammatory M2) in 9 of 11 samples studied. Control subject samples showed few, if any, differences in cytokine production. Unselected BAL cells from two additional patients analyzed by flow cytometry confirmed a switch towards an anti-inflammatory state (i.e., M1 to M2) after co-culture with MSCs. These results suggest that, similarly to other macrophages, alveolar macrophages also respond to MSC contacts by changing towards an anti-inflammatory phenotype. Based on our results, we hypothesize that mesenchymal stromal cells applied to the airways might alleviate lung inflammation and decrease steroid need in patients with sarcoidosis.

scholarly journals Caspase‐8 regulates the expression of pro‐ and anti‐inflammatory cytokines in human bone marrow‐derived mesenchymal stromal cells

2016 ◽  
Vol 4 (3) ◽  
pp. 327-337 ◽  
Author(s):  
Siv H. Moen ◽  
Marita Westhrin ◽  
Muhammad Zahoor ◽  
Nikolai N. Nørgaard ◽  
Hanne Hella ◽  
...  
Keyword(s):  
Bone Marrow ◽  
Inflammatory Cytokines ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Human Bone ◽  
Human Bone Marrow ◽  
Caspase 8 ◽  
Anti Inflammatory

Mechanisms of action and osteogenic activity of bone marrow mesenchymal stromal cells are donor dependent

2014 ◽  
Author(s):  
Julie Leotot ◽  
Angelique Lebouvier ◽  
Philippe Hernigou ◽  
Helene Rouard ◽  
Nathalie Chevallier
Keyword(s):  
Bone Marrow ◽  
Mesenchymal Stromal Cells ◽  
Stromal Cells ◽  
Mechanisms Of Action ◽  
Osteogenic Activity
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