scholarly journals The amino acid transporter SLC36A4 regulates the amino acid pool in retinal pigmented epithelial cells and mediates the mechanistic target of rapamycin, complex 1 signaling

Aging Cell ◽  
2017 ◽  
Vol 16 (2) ◽  
pp. 349-359 ◽  
Author(s):  
Peng Shang ◽  
Mallika Valapala ◽  
Rhonda Grebe ◽  
Stacey Hose ◽  
Sayan Ghosh ◽  
...  
Keyword(s):  
Amino Acid ◽  
Epithelial Cells ◽  
Amino Acid Transporter ◽  
Target Of Rapamycin ◽  
Amino Acid Pool ◽  
Acid Pool ◽  
Mechanistic Target Of Rapamycin ◽  
Retinal Pigmented Epithelial Cells ◽  
Acid Transporter

Deletion of the amino acid transporter Slc6a14 suppresses tumour growth in spontaneous mouse models of breast cancer

2015 ◽  
Vol 469 (1) ◽  
pp. 17-23 ◽  
Author(s):  
Ellappan Babu ◽  
Yangzom D. Bhutia ◽  
Sabarish Ramachandran ◽  
Jaya P. Gnanaprakasam ◽  
Puttur D. Prasad ◽  
...  
Keyword(s):  
Breast Cancer ◽  
Amino Acid ◽  
Mouse Models ◽  
Therapeutic Target ◽  
Tumour Growth ◽  
Mammalian Target Of Rapamycin ◽  
Amino Acid Transporter ◽  
Mtor Pathway ◽  
Target Of Rapamycin ◽  
Acid Transporter

Deletion of the amino acid transporter Slc6a14 in mice suppresses tumour growth in spontaneous models of breast cancer via interference with mammalian target of rapamycin (mTOR) pathway; this indicates an obligatory role for SLC6A14 in breast cancer, highlighting its potential as a therapeutic target.

192 AMINO ACID TRANSPORTER EXPRESSION IN BOVINE OVIDUCT EPITHELIAL CELLS

2008 ◽  
Vol 20 (1) ◽  
pp. 175
Author(s):  
S. L. Whitear ◽  
H. J. Leese
Keyword(s):  
Amino Acid ◽  
Epithelial Cells ◽  
Amino Acid Transporter ◽  
Positive Control ◽  
Amino Acid Transporters ◽  
Oviduct Epithelial Cells ◽  
Acid Transporter ◽  
Oviduct Fluid ◽  
Transporter Expression

Oviduct fluid provides the environment for the gametes and early embryo but little is known about the mechanisms underlying its formation. Components of oviduct fluid have been shown to be present at concentrations different from that in blood, indicative of selective transport by the epithelial cells lining the lumen. For example, amino acid concentrations in oviduct fluid differ from those in extracellular fluid and have also been shown to be important to preimplantation embryos in vitro, enhancing development, especially when added at physiological concentrations. However, little is known about amino acid transport systems in the oviduct, and the aim of this work was to search for mRNA transcripts for amino acid transporters in bovine oviduct epithelial cells. Contra- and ipsi-lateral oviducts were removed from abattoir-derived reproductive tracts at specific stages of the reproductive cycle. Oviducts were trimmed of surrounding tissue and fat and slit longitudinally to expose the luminal surface. Bovine oviduct epithelial cells (bOEC) were scraped from the surface using a sterile glass coverslip and washed by centrifugation. mRNA was isolated using Trizol-chloroform extraction and lithium chloride precipitation methods. PCR was used to detect cDNA encoding the amino acid transporters CAT-1, CAT-4, and LAT1. A negative control (water) and a positive control (human placental cDNA) were included in each experiment and β-actin expression was used as a positive control for cDNA library generation. Products were separated by agarose gel electrophoresis. PCR for β-actin resulted in the presence of a positive band in all samples, showing successful extraction of mRNA and generation of cDNA libraries. mRNA for CAT-1 and LAT1 was detected in bOEC from contra- and ipsi-lateral oviducts and from each cycle stage tested. There was, however, no detectable mRNA for CAT-4 in any of the samples. To our knowledge, this is the first report of amino acid transporter expression in the mammalian oviduct. CAT-1 is a ubiquitous sodium-independent uniporter of cationic amino acids that has been localized to the basolateral membrane of epithelial cells. The presence of mRNA for this amino acid transporter in all samples tested is therefore to be expected. LAT1 is a obligatory exchanger which exports glutamine and cystine and imports large uncharged branched-chain amino acids. This transporter may be partly responsible for the high concentration of glutamate in the basal compartment of in vitro cell cultures reported in our previous work (Whitear and Leese 2007 Biennial Meet. Joint Fertil. Soc., York, UK). CAT-4 shares only 40% sequence homology with CAT-1 and its function is unknown. Its expression appears to be restricted to brain, testis, and placenta, and the absence of mRNA for the oviduct was, perhaps, not surprising. Further experiments will investigate expression levels of other amino acid transporters in bOEC and transporter localization using immunohistochemistry. This work was funded by the BBSRC and ANGLE Technology Ltd.

scholarly journals Increased ubiquitination and reduced plasma membrane trafficking of placental amino acid transporter SNAT-2 in human IUGR

2015 ◽  
Vol 129 (12) ◽  
pp. 1131-1141 ◽  
Author(s):  
Yi-Yung Chen ◽  
Fredrick J. Rosario ◽  
Majida Abu Shehab ◽  
Theresa L. Powell ◽  
Madhulika B. Gupta ◽  
...  
Keyword(s):  
Plasma Membrane ◽  
Amino Acid ◽  
Ubiquitin Ligase ◽  
Membrane Trafficking ◽  
Amino Acid Transporter ◽  
Acid Transport ◽  
Mechanistic Target Of Rapamycin ◽  
Neutral Amino Acid Transporter ◽  
Acid Transporter ◽  
Mtor Signalling

Inhibition of placental mechanistic target of rapamycin (mTOR) signalling, which activates NEDD4-2 (neural precursor cell expressed developmentally down-regulated protein 4-2) ubiquitin ligase leading to increased sodium-coupled neutral amino acid transporter 2 (SNAT-2) ubiquitination and removal from the syncytiotrophoblast plasma membrane may constitute a key mechanism underlying decreased placental amino acid transport in human IUGR.

scholarly journals Hyperoxia Inhibits Nitric Oxide Treatment Effects in Alveolar Epithelial Cells via Effects on L-Type Amino Acid Transporter-1

2014 ◽  
Vol 21 (13) ◽  
pp. 1823-1836 ◽  
Author(s):  
Mulugu V. Brahmajothi ◽  
Brian T. Tinch ◽  
Michael F. Wempe ◽  
Hitoshi Endou ◽  
Richard L. Auten
Keyword(s):  
Nitric Oxide ◽  
Amino Acid ◽  
Epithelial Cells ◽  
Treatment Effects ◽  
Amino Acid Transporter ◽  
Alveolar Epithelial Cells ◽  
Alveolar Epithelial ◽  
Acid Transporter
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