scholarly journals Dimethyl sulfide protects against oxidative stress and extends lifespan via a methionine sulfoxide reductase A-dependent catalytic mechanism

Aging Cell ◽  
2016 ◽  
Vol 16 (2) ◽  
pp. 226-236 ◽  
Author(s):  
Xin-Lei Guan ◽  
Peng-Fei Wu ◽  
Sheng Wang ◽  
Juan-Juan Zhang ◽  
Zu-Cheng Shen ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Dimethyl Sulfide ◽  
Catalytic Mechanism ◽  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Sulfoxide Reductase A

scholarly journals An unusual thioredoxin system in the facultative parasite Acanthamoeba castellanii

2021 ◽  
Vol 78 (7) ◽  
pp. 3673-3689
Author(s):  
David Leitsch ◽  
Alvie Loufouma Mbouaka ◽  
Martina Köhsler ◽  
Norbert Müller ◽  
Julia Walochnik
Keyword(s):  
Oxidative Stress ◽  
Stop Codon ◽  
Acanthamoeba Castellanii ◽  
Methionine Sulfoxide ◽  
Redox System ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Sulfoxide Reductase A ◽  
Protein Levels ◽  
Thioredoxin System ◽  
Facultative Parasite

AbstractThe free-living amoeba Acanthamoeba castellanii occurs worldwide in soil and water and feeds on bacteria and other microorganisms. It is, however, also a facultative parasite and can cause serious infections in humans. The annotated genome of A. castellanii (strain Neff) suggests the presence of two different thioredoxin reductases (TrxR), of which one is of the small bacterial type and the other of the large vertebrate type. This combination is highly unusual. Similar to vertebrate TrxRases, the gene coding for the large TrxR in A. castellanii contains a UGA stop codon at the C-terminal active site, suggesting the presence of selenocysteine. We characterized the thioredoxin system in A. castellanii in conjunction with glutathione reductase (GR), to obtain a more complete understanding of the redox system in A. castellanii and the roles of its components in the response to oxidative stress. Both TrxRases localize to the cytoplasm, whereas GR localizes to the cytoplasm and the large organelle fraction. We could only identify one thioredoxin (Trx-1) to be indeed reduced by one of the TrxRases, i.e., by the small TrxR. This thioredoxin, in turn, could reduce one of the two peroxiredoxins tested and also methionine sulfoxide reductase A (MsrA). Upon exposure to hydrogen peroxide and diamide, only the small TrxR was upregulated in expression at the mRNA and protein levels, but not the large TrxR. Our results show that the small TrxR is involved in the A. castellanii’s response to oxidative stress. The role of the large TrxR, however, remains elusive.

scholarly journals Methionine sulfoxide reductase B from Corynebacterium diphtheriae catalyzes sulfoxide reduction via an intramolecular disulfide cascade

2020 ◽  
Vol 295 (11) ◽  
pp. 3664-3677
Author(s):  
Maria-Armineh Tossounian ◽  
Anh-Co Khanh Truong ◽  
Lieven Buts ◽  
Khadija Wahni ◽  
Álvaro Mourenza ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Quantum Coherence ◽  
Solution Structure ◽  
Structural Changes ◽  
Catalytic Mechanism ◽  
Methionine Sulfoxide ◽  
Structural Rearrangement ◽  
Corynebacterium Diphtheriae ◽  
Methionine Sulfoxide Reductase ◽  
Bacterial Survival

Corynebacterium diphtheriae is a human pathogen that causes diphtheria. In response to immune system-induced oxidative stress, C. diphtheriae expresses antioxidant enzymes, among which are methionine sulfoxide reductase (Msr) enzymes, which are critical for bacterial survival in the face of oxidative stress. Although some aspects of the catalytic mechanism of the Msr enzymes have been reported, several details still await full elucidation. Here, we solved the solution structure of C. diphtheriae MsrB (Cd-MsrB) and unraveled its catalytic and oxidation-protection mechanisms. Cd-MsrB catalyzes methionine sulfoxide reduction involving three redox-active cysteines. Using NMR heteronuclear single-quantum coherence spectra, kinetics, biochemical assays, and MS analyses, we show that the conserved nucleophilic residue Cys-122 is S-sulfenylated after substrate reduction, which is then resolved by a conserved cysteine, Cys-66, or by the nonconserved residue Cys-127. We noted that the overall structural changes during the disulfide cascade expose the Cys-122–Cys-66 disulfide to recycling through thioredoxin. In the presence of hydrogen peroxide, Cd-MsrB formed reversible intra- and intermolecular disulfides without losing its Cys-coordinated Zn2+, and only the nonconserved Cys-127 reacted with the low-molecular-weight (LMW) thiol mycothiol, protecting it from overoxidation. In summary, our structure-function analyses reveal critical details of the Cd-MsrB catalytic mechanism, including a major structural rearrangement that primes the Cys-122–Cys-66 disulfide for thioredoxin reduction and a reversible protection against excessive oxidation of the catalytic cysteines in Cd-MsrB through intra- and intermolecular disulfide formation and S-mycothiolation.

scholarly journals Gene structure, localization and role in oxidative stress of methionine sulfoxide reductase A (MSRA) in the monkey retina

2006 ◽  
Vol 82 (5) ◽  
pp. 816-827 ◽  
Author(s):  
J.W. Lee ◽  
N.V. Gordiyenko ◽  
M. Marchetti ◽  
N. Tserentsoodol ◽  
D. Sagher ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Gene Structure ◽  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Sulfoxide Reductase A

A Structural Analysis of the Catalytic Mechanism of Methionine Sulfoxide Reductase A from Neisseria meningitidis

2008 ◽  
Vol 377 (1) ◽  
pp. 268-280 ◽  
Author(s):  
Fanomezana M. Ranaivoson ◽  
Mathias Antoine ◽  
Brice Kauffmann ◽  
Sandrine Boschi-Muller ◽  
André Aubry ◽  
...  
Keyword(s):  
Structural Analysis ◽  
Neisseria Meningitidis ◽  
Catalytic Mechanism ◽  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Sulfoxide Reductase A

scholarly journals Methionine sulfoxide reductase A is important for lens cell viability and resistance to oxidative stress

2004 ◽  
Vol 101 (26) ◽  
pp. 9654-9659 ◽  
Author(s):  
M. Kantorow ◽  
J. R. Hawse ◽  
T. L. Cowell ◽  
S. Benhamed ◽  
G. O. Pizarro ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Cell Viability ◽  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Sulfoxide Reductase A ◽  
Lens Cell

Methionine Sulfoxide Reductase A, B1 and B2 Are Likely to Be Involved in the Protection against Oxidative Stress in the Inner Ear

2014 ◽  
Vol 199 (4) ◽  
pp. 294-300 ◽  
Author(s):  
Tae-Jun Kwon ◽  
Se-Kyung Oh ◽  
Ye-Ri Kim ◽  
Min-A Kim ◽  
Byeonghyeon Lee ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Inner Ear ◽  
Methionine Sulfoxide ◽  
Methionine Sulfoxide Reductase ◽  
Methionine Sulfoxide Reductase A
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