scholarly journals Blockade of senescence‐associated micro RNA ‐195 in aged skeletal muscle cells facilitates reprogramming to produce induced pluripotent stem cells.

Aging Cell ◽  
2015 ◽  
Vol 15 (1) ◽  
pp. 56-66 ◽  
Author(s):  
Hideyuki Kondo ◽  
Ha Won Kim ◽  
Lei Wang ◽  
Motoi Okada ◽  
Christian Paul ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Muscle Cells ◽  
Micro Rna ◽  
Skeletal Muscle Cells ◽  
Induced Pluripotent

scholarly journals Induction of Skeletal Muscle Progenitors and Stem Cells from human induced Pluripotent Stem Cells

2020 ◽  
Vol 7 (4) ◽  
pp. 395-405
Author(s):  
Takahiko Sato
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Myogenic Differentiation ◽  
Muscle Cells ◽  
Skeletal Muscle Cells ◽  
Induced Pluripotent ◽  
Myogenic Induction

Induced pluripotent stem cells (iPSCs) have the potential to differentiate into various types of cells and tissues including skeletal muscle. The approach to convert these stem cells into skeletal muscle cells offers hope for patients afflicted with skeletal muscle diseases such as Duchenne muscular dystrophy (DMD). Several methods have been reported to induce myogenic differentiation with iPSCs derived from myogenic patients. An important point for generating skeletal muscle cells from iPSCs is to understand in vivo myogenic induction in development and regeneration. Current protocols of myogenic induction utilize techniques with overexpression of myogenic transcription factors such as Myod1(MyoD), Pax3, Pax7, and others, using recombinant proteins or small molecules to induce mesodermal cells followed by myogenic progenitors, and adult muscle stem cells. This review summarizes the current approaches used for myogenic induction and highlights recent improvements.

scholarly journals Genetic Rescue of Mitochondrial and Skeletal Muscle Impairment in an Induced Pluripotent Stem Cells Model of Coenzyme Q10 Deficiency

Stem Cells ◽  
2017 ◽  
Vol 35 (7) ◽  
pp. 1687-1703 ◽  
Author(s):  
Damià Romero-Moya ◽  
Carlos Santos-Ocaña ◽  
Julio Castaño ◽  
Gloria Garrabou ◽  
José A. Rodríguez-Gómez ◽  
...  
Keyword(s):  
Skeletal Muscle ◽  
Stem Cells ◽  
Induced Pluripotent Stem Cells ◽  
Coenzyme Q10 ◽  
Pluripotent Stem Cells ◽  
Genetic Rescue ◽  
Induced Pluripotent ◽  
Muscle Impairment

Abstract 13982: Derivation of Functional Smooth Muscle Cells From Integration-free Induced Pluripotent Stem Cells Through Cardiovascular Progenitor Cell Intermediates for Vascular Regeneration

Circulation ◽  
2014 ◽  
Vol 130 (suppl_2) ◽  
Author(s):  
Yongyu Wang ◽  
Jiang Hu ◽  
Jiao jiao ◽  
Zhou Zhou ◽  
Eugene Y Chen ◽  
...  
Keyword(s):  
Gene Expression ◽  
Stem Cells ◽  
Smooth Muscle ◽  
Smooth Muscle Cells ◽  
Induced Pluripotent Stem Cells ◽  
Pluripotent Stem Cells ◽  
Progenitor Cell ◽  
Muscle Cells ◽  
Vascular Regeneration ◽  
Induced Pluripotent

Tissue-engineered blood vessels (TEBVs) hold great promise for replacement of damaged or defective vascular tissues in vascular disease therapies, such as coronary and peripheral bypass graft surgeries. However, it remains a great challenge to obtain sufficient numbers of functional smooth muscle cells (SMCs) in the practice of constructing patient-specific TEBVs. This study aimed to develop an efficient method to generate a large number of functional SMCs in a short term for constructing tissue-engineered vascular tissues. Human induced pluripotent stem cells (iPSCs) were established by integration-free episomal vector-based reprogramming of donor peripheral blood mononuclear cells (PBMCs). These established iPSCs expressed pluripotency markers and were demonstrated to be able to differentiate into all three germ layer cells. Cardiovascular progenitor cell (CVPC) intermediates were then promptly and efficiently induced and expanded in chemically defined medium. Vascular smooth muscle cells (SMCs) were further induced under differentiation condition, which expressed typical SMCs markers including smooth muscle α-actin (α-SMA), calponin and SM22α validated by quantitative real-time PCR and immunocytochemistry stain. Importantly, the derived SMCs showed functional properties, validated by contraction responsiveness to carbachol treatment, up-regulation of specific collagens gene expression under transforming growth factor β1 treatment and up-regulation of specific matrix metalloproteinases gene expression under cytokine stimuli. Future studies will be focused on using these functional SMCs to construct functional TEBVs on biomimetic scaffolds. Taken together, our study established a facile procedure to generate large amount of functional and safe SMCs for vascular regeneration, via establishment of donor-specific integration-free human iPSCs and directed differentiation through CVPC intermediates.

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