scholarly journals Rifampicin reduces advanced glycation end products and activates DAF ‐16 to increase lifespan in Caenorhabditis elegans

Aging Cell ◽  
2015 ◽  
Vol 14 (3) ◽  
pp. 463-473 ◽  
Author(s):  
Sandeep Golegaonkar ◽  
Syed S. Tabrez ◽  
Awadhesh Pandit ◽  
Shalini Sethurathinam ◽  
Mashanipalya G. Jagadeeshaprasad ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Advanced Glycation End Products ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products

scholarly journals Exposure of Caenorhabditis elegans to Dietary Nε-Carboxymethyllysine Emphasizes Endocytosis as a New Route for Intestinal Absorption of Advanced Glycation End Products

Nutrients ◽  
2021 ◽  
Vol 13 (12) ◽  
pp. 4398
Author(s):  
Constance Dubois ◽  
Rachel Litke ◽  
Stéphane Rianha ◽  
Charles Paul-Constant ◽  
Jean-Marc Lo Guidice ◽  
...  
Keyword(s):  
Caenorhabditis Elegans ◽  
Intestinal Absorption ◽  
Advanced Glycation End Products ◽  
Peptide Transporter ◽  
Advanced Glycation ◽  
Pathophysiological Mechanisms ◽  
Glycation End Products ◽  
End Products ◽  
The Impact ◽  
Absorption Pathways

The impact of dietary advanced glycation end products (dAGEs) on human health has been discussed in many studies but, to date, no consensual pathophysiological process has been demonstrated. The intestinal absorption pathways which have so far been described for dAGEs, the passive diffusion of free AGE adducts and transport of glycated di-tripeptides by the peptide transporter 1 (PEPT-1), are not compatible with certain pathophysiological processes described. To get new insight into the intestinal absorption pathways and the pathophysiological mechanisms of dAGEs, we initiated an in vivo study with a so-called simple animal model with a complete digestive tract, Caenorhabditis elegans. Dietary bacteria were chemically modified with glyoxylic acid to mainly produce Nε-carboxymethyllysine (CML) and used to feed the worms. We performed different immunotechniques using an anti-CML antibody for the relative quantification of ingested CML and localization of this AGE in the worms’ intestine. The relative expression of genes encoding different biological processes such as response to stresses and intestinal digestion were determined. The physiological development of the worms was verified. All the results were compared with those obtained with the control bacteria. The results revealed a new route for the intestinal absorption of dietary CML (dCML), endocytosis, which could be mediated by scavenger receptors. The exposure of worms to dCML induced a reproductive defect and a transcriptional response reflecting oxidative, carbonyl and protein folding stresses. These data, in particular the demonstration of endocytosis of dCML by enterocytes, open up new perspectives to better characterize the pathophysiological mechanisms of dAGEs.

scholarly journals Autofluorescence as a noninvasive biomarker of senescence and advanced glycation end products in Caenorhabditis elegans

2021 ◽  
Vol 7 (1) ◽  
Author(s):  
Tomomi Komura ◽  
Mikihiro Yamanaka ◽  
Kohji Nishimura ◽  
Keita Hara ◽  
Yoshikazu Nishikawa
Keyword(s):  
Caenorhabditis Elegans ◽  
Advanced Glycation End Products ◽  
Fluorescence Intensity ◽  
High Resolution Mass Spectrometry ◽  
Age Factors ◽  
Advanced Glycation ◽  
Glycation End Products ◽  
End Products ◽  
Noninvasive Biomarker

AbstractTo assess the utility of autofluorescence as a noninvasive biomarker of senescence in Caenorhabditis elegans, we measured the autofluorescence of individual nematodes using spectrofluorometry. The fluorescence of each worm increased with age. Animals with lower fluorescence intensity exhibited longer life expectancy. When proteins extracted from worms were incubated with sugars, the fluorescence intensity and the concentration of advanced glycation end products (AGEs) increased over time. Ribose enhanced these changes not only in vitro but also in vivo. The glycation blocker rifampicin suppressed this rise in fluorescence. High-resolution mass spectrometry revealed that vitellogenins accumulated in old worms, and glycated vitellogenins emitted six-fold higher fluorescence than naive vitellogenins. The increase in fluorescence with ageing originates from glycated substances, and therefore could serve as a useful noninvasive biomarker of AGEs. C. elegans can serve as a new model to look for anti-AGE factors and to study the relationship between AGEs and senescence.

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