scholarly journals Large chromosome deletions, duplications, and gene conversion events accumulate with age in normal human colon crypts

Aging Cell ◽  
2013 ◽  
Vol 12 (2) ◽  
pp. 269-279 ◽  
Author(s):  
John C. F. Hsieh ◽  
David Van Den Berg ◽  
Haeyoun Kang ◽  
Chih-Lin Hsieh ◽  
Michael R. Lieber
Keyword(s):  
Gene Conversion ◽  
Human Colon ◽  
Large Chromosome ◽  
Normal Human ◽  
Chromosome Deletions

The influence of extracts from Potentilla species on normal human colon cells

Planta Medica ◽  
2011 ◽  
Vol 77 (12) ◽  
Author(s):  
R Paduch ◽  
M Tomczyk ◽  
A Wiater ◽  
M Pleszczynska ◽  
M Kandefer Szerszen ◽  
...  
Keyword(s):  
Human Colon ◽  
Colon Cells ◽  
Normal Human

Normal human colon epithelial cells propagated in long-term cell cultures support JC polyomavirus infection

2001 ◽  
Vol 33 ◽  
pp. A40
Author(s):  
Farhad F. Shadan ◽  
Luigi Ricciardiello ◽  
Ajay Goel ◽  
Wendy Smith ◽  
Dong K. Chang ◽  
...  
Keyword(s):  
Epithelial Cells ◽  
Cell Cultures ◽  
Human Colon ◽  
Jc Polyomavirus ◽  
Normal Human

scholarly journals Colonic Saturated Fatty Acid Concentrations and Expression of COX-1, but not Diet, Predict Prostaglandin E2in Normal Human Colon Tissue

2016 ◽  
Vol 68 (7) ◽  
pp. 1192-1201 ◽  
Author(s):  
ElKhansa Sidahmed ◽  
Ananda Sen ◽  
Jianwei Ren ◽  
Arsh Patel ◽  
D. Kim Turgeon ◽  
...  
Keyword(s):  
Fatty Acid ◽  
Saturated Fatty Acid ◽  
Human Colon ◽  
Colon Tissue ◽  
Normal Human ◽  
Cox 1

Organoid cultures of early-onset colorectal cancers reveal distinct and rare genetic profiles

Gut ◽  
2020 ◽  
Vol 69 (12) ◽  
pp. 2165-2179 ◽  
Author(s):  
Helen H N Yan ◽  
Hoi Cheong Siu ◽  
Siu Lun Ho ◽  
Sarah S K Yue ◽  
Yang Gao ◽  
...  
Keyword(s):  
Transcriptome Analysis ◽  
Early Onset ◽  
Human Colon ◽  
Normal Colon ◽  
Normal Human ◽  
Cell Transcriptome ◽  
Molecular Phenotypes ◽  
Single Cell Transcriptome ◽  
Genetic Profiles ◽  
Early Onset Colorectal Cancer

ObjectiveSporadic early-onset colorectal cancer (EOCRC) has bad prognosis, yet is poorly represented by cell line models. We examine the key mutational and transcriptomic alterations in an organoid biobank enriched in EOCRCs.DesignWe established paired cancer (n=32) and normal organoids (n=18) from 20 patients enriched in microsatellite-stable EOCRC. Exome and transcriptome analysis was performed.ResultsWe observed a striking diversity of molecular phenotypes, including PTPRK-RSPO3 fusions. Transcriptionally, RSPO fusion organoids resembled normal colon organoids and were distinct from APC mutant organoids, with high BMP2 and low PTK7 expression. Single cell transcriptome analysis confirmed the similarity between RSPO fusion organoids and normal organoids, with a propensity for maturation on Wnt withdrawal, whereas the APC mutant organoids were locked in progenitor stages. CRISPR/Cas9 engineered mutation of APC in normal human colon organoids led to upregulation of PTK7 protein and suppression of BMP2, but less so with an engineered RNF43 mutation. The frequent co-occurrence of RSPO fusions with SMAD4 or BMPR1A mutation was confirmed in TCGA database searches. RNF43 mutation was found in organoid from a leukaemia survivor with a novel mutational signature; and organoids with POLE proofreading mutation displayed ultramutation. The cancer organoid genomes were stable over long culture periods, while normal human colon organoids tended to be subject to clonal dominance over time.ConclusionsThese organoid models enriched in EOCRCs with linked genomic data fill a gap in existing CRC models and reveal distinct genetic profiles and novel pathway cooperativity.

Abstract 657: The comparison of calcium-induced differentiation in colonoid cultures derived from the normal human colon tissue and colonic adenomas

2019 ◽  
Author(s):  
Muhammad N. Aslam ◽  
Shannon D. McClintock ◽  
Areeba H. Rizvi ◽  
Durga Attili ◽  
Shailja Pandya ◽  
...  
Keyword(s):  
Human Colon ◽  
Colon Tissue ◽  
Induced Differentiation ◽  
Colonic Adenomas ◽  
Normal Human

scholarly journals Zinc Phthalocyanine Photochemistry by Raman Imaging, Fluorescence Spectroscopy and Femtosecond Spectroscopy in Normal and Cancerous Human Colon Tissues and Single Cells

Molecules ◽  
2020 ◽  
Vol 25 (11) ◽  
pp. 2688 ◽  
Author(s):  
Beata Brozek-Pluska ◽  
Arkadiusz Jarota ◽  
Rafal Kania ◽  
Halina Abramczyk
Keyword(s):  
Single Cells ◽  
Blue Shift ◽  
Human Colon ◽  
Raman Imaging ◽  
Zinc Phthalocyanine ◽  
Label Free ◽  
Colon Tissue ◽  
Tissue Samples ◽  
Colon Cells ◽  
Normal Human

Photodynamic therapy is a clinically approved alternative method for cancer treatment in which a combination of nontoxic drugs known as photosensitizers and oxygen is used. Despite intensive investigations and encouraging results, zinc phthalocyanines (ZnPcs) have not yet been approved as photosensitizers for clinical use. Label-free Raman imaging of nonfixed and unstained normal and cancerous colon human tissues and normal human CCD18-Co and cancerous CaCo-2 cell lines, without and after adding ZnPcS4 photosensitizer, was analyzed. The biochemical composition of normal and cancerous colon tissues and colon cells without and after adding ZnPcS4 at the subcellular level was determined. Analyzing the fluorescence/Raman signals of ZnPcS4, we found that in normal human colon tissue samples, in contrast to cancerous ones, there is a lower affinity to ZnPcS4 phthalocyanine. Moreover, a higher concentration in cancerous tissue was concomitant with a blue shift of the maximum peak position specific for the photosensitizer from 691–695 nm to 689 nm. Simultaneously for both types of samples, the signal was observed in the monomer region, confirming the excellent properties of ZnPcS4 for photo therapy (PDT). For colon cell experiments with a lower concentration of ZnPcS4 photosensitizer, c = 1 × 10−6 M, the phthalocyanine was localized in mitochondria/lipid structures; for a higher concentration, c = 9 × 10−6 M, localization inside the nucleus was predominant. Based on time-resolved experiments, we found that ZnPcS4 in the presence of biological interfaces features longer excited-state lifetime photosensitizers compared to the aqueous solution and bare ZnPcS4 film on CaF2 substrate, which is beneficial for application in PDT.

NCM460, a normal human colon mucosal epithelial cell line

1996 ◽  
Vol 32 (6) ◽  
pp. 315-317 ◽  
Author(s):  
Mary Pat Moyer ◽  
Lawrence A. Manzano ◽  
Ronald L. Merriman ◽  
Jay S. Stauffer ◽  
Lee R. Tanzer
Keyword(s):  
Epithelial Cell ◽  
Cell Line ◽  
Human Colon ◽  
Epithelial Cell Line ◽  
Mucosal Epithelial Cell ◽  
Normal Human

scholarly journals Anti-glycation and anti-oxidative effects of a phenolic-enriched maple syrup extract and its protective effects on normal human colon cells

2017 ◽  
Vol 8 (2) ◽  
pp. 757-766 ◽  
Author(s):  
Weixi Liu ◽  
Zhengxi Wei ◽  
Hang Ma ◽  
Ang Cai ◽  
Yongqiang Liu ◽  
...  
Keyword(s):  
Oxidative Stress ◽  
Human Colon ◽  
Protective Effects ◽  
Colon Cells ◽  
Maple Syrup ◽  
Normal Human ◽  
Oxidative Effects

Phenolic-enriched maple syrup extract (MSX) inhibits the formation of AGEs and protects normal/non-tumorigenic human colon cells from oxidative stress.

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