Treatment with the sphingosine-1-phosphate analogue FTY 720 reduces loss of plasma volume during experimental sepsis in the rat

CORNELIA LUNDBLAD; HELEN AXELBERG; PER-OLOF GRÄNDE

doi:10.1111/aas.12130

Treatment with the sphingosine-1-phosphate analogue FTY 720 reduces loss of plasma volume during experimental sepsis in the rat

Acta Anaesthesiologica Scandinavica ◽

10.1111/aas.12130 ◽

2013 ◽

Vol 57 (6) ◽

pp. 713-718 ◽

Cited By ~ 18

Author(s):

CORNELIA LUNDBLAD ◽

HELEN AXELBERG ◽

PER-OLOF GRÄNDE

Keyword(s):

Plasma Volume ◽

Experimental Sepsis ◽

Fty 720 ◽

Sphingosine 1 Phosphate

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Selective sphingosine 1-phosphate 1 receptor activation reduces ischemia-reperfusion injury in mouse kidney

AJP Renal Physiology ◽

10.1152/ajprenal.00311.2005 ◽

2006 ◽

Vol 290 (6) ◽

pp. F1516-F1524 ◽

Cited By ~ 155

Author(s):

Alaa S. Awad ◽

Hong Ye ◽

Liping Huang ◽

Li Li ◽

Frank W. Foss ◽

...

Keyword(s):

Protective Effect ◽

Dose Response ◽

Ischemia Reperfusion Injury ◽

Ischemia Reperfusion ◽

Receptor Activation ◽

Plasma Creatinine ◽

Leukocyte Infiltration ◽

Fty 720 ◽

Sphingosine 1 Phosphate ◽

Maximal Reduction

The mechanisms involved in renal ischemia-reperfusion injury (IRI) are complex and appear to involve the early participation of bone marrow-derived cells. T lymphocytes participate in the pathogenesis of IRI. Sphingosine 1-phosphate (S1P) induces peripheral T cell depletion. Therefore, we hypothesized that S1P1 receptor activation protects kidney from IRI. FTY-720, a non-receptor-selective sphingosine analog, was given intraperitoneally to C57BL/6 mice, and animals were subjected to ischemia for 32 min followed by reperfusion for 24 h. Plasma creatinine, blood count, myeloperoxidase (MPO) activity, and renal histology were determined. IRI led to a marked increase in plasma creatinine, MPO activity, leukocyte infiltration, and vascular permeability. FTY-720 significantly decreased plasma creatinine in a dose-response manner with a maximal reduction of ∼73 and ∼69% with doses of 240 and 48 μg/kg, respectively. MPO, leukocyte infiltration, vascular permeability, and peripheral blood lymphocyte counts were markedly decreased with FTY-720 treatment. The protective effect of FTY-720 was reversed with VPC-44116, a selective S1P1 receptor antagonist. Furthermore, SEW-2871, a selective S1P1 agonist, significantly decreased plasma creatinine in a dose-response manner with a maximal reduction of ∼70% with a dose of 10 mg/kg. Analysis of kidneys by light microscopy revealed minimal histological signs of ischemic injury with FTY-720 or SEW-2871 treatment compared with the vehicle group. Using RT-PCR, we found a time-dependent increase in the S1P1 mRNA expression following IRI that begins after 2 h with the maximum expression at ∼4 h. We conclude that the protective effect of FTY-720 is due primarily to activation of S1P1 receptors. The mechanism of protection is not known but may be related to peripheral lymphocyte depletion or direct effects on kidney cells expressing S1P1 receptor.

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