Development of mycoinsecticide formulations with Beauveria bassiana and Metarhizium brunneum for the control of Orosanga japonica (Hemiptera: Ricaniidae)

2021 ◽  
Author(s):  
Seda Biryol ◽  
Ebru Güney ◽  
Ardahan Eski ◽  
Zeynep Bayramoğlu ◽  
Kazım Sezen ◽  
...  
Keyword(s):  
Beauveria Bassiana ◽  
Metarhizium Brunneum

The entomopathogenic fungi Metarhizium brunneum and Beauveria bassiana promote systemic immunity and confer resistance to a broad range of pests and pathogens in tomato

2021 ◽  
Author(s):  
Rupali Gupta ◽  
Ravindran Keppanan ◽  
Meirav Leibman-Markus ◽  
Dalia Rav David ◽  
Yigal Elad ◽  
...  
Keyword(s):  
Disease Resistance ◽  
Plant Growth ◽  
Beauveria Bassiana ◽  
Entomopathogenic Fungi ◽  
Plant Pathogens ◽  
Plant Immunity ◽  
Systemic Immunity ◽  
Metarhizium Brunneum ◽  
Promote Plant Growth ◽  
Arthropod Pests

Biocontrol agents can control pathogens by re-enforcing systemic plant resistance through systemic acquired resistance (SAR) or induced systemic resistance (ISR). Trichoderma spp. can activate the plant immune system through ISR, priming molecular mechanisms of defense against pathogens. Entomopathogenic fungi (EPF) can infect a wide range of arthropod pests, and play an important role in reducing pests' population. Here, we investigated the mechanisms by which EPF control plant diseases. We tested two well studied EPF, Metarhizium brunneum isolate Mb7 and Beauveria bassiana as the commercial product Velifer, for their ability to induce systemic immunity and disease resistance against several fungal and bacterial phytopathogens, and their ability to promote plant growth. We compared the activity of these EPF to an established biocontrol agent, T. harzianum T39, a known inducer of systemic plant immunity and broad disease resistance. The three fungal agents were effective against several fungal and bacterial plant pathogens and arthropod pests. Our results indicate that EPF induce systemic plant immunity and disease resistance by activating the plant host defense machinery, as evidenced by increases in reactive oxygen species (ROS) production and defense gene expression, and that EPF promote plant growth. EPF should be considered as control means for Tuta absoluta. We demonstrate that, with some exceptions, biocontrol in tomato can be equally potent by the tested EPF and T. harzianum T39, against both insect pests and plant pathogens. Taken together, our findings suggest that EPF may find use in broad-spectrum pest and disease management and as plant growth promoting agents.

scholarly journals Preventative Approach to Microbial Control of Capnodis tenebrionis by Soil Application of Metarhizium brunneum and Beauveria bassiana

Insects ◽  
2020 ◽  
Vol 11 (5) ◽  
pp. 319
Author(s):  
Dana Ment ◽  
Hysen Kokiçi ◽  
Enrico de Lillo
Keyword(s):  
Beauveria Bassiana ◽  
Microbial Control ◽  
Soil Surface ◽  
Field Application ◽  
Post Inoculation ◽  
Metarhizium Brunneum ◽  
Root Localization ◽  
Neonate Larvae ◽  
Preventative Approach ◽  
Colonization Rates

Management of the Mediterranean flat-headed root-borer, Capnodis tenebrionis, is critical due to the larvae’s root localization. Neonate larvae can be exposed to natural enemies before penetrating the roots. Application of Metarhizium brunneum strain Mb7 and Beauveria bassiana strain GHA formulations on rice granules was investigated for their efficacy against C. tenebrionis larvae. Mb7 application, evaluated on apricot twigs, significantly and dose-dependently reduced colonization rates of neonates, with highest mortality at 108 conidia/g soil. Neonate susceptibility to Mb7 and GHA was evaluated on potted rootstocks (GF677 almond × peach, 2729 plum) planted in entomopathogenic fungi (EPF)-premixed soil (1.3–1.6 × 105 conidia/cm3 soil) or in EPF-free soil surface-treated with 5 g Mb7 fungal granules (1.25 × 109 conidia). Larval colonization rates were reduced 7.4-fold in 2729 by both fungi; only Mb7 completely prevented colonization of GF677 by larvae. Larvae inside plant galleries exhibited mycosis with EPF-treated soils and both fungi proliferated on larval frass. Mb7 conidia germinated in the rhizosphere of GF677, and conidia of both fungi remained viable throughout the trial. Galleria baiting technique was used on EPF-treated soil to evaluate EPF infectivity over time; Mb7 and GHA persisted 180 and 90 days post inoculation, respectively. The formulation (fungus-covered rice grains), delivery method (mixing with soil) and persistence (3–6 months) of Mb7 and GHA are feasible for potential field application to control C. tenebrionis.

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