scholarly journals High-pressure 1H NMR study of pressure-induced structural changes in the heme environments of metcyanomyoglobins

2003 ◽  
Vol 12 (2) ◽  
pp. 207-217 ◽  
Author(s):  
R. Kitahara
Keyword(s):  
High Pressure ◽  
1H Nmr ◽  
Structural Changes ◽  
Nmr Study

scholarly journals 15N and 1H NMR study of histidine containing protein (hpr) from staphylococcus carnosus at high pressure

2008 ◽  
Vol 9 (4) ◽  
pp. 693-703 ◽  
Author(s):  
Hans Robert Kalbitzer ◽  
Adrian Görler ◽  
Hua Li ◽  
Peter V. Dubovskii ◽  
Wolfgang Hengstenberg ◽  
...  
Keyword(s):  
High Pressure ◽  
1H Nmr ◽  
Staphylococcus Carnosus ◽  
Nmr Study

High-temperature and high-pressure 1H NMR study on carbonization of pitch

Carbon ◽  
1984 ◽  
Vol 22 (4-5) ◽  
pp. 433-436 ◽  
Author(s):  
S. Shimokawa ◽  
E. Yamada ◽  
T. Yokono ◽  
S. Iyama ◽  
Y. Sanada
Keyword(s):  
High Pressure ◽  
High Temperature ◽  
1H Nmr ◽  
Nmr Study

1H-NMR Study of the Impact of High Pressure and Thermal Processing on Cell Membrane Integrity of Onions

2010 ◽  
Vol 75 (7) ◽  
pp. E417-E425 ◽  
Author(s):  
Maria E. Gonzalez ◽  
Diane M. Barrett ◽  
Michael J. McCarthy ◽  
Frank J. Vergeldt ◽  
Edo Gerkema ◽  
...  
Keyword(s):  
High Pressure ◽  
Cell Membrane ◽  
1H Nmr ◽  
Thermal Processing ◽  
Membrane Integrity ◽  
Cell Membrane Integrity ◽  
Nmr Study ◽  
The Impact

High-temperature high-pressure 1H-NMR study on formation of carbon spherules

Carbon ◽  
1986 ◽  
Vol 24 (6) ◽  
pp. 771-772 ◽  
Author(s):  
S. Shimokawa ◽  
E. Yamada ◽  
T. Yokono ◽  
J. Yamada ◽  
Y. Sanada ◽  
...  
Keyword(s):  
High Pressure ◽  
High Temperature ◽  
1H Nmr ◽  
Nmr Study ◽  
High Temperature High Pressure

1H-NMR study of Mott insulator κ -(ET)2Cu2(CN)3with isotropic triangular lattice

2004 ◽  
Vol 114 ◽  
pp. 377-378 ◽  
Author(s):  
Y. Shimizu ◽  
K. Miyagawa ◽  
K. Oda ◽  
K. Kanoda ◽  
M. Maesato ◽  
...  
Keyword(s):  
1H Nmr ◽  
Triangular Lattice ◽  
Mott Insulator ◽  
Nmr Study

Expression and Solution NMR Study of Multi-site Phosphomimetic Mutant BCL-2 Protein

2019 ◽  
Vol 26 (6) ◽  
pp. 449-457
Author(s):  
Ting Song ◽  
Keke Cao ◽  
Yu dan Fan ◽  
Zhichao Zhang ◽  
Zong W. Guo ◽  
...  
Keyword(s):  
Structural Change ◽  
Structural Biology ◽  
Quantum Coherence ◽  
Structural Changes ◽  
Solution Nmr ◽  
Flexible Loop ◽  
Loop Region ◽  
Loop Domain ◽  
Nmr Study ◽  
Binding Groove

Background: The significance of multi-site phosphorylation of BCL-2 protein in the flexible loop domain remains controversial, in part due to the lack of structural biology studies of phosphorylated BCL-2. Objective: The purpose of the study is to explore the phosphorylation induced structural changes of BCL-2 protein. Methods: We constructed a phosphomietic mutant BCL-2(62-206) (t69e, s70e and s87e) (EEEBCL- 2-EK (62-206)), in which the BH4 domain and the part of loop region was truncated (residues 2-61) to enable a backbone resonance assignment. The phosphorylation-induced structural change was visualized by overlapping a well dispersed 15N-1H heteronuclear single quantum coherence (HSQC) NMR spectroscopy between EEE-BCL-2-EK (62-206) and BCL-2. Results: The EEE-BCL-2-EK (62-206) protein reproduced the biochemical and cellular activity of the native phosphorylated BCL-2 (pBCL-2), which was distinct from non-phosphorylated BCL-2 (npBCL-2) protein. Some residues in BH3 binding groove occurred chemical shift in the EEEBCL- 2-EK (62-206) spectrum, indicating that the phosphorylation in the loop region induces a structural change of active site. Conclusion: The phosphorylation of BCL-2 induced structural change in BH3 binding groove.

A 15N, 13C, and 1H NMR study of reaction products from arylguanidines and chloroformate esters

1991 ◽  
Vol 56 (7) ◽  
pp. 1505-1511 ◽  
Author(s):  
Antonín Lyčka ◽  
Karel Palát
Keyword(s):  
1H Nmr ◽  
Nmr Spectra ◽  
Reaction Products ◽  
H Nmr ◽  
Nmr Study

The 15N, 13C, and 1H NMR spectra of the reaction products from arylguanidines with two mols of chloroformate esters have been measured. With application of the corresponding 15N isotopomer it has been proved that the reaction products have the structures IIIa-IIIc.

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