scholarly journals Binding specificity and the ligand dissociation process in the E. coli biotin holoenzyme synthetase

2009 ◽  
Vol 11 (3) ◽  
pp. 558-570 ◽  
Author(s):  
Keehwan Kwon ◽  
Emily D. Streaker ◽  
Dorothy Beckett
Keyword(s):  
Binding Specificity ◽  
Dissociation Process ◽  
E Coli ◽  
Ligand Dissociation

scholarly journals Echovirus-9 protein 2C binds single-stranded RNA unspecifically

2000 ◽  
Vol 81 (10) ◽  
pp. 2481-2484 ◽  
Author(s):  
Marcus Klein ◽  
Hans J. Eggers ◽  
Birgit Nelsen-Salz
Keyword(s):  
Rna Binding ◽  
Binding Specificity ◽  
Secondary Structures ◽  
Multiple Functions ◽  
E Coli ◽  
Linear Dna ◽  
Gel Retardation ◽  
Rna Fragments ◽  
Poliovirus Protein

Polypeptide 2C is essential for picornavirus replication. Although many data on multiple functions of this highly conserved protein are available, the mechanism of RNA binding is still obscure. In this work, protein 2C of echovirus-9 strain Barty was expressed as a histidine-tagged protein in E. coli followed by nondenaturing purification to homogeneity. After incubation of 2C protein with different kinds of RNA fragments, binding was shown in gel retardation assays. Competition experiments revealed that 2C targets linear RNA unspecifically; however, single-stranded linear DNA does not react with this protein. In contrast to poliovirus, protein 2C of echovirus-9 only recognizes RNA with a low content of secondary structures. This may be a first hint of a different binding specificity of 2C in echo- and polioviruses.

scholarly journals Constructing and purifying megabodies starting from individual nanobody sequences

2021 ◽  
Author(s):  
Tomasz Uchański ◽  
Baptiste Fischer ◽  
Valentina Kalichuk ◽  
Alexandre Wohlkönig ◽  
Thomas Zögg ◽  
...  
Keyword(s):  
Molecular Weight ◽  
Particle Size ◽  
Single Particle ◽  
Binding Specificity ◽  
Preferential Orientation ◽  
Antigen Binding ◽  
Protein Scaffolds ◽  
E Coli ◽  
Air Interfaces ◽  
Chimeric Molecules

Abstract Megabodies are engineered nanobodies that help overcome two major obstacles that limit the resolution of single-particle cryo-EM reconstructions: particle size and preferential orientation at the water-air interfaces. Here we describe how nanobodies can be rigidly grafted onto selected protein scaffolds (HopQ or YgjK) to increase their molecular weight while retaining the full antigen binding specificity and affinity. We also describe the protocols to purify these chimeric molecules from the periplasm of E. coli.

scholarly journals RNA-binding specificity of E. coli NusA

2009 ◽  
Vol 37 (14) ◽  
pp. 4736-4742 ◽  
Author(s):  
S. Prasch ◽  
M. Jurk ◽  
R. S. Washburn ◽  
M. E. Gottesman ◽  
B. M. Wohrl ◽  
...  
Keyword(s):  
Rna Binding ◽  
Binding Specificity ◽  
E Coli

Stereomutation at hexacoordinate silicon by a ligand-dissociation process

1984 ◽  
Vol 106 (14) ◽  
pp. 3992-3994 ◽  
Author(s):  
William B. Farnham ◽  
John F. Whitney
Keyword(s):  
Dissociation Process ◽  
Ligand Dissociation

scholarly journals Binding Specificity of E. coli SSB C-Terminal Tails to SIPs

2019 ◽  
Vol 116 (3) ◽  
pp. 77a
Author(s):  
Min Kyung Shinn ◽  
Alexander G. Kozlov ◽  
Timothy M. Lohman
Keyword(s):  
Binding Specificity ◽  
E Coli
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