scholarly journals Mutations in domaina′ of protein disulfide isomerase affect the folding pathway of bovine pancreatic ribonuclease A

2003 ◽  
Vol 12 (5) ◽  
pp. 939-952 ◽  
Author(s):  
Margherita Ruoppolo ◽  
Stefania Orrù ◽  
Fabio Talamo ◽  
Johanna Ljung ◽  
Annamari Pirneskoski ◽  
...  
Keyword(s):  
Protein Disulfide Isomerase ◽  
Ribonuclease A ◽  
Folding Pathway ◽  
Disulfide Isomerase ◽  
Pancreatic Ribonuclease ◽  
Pancreatic Ribonuclease A ◽  
Protein Disulfide

Functional analysis of tunicamycin-inducible gene A polypeptide from Aspergillus niger

2005 ◽  
Vol 83 (5) ◽  
pp. 654-658 ◽  
Author(s):  
Yurong Liang ◽  
Wei Li ◽  
Qing Ma ◽  
Yuying Zhang
Keyword(s):  
Functional Analysis ◽  
Aspergillus Niger ◽  
Substrate Specificity ◽  
Protein Disulfide Isomerase ◽  
Ribonuclease A ◽  
Chaperone Activity ◽  
Disulfide Isomerase ◽  
Chaperone Protein ◽  
Protein Disulfide ◽  
Inducible Gene

Tunicamycin-inducible gene A polypeptide (TIGA) is a member of the protein disulfide isomerase (PDI) family and is suggested to facilitate the folding of nascent polypeptides. The functional properties of TIGA were investigated here. TIGA acted as an isomerase, catalyzing the refolding of denatured and reduced ribonuclease A. TIGA also exhibited chaperone activity in the refolding of denatured prochymosin but not in the refolding of glyceraldehyde 3-phosphate dehydrogenase (GAPDH), indicating that it had substrate specificity with respect to chaperone activity. Detailed study with a series of thioredoxin-motif (trx-motif) mutants revealed that the 2 trx-motifs of TIGA were not equal in activity. The N-terminal trx-motif was more active than the C-terminal trx-motif, and the first cysteine in each trx-motif was necessary for isomerase activity.Key words: tunicamycin-inducible gene A polypeptide (TIGA), protein disulfide isomerase, chaperone, protein refolding.

Folding pathway of guanidine-denatured disulfide-intact wild-type and mutant bovine pancreatic ribonuclease A

1994 ◽  
Vol 13 (4) ◽  
pp. 409-421 ◽  
Author(s):  
Robert W. Dodge ◽  
John H. Laity ◽  
David M. Rothwarf ◽  
Sakurako Shimotakahara ◽  
Harold A. Scheraga
Keyword(s):  
Ribonuclease A ◽  
Folding Pathway ◽  
Wild Type ◽  
Pancreatic Ribonuclease ◽  
Pancreatic Ribonuclease A
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