scholarly journals Heterogeneity in the structural dynamics of Sulfolobus solfataricus β-glycosidase revealed by electron paramagnetic resonance and frequency domain fluorometry

2009 ◽  
Vol 11 (11) ◽  
pp. 2535-2544 ◽  
Author(s):  
Evgenia Lozinsky ◽  
Ferdinando Febbraio ◽  
Alexander I. Shames ◽  
Gertz I. Likhtenshtein ◽  
Ettore Bismuto ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Frequency Domain ◽  
Structural Dynamics ◽  
Sulfolobus Solfataricus ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic ◽  
Frequency Domain Fluorometry

scholarly journals Structural Dynamics of Calcium Transport Proteins Detected by Saturation Transfer Electron Paramagnetic Resonance

2012 ◽  
Vol 102 (3) ◽  
pp. 709a
Author(s):  
Jesse E. McCaffrey ◽  
Zachary M. James ◽  
Kurt D. Torgersen ◽  
Christine B. Karim ◽  
Edmund C. Howard ◽  
...  
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Structural Dynamics ◽  
Calcium Transport ◽  
Transport Proteins ◽  
Saturation Transfer ◽  
Paramagnetic Resonance ◽  
Electron Paramagnetic

scholarly journals Probing Structural Dynamics of Membrane Proteins Using Electron Paramagnetic Resonance Spectroscopic Techniques

Biophysica ◽  
2021 ◽  
Vol 1 (2) ◽  
pp. 106-125
Author(s):  
Indra D. Sahu ◽  
Gary A. Lorigan
Keyword(s):  
Electron Paramagnetic Resonance ◽  
Membrane Proteins ◽  
Structural Dynamics ◽  
Conformational Dynamics ◽  
Biological Significance ◽  
Spectroscopic Techniques ◽  
Paramagnetic Resonance ◽  
Site Directed Spin Labeling ◽  
Living Organisms ◽  
Electron Paramagnetic

Membrane proteins are essential for the survival of living organisms. They are involved in important biological functions including transportation of ions and molecules across the cell membrane and triggering the signaling pathways. They are targets of more than half of the modern medical drugs. Despite their biological significance, information about the structural dynamics of membrane proteins is lagging when compared to that of globular proteins. The major challenges with these systems are low expression yields and lack of appropriate solubilizing medium required for biophysical techniques. Electron paramagnetic resonance (EPR) spectroscopy coupled with site directed spin labeling (SDSL) is a rapidly growing powerful biophysical technique that can be used to obtain pertinent structural and dynamic information on membrane proteins. In this brief review, we will focus on the overview of the widely used EPR approaches and their emerging applications to answer structural and conformational dynamics related questions on important membrane protein systems.

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