Quantitative phase visualization and aberration correction using digital holographic microscope

2013 ◽  
Author(s):  
P. Vimal Prabhu ◽  
Renu John
Keyword(s):  
Aberration Correction ◽  
Quantitative Phase ◽  
Phase Visualization

scholarly journals Simultaneous measurement of neurite and neural body mass accumulation via quantitative phase imaging

The Analyst ◽  
2021 ◽  
Author(s):  
Soorya Pradeep ◽  
Tasmia Tasnim ◽  
Huanan Zhang ◽  
Thomas A. Zangle
Keyword(s):  
Body Mass ◽  
Biomass Production ◽  
Simultaneous Measurement ◽  
Neural Differentiation ◽  
Phase Imaging ◽  
Quantitative Phase Imaging ◽  
Quantitative Phase ◽  
Mass Accumulation

Quantitative phase imaging (QPI) used to quantify the mass of soma (cell bodies) and neurites as well as the rates of biomass production due to neurite maturation and formation during neural differentiation.

scholarly journals Characterization of Liposomes Using Quantitative Phase Microscopy (QPM)

Pharmaceutics ◽  
2021 ◽  
Vol 13 (5) ◽  
pp. 590
Author(s):  
Jennifer Cauzzo ◽  
Nikhil Jayakumar ◽  
Balpreet Singh Ahluwalia ◽  
Azeem Ahmad ◽  
Nataša Škalko-Basnet
Keyword(s):  
Rapid Development ◽  
Fluorescent Labeling ◽  
Label Free ◽  
Batch Mode ◽  
Full Field ◽  
Quantitative Phase ◽  
Phase Microscopy ◽  
Quantitative Phase Microscopy ◽  
Tracking Ability

The rapid development of nanomedicine and drug delivery systems calls for new and effective characterization techniques that can accurately characterize both the properties and the behavior of nanosystems. Standard methods such as dynamic light scattering (DLS) and fluorescent-based assays present challenges in terms of system’s instability, machine sensitivity, and loss of tracking ability, among others. In this study, we explore some of the downsides of batch-mode analyses and fluorescent labeling, while introducing quantitative phase microscopy (QPM) as a label-free complimentary characterization technique. Liposomes were used as a model nanocarrier for their therapeutic relevance and structural versatility. A successful immobilization of liposomes in a non-dried setup allowed for static imaging conditions in an off-axis phase microscope. Image reconstruction was then performed with a phase-shifting algorithm providing high spatial resolution. Our results show the potential of QPM to localize subdiffraction-limited liposomes, estimate their size, and track their integrity over time. Moreover, QPM full-field-of-view images enable the estimation of a single-particle-based size distribution, providing an alternative to the batch mode approach. QPM thus overcomes some of the drawbacks of the conventional methods, serving as a relevant complimentary technique in the characterization of nanosystems.

Low-order aberration correction of the TMT tertiary mirror prototype based on a warping harness

2018 ◽  
Vol 57 (7) ◽  
pp. 1662 ◽  
Author(s):  
Linchu Han ◽  
Chengzhi Liu ◽  
Cunbo Fan ◽  
Zhenwei Li ◽  
Jingxu Zhang ◽  
...  
Keyword(s):  
Aberration Correction ◽  
Low Order

scholarly journals Challenges of Mechanochemistry: Is In Situ Real-Time Quantitative Phase Analysis Always Reliable? A Case Study of Organic Salt Formation

2017 ◽  
Vol 4 (9) ◽  
pp. 1700132 ◽  
Author(s):  
Adam A. L. Michalchuk ◽  
Ivan A. Tumanov ◽  
Sumit Konar ◽  
Simon A. J. Kimber ◽  
Colin R. Pulham ◽  
...  
Keyword(s):  
Real Time ◽  
Phase Analysis ◽  
Organic Salt ◽  
Quantitative Phase Analysis ◽  
Salt Formation ◽  
Quantitative Phase
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