Photoacoustic imaging of breast microcalcifications: A validation study with 3-dimensional ex vivo data

2012 ◽  
Author(s):  
Jeeun Kang ◽  
Eun-Kyung Kim ◽  
Tai-kyong Song ◽  
Jin Ho Chang
Keyword(s):  
Validation Study ◽  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
3 Dimensional

scholarly journals Fabrication of Coaxial and Confocal Transducer Based on Sol-Gel Composite Material for Optical Resolution Photoacoustic Microscopy

Diagnostics ◽  
2019 ◽  
Vol 10 (1) ◽  
pp. 6
Author(s):  
Masayuki Tanabe ◽  
Tai Chieh Wu ◽  
Makiko Kobayashi ◽  
Che Hua Yang
Keyword(s):  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Sol Gel ◽  
Optical Resolution ◽  
Maximum Amplitude ◽  
Photoacoustic Signal ◽  
Center Frequency ◽  
Photoacoustic Microscopy ◽  
3 Dimensional ◽  
Pulse Echo

We have newly developed coaxial and confocal optical-resolution photoacoustic microscopy based on sol-gel composite materials. This transducer contains a concave-shaped piezoelectric layer with a focus depth of 5 mm and a hole with a diameter of 3 mm at the center to pass a laser beam into a phantom. Therefore, this system can directly detect an excited photoacoustic signal without prisms or acoustic lenses. We demonstrate the capability of the system through pulse-echo and photoacoustic imaging experiments. The center frequency of the fabricated transducer is approximately 7 MHz, and its relative bandwidth is 86%. An ex-vivo experiment is conducted, and photoacoustic signals are clearly obtained. As a result, 2- and 3-dimensional maximum amplitude projection images are reconstructed.

Paradoxical paramagnetic calcifications in the globus pallidus: An ex vivo MR investigation and histological validation study

2021 ◽  
Author(s):  
Jinhee Jang ◽  
Yoonho Nam ◽  
Sung Won Jung ◽  
Tae‐Ryong Riew ◽  
Sang Hyun Kim ◽  
...  
Keyword(s):  
Globus Pallidus ◽  
Validation Study ◽  
Ex Vivo ◽  
Histological Validation

scholarly journals 3-Dimensional ventricular electrical activation pattern assessed from a novel high-frequency electrocardiographic imaging technique: principles and clinical importance

2021 ◽  
Vol 11 (1) ◽  
Author(s):  
Pavel Jurak ◽  
Laura R. Bear ◽  
Uyên Châu Nguyên ◽  
Ivo Viscor ◽  
Petr Andrla ◽  
...  
Keyword(s):  
High Frequency ◽  
Ex Vivo ◽  
Clinical Importance ◽  
Electrical Activation ◽  
Bundle Branch Block ◽  
Electrocardiographic Imaging ◽  
3 Dimensional ◽  
Intraventricular Conduction ◽  
Clinical Measurements ◽  
Activation Times

AbstractThe study introduces and validates a novel high-frequency (100–400 Hz bandwidth, 2 kHz sampling frequency) electrocardiographic imaging (HFECGI) technique that measures intramural ventricular electrical activation. Ex-vivo experiments and clinical measurements were employed. Ex-vivo, two pig hearts were suspended in a human-torso shaped tank using surface tank electrodes, epicardial electrode sock, and plunge electrodes. We compared conventional epicardial electrocardiographic imaging (ECGI) with intramural activation by HFECGI and verified with sock and plunge electrodes. Clinical importance of HFECGI measurements was performed on 14 patients with variable conduction abnormalities. From 3 × 4 needle and 108 sock electrodes, 256 torso or 184 body surface electrodes records, transmural activation times, sock epicardial activation times, ECGI-derived activation times, and high-frequency activation times were computed. The ex-vivo transmural measurements showed that HFECGI measures intramural electrical activation, and ECGI-HFECGI activation times differences indicate endo-to-epi or epi-to-endo conduction direction. HFECGI-derived volumetric dyssynchrony was significantly lower than epicardial ECGI dyssynchrony. HFECGI dyssynchrony was able to distinguish between intraventricular conduction disturbance and bundle branch block patients.

scholarly journals Towards Transabdominal Functional Photoacoustic Imaging of the Placenta: Improvement in Imaging Depth Through Optimization of Light Delivery

2021 ◽  
Author(s):  
Kristie Huda ◽  
Kenneth F. Swan ◽  
Cecilia T. Gambala ◽  
Gabriella C. Pridjian ◽  
Carolyn L. Bayer
Keyword(s):  
Delivery System ◽  
Light Propagation ◽  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Incidence Angle ◽  
Incident Angle ◽  
Placental Tissue ◽  
Light Delivery ◽  
Imaging Depth ◽  
The Impact

AbstractFunctional photoacoustic imaging of the placenta could provide an innovative tool to diagnose preeclampsia, monitor fetal growth restriction, and determine the developmental impacts of gestational diabetes. However, transabdominal photoacoustic imaging is limited in imaging depth due to the tissue’s scattering and absorption of light. The aim of this paper was to investigate the impact of geometry and wavelength on transabdominal light delivery. Our methods included the development of a multilayer model of the abdominal tissue and simulation of the light propagation using Monte Carlo methods. A bifurcated light source with varying incident angle of light, distance between light beams, and beam area was simulated to analyze the effect of light delivery geometry on the fluence distribution at depth. The impact of wavelength and the effects of variable thicknesses of adipose tissue and muscle were also studied. Our results showed that the beam area plays a major role in improving the delivery of light to deep tissue, in comparison to light incidence angle or distance between the bifurcated fibers. Longer wavelengths, with incident fluence at the maximum permissible exposure limit, also increases fluence within deeper tissue. We validated our simulations using a commercially available light delivery system and ex vivo human placental tissue. Additionally, we compared our optimized light delivery to a commercially available light delivery system, and conclude that our optimized geometry could improve imaging depth more than 1.6×, bringing the imaging depth to within the needed range for transabdominal imaging of the human placenta.

scholarly journals Preclinical Ultrasound-Guided Photoacoustic Imaging of the Placenta in Normal and Pathologic Pregnancy

2018 ◽  
Vol 17 ◽  
pp. 153601211880272 ◽  
Author(s):  
Liliya M. Yamaleyeva ◽  
K. Bridget Brosnihan ◽  
Lane M. Smith ◽  
Yao Sun
Keyword(s):  
Oxygen Saturation ◽  
Early Identification ◽  
Photoacoustic Imaging ◽  
Three Dimensional ◽  
Normal Pregnancy ◽  
Preclinical Study ◽  
Ultrasound Guided ◽  
3 Dimensional ◽  
Sensitive Tool ◽  
Different Levels

Placental oxygenation varies throughout pregnancy. The detection of early changes in placental oxygenation as pregnancy progresses is important for early identification of preeclampsia or other complications. This invited commentary discusses a recent preclinical study on the application of 3-dimensional photoacoustic imaging (PAI) for assessment of regional variations in placental oxygenation and longitudinal analysis of differences in placental oxygenation throughout normal pregnancy and pregnancy associated with hypertension or placental insufficiency in mice. Three-dimensional PAI more accurately reflects oxygen saturation, hemoglobin concentrations, and changes in oxygen saturation in whole placenta compared to 2-dimensional imaging. These studies suggest that PAI is a sensitive tool to detect different levels of oxygen saturation in the placental and fetal vasculature in pathologic and normal pregnancy in mice.

Photoacoustic imaging of breast microcalcifications: A validation study with 3-dimensionalex vivodata and spectrophotometric measurement

2013 ◽  
Vol 8 (1-2) ◽  
pp. 71-80 ◽  
Author(s):  
Jeeun Kang ◽  
Eun-kyung Kim ◽  
Ga Ram Kim ◽  
Changhan Yoon ◽  
Tai-kyong Song ◽  
...  
Keyword(s):  
Validation Study ◽  
Photoacoustic Imaging ◽  
Spectrophotometric Measurement

scholarly journals Efficient Photoacoustic Imaging With Biomimetic Mesoporous Silica-Based Nanoparticles

2021 ◽  
Vol 9 ◽  
Author(s):  
Chuangjia Huang ◽  
Xiaoling Guan ◽  
Hui Lin ◽  
Lu Liang ◽  
Yingling Miao ◽  
...  
Keyword(s):  
Mesoporous Silica ◽  
Near Infrared ◽  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Mesoporous Silica Nanoparticles ◽  
Good Biocompatibility ◽  
Targeting Ability ◽  
Body Clearance

Indocyanine green (ICG), a near-infrared (NIR) fluorescent dye approved by the Food and Drug Administration (FDA), has been extensively used as a photoacoustic (PA) probe for PA imaging. However, its practical application is limited by poor photostability in water, rapid body clearance, and non-specificity. Herein, we fabricated a novel biomimetic nanoprobe by coating ICG-loaded mesoporous silica nanoparticles with the cancer cell membrane (namely, CMI) for PA imaging. This probe exhibited good dispersion, large loading efficiency, good biocompatibility, and homologous targeting ability to Hela cells in vitro. Furthermore, the in vivo and ex vivo PA imaging on Hela tumor-bearing nude mice demonstrated that CMI could accumulate in tumor tissue and display a superior PA imaging efficacy compared with free ICG. All these results demonstrated that CMI might be a promising contrast agent for PA imaging of cervical carcinoma.

scholarly journals Towards Clinical Translation of LED-Based Photoacoustic Imaging: A Review

Sensors ◽  
2020 ◽  
Vol 20 (9) ◽  
pp. 2484 ◽  
Author(s):  
Yunhao Zhu ◽  
Ting Feng ◽  
Qian Cheng ◽  
Xueding Wang ◽  
Sidan Du ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Photoacoustic Imaging ◽  
Imaging Techniques ◽  
Clinical Translation ◽  
Key Factors ◽  
Pilot Studies ◽  
History Of ◽  
Molecular Components ◽  
Clinical Potential

Photoacoustic imaging, with the capability to provide simultaneous structural, functional, and molecular information, is one of the fastest growing biomedical imaging modalities of recent times. As a hybrid modality, it not only provides greater penetration depth than the purely optical imaging techniques, but also provides optical contrast of molecular components in the living tissue. Conventionally, photoacoustic imaging systems utilize bulky and expensive class IV lasers, which is one of the key factors hindering the clinical translation of this promising modality. Use of LEDs which are portable and affordable offers a unique opportunity to accelerate the clinical translation of photoacoustics. In this paper, we first review the development history of LED as an illumination source in biomedical photoacoustic imaging. Key developments in this area, from point-source measurements to development of high-power LED arrays, are briefly discussed. Finally, we thoroughly review multiple phantom, ex-vivo, animal in-vivo, human in-vivo, and clinical pilot studies and demonstrate the unprecedented preclinical and clinical potential of LED-based photoacoustic imaging.

Sign in / Sign up

Export Citation Format

Share Document