Porous Silicon Based Protocol for the Rapid and Real-Time Monitoring of Biorecognition Between Human IgG and Protein A Using Functionalized Superparamagnetic Beads

2012 ◽  
Vol 48 (11) ◽  
pp. 2846-2849 ◽  
Author(s):  
Pil Ju Ko ◽  
Ryousuke Ishikawa ◽  
Tsukasa Takamura ◽  
Honglae Sohn ◽  
Adarsh Sandhu
Keyword(s):  
Porous Silicon ◽  
Real Time ◽  
Protein A ◽  
Real Time Monitoring ◽  
Human Igg ◽  
Superparamagnetic Beads ◽  
Silicon Based

scholarly journals Porous Silicon-Based Biosensors: Towards Real-Time Optical Detection of Target Bacteria in the Food Industry

2016 ◽  
Vol 6 (1) ◽  
Author(s):  
Naama Massad-Ivanir ◽  
Giorgi Shtenberg ◽  
Nitzan Raz ◽  
Christel Gazenbeek ◽  
Dries Budding ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Real Time ◽  
Food Industry ◽  
Optical Detection ◽  
Silicon Based

Cathepsin D: Removal strategy on protein A chromatography, near real time monitoring and characterisation during monoclonal antibody production

2019 ◽  
Vol 305 ◽  
pp. 51-60
Author(s):  
Tingting Cui ◽  
Bertie Chi ◽  
Jenny Heidbrink Thompson ◽  
Toyin Kasali ◽  
Christopher Sellick ◽  
...  
Keyword(s):  
Monoclonal Antibody ◽  
Real Time ◽  
Antibody Production ◽  
Cathepsin D ◽  
Protein A ◽  
Real Time Monitoring ◽  
Monoclonal Antibody Production

scholarly journals Real-time monitoring of sustained drug release using the optical properties of porous silicon photonic crystal particles

Biomaterials ◽  
2011 ◽  
Vol 32 (7) ◽  
pp. 1957-1966 ◽  
Author(s):  
Elizabeth C. Wu ◽  
Jennifer S. Andrew ◽  
Lingyun Cheng ◽  
William R. Freeman ◽  
Lindsey Pearson ◽  
...  
Keyword(s):  
Optical Properties ◽  
Photonic Crystal ◽  
Porous Silicon ◽  
Drug Release ◽  
Real Time ◽  
Real Time Monitoring ◽  
Sustained Drug Release ◽  
Silicon Photonic

Fabrication and characterization of a chemically oxidized-nanostructured porous silicon based biosensor implementing orienting protein A

2014 ◽  
Vol 115 ◽  
pp. 310-316 ◽  
Author(s):  
Nelson Naveas ◽  
Jacobo Hernandez-Montelongo ◽  
Ruth Pulido ◽  
Vicente Torres-Costa ◽  
Raúl Villanueva-Guerrero ◽  
...  
Keyword(s):  
Porous Silicon ◽  
Protein A ◽  
Fabrication And Characterization ◽  
Silicon Based

Development of a Porous Silicon Based Biosensor

1998 ◽  
Vol 536 ◽  
Author(s):  
Keiki-Pua S. Dancil ◽  
Douglas P. Greiner ◽  
Michael J. Sailor
Keyword(s):  
Porous Silicon ◽  
Protein Interactions ◽  
Optical Cavity ◽  
Protein A ◽  
Longitudinal Optical ◽  
Thin Layers ◽  
Protein Protein Interactions ◽  
Cavity Modes ◽  
Fabry Perot ◽  
Silicon Based

AbstractIn this paper we demonstrate that porous silicon (PS) can be used as an immobilization matrix and a transducer for biosensor applications. Thin layers of PS were fabricated showing fine structure in their reflection spectra, characteristic of longitudinal optical cavity modes, or Fabry-Perot interference fringes. The PS surface was modified by covalently bonding streptavidin to a heterobifunctional linker immobilized to the surface using common silane chemistry. The mode spacing and wavelength in the interference spectrum was modified, by displacing buffer and introducing proteins into the PS layer. Protein-protein interactions between immobilized Streptavidin and biotinylated Protein A followed by Protein A and IgG were detected. The surface was regenerated during the course of the experiment showing reversibility of the sensor at the third layer.

scholarly journals Real-time monitoring and control of the load phase of a protein A capture step

2016 ◽  
Vol 114 (2) ◽  
pp. 368-373 ◽  
Author(s):  
Matthias Rüdt ◽  
Nina Brestrich ◽  
Laura Rolinger ◽  
Jürgen Hubbuch
Keyword(s):  
Real Time ◽  
Protein A ◽  
Monitoring And Control ◽  
Real Time Monitoring ◽  
And Control

scholarly journals Real Time Monitoring of a UV Light-Assisted Biofunctionalization Protocol Using a Nanophotonic Biosensor

Biosensors ◽  
2018 ◽  
Vol 9 (1) ◽  
pp. 6 ◽  
Author(s):  
Jad Sabek ◽  
Luis Torrijos-Morán ◽  
Amadeu Griol ◽  
Zeneida Díaz Betancor ◽  
María-José Bañuls Polo ◽  
...  
Keyword(s):  
Real Time ◽  
Uv Light ◽  
Silicon On Insulator ◽  
Hinge Region ◽  
Real Time Monitoring ◽  
Silicon Based

A protocol for the covalent biofunctionalization of silicon-based biosensors using a UV light-induced thiol–ene coupling (TEC) reaction has been developed. This biofunctionalization approach has been used to immobilize half antibodies (hIgG), which have been obtained by means of a tris(2-carboxyethyl)phosphine (TCEP) reduction at the hinge region, to the surface of a vinyl-activated silicon-on-insulator (SOI) nanophotonic sensing chip. The response of the sensing structures within the nanophotonic chip was monitored in real time during the biofunctionalization process, which has allowed us to confirm that the bioconjugation of the thiol-terminated bioreceptors onto the vinyl-activated sensing surface is only initiated upon UV light photocatalysis.

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