Three-Dimensional Multilayered Optical Memory Using Two-Photon Induced Reduction of Au$^{3+}$ Doped in PMMA

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James W. M. Chon ◽  
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Min Gu

1998 ◽  
Vol 28 (6) ◽  
pp. 547-554 ◽  
Author(s):  
Denis A Akimov ◽  
Aleksei M Zheltikov ◽  
Nikolai I Koroteev ◽  
Sergey A Magnitskiy ◽  
A N Naumov ◽  
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2000 ◽  
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S. Juodkazis ◽  
M. Watanabe ◽  
H.-B. Sun ◽  
S. Matsuo ◽  
...  

2020 ◽  
Vol 11 (1) ◽  
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Jiang Lan Fan ◽  
Jose A. Rivera ◽  
Wei Sun ◽  
John Peterson ◽  
Henry Haeberle ◽  
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AbstractUnderstanding the structure and function of vasculature in the brain requires us to monitor distributed hemodynamics at high spatial and temporal resolution in three-dimensional (3D) volumes in vivo. Currently, a volumetric vasculature imaging method with sub-capillary spatial resolution and blood flow-resolving speed is lacking. Here, using two-photon laser scanning microscopy (TPLSM) with an axially extended Bessel focus, we capture volumetric hemodynamics in the awake mouse brain at a spatiotemporal resolution sufficient for measuring capillary size and blood flow. With Bessel TPLSM, the fluorescence signal of a vessel becomes proportional to its size, which enables convenient intensity-based analysis of vessel dilation and constriction dynamics in large volumes. We observe entrainment of vasodilation and vasoconstriction with pupil diameter and measure 3D blood flow at 99 volumes/second. Demonstrating high-throughput monitoring of hemodynamics in the awake brain, we expect Bessel TPLSM to make broad impacts on neurovasculature research.


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