scholarly journals Individual Testing Is Optimal for Nonadaptive Group Testing in the Linear Regime

2019 ◽  
Vol 65 (4) ◽  
pp. 2058-2061 ◽  
Author(s):  
Matthew Aldridge
Keyword(s):  
Group Testing ◽  
Linear Regime ◽  
Nonadaptive Group Testing

Nonadaptive Group testing based fiber sensor deployment for multiperson tracking

2006 ◽  
Vol 6 (2) ◽  
pp. 490-494 ◽  
Author(s):  
Yunhui Zheng ◽  
N.P. Pitsianis ◽  
D.J. Brady
Keyword(s):  
Group Testing ◽  
Fiber Sensor ◽  
Sensor Deployment ◽  
Nonadaptive Group Testing

Erasure combinatorial batch codes based on nonadaptive group testing

2018 ◽  
Vol 87 (7) ◽  
pp. 1647-1656 ◽  
Author(s):  
Dongdong Jia ◽  
Sumei Zhang ◽  
Gengsheng Zhang
Keyword(s):  
Group Testing ◽  
Nonadaptive Group Testing

scholarly journals Group Testing Approach for Trinucleotide Repeat Expansion Disorder Screening

2016 ◽  
Vol 62 (10) ◽  
pp. 1401-1408 ◽  
Author(s):  
Kristjan Eerik Kaseniit ◽  
Mark R Theilmann ◽  
Alexander Robertson ◽  
Eric A Evans ◽  
Imran S Haque
Keyword(s):  
Trinucleotide Repeat ◽  
Fragile X ◽  
Group Testing ◽  
Low Complexity ◽  
Repeat Expansion ◽  
Trinucleotide Repeat Expansion ◽  
Cgg Repeats ◽  
Fold Reduction ◽  
Repeat Expansions ◽  
Nonadaptive Group Testing

Abstract BACKGROUND Fragile X syndrome (FXS, OMIM #300624) is an X-linked condition caused by trinucleotide repeat expansions in the 5′ UTR (untranslated region) of the fragile X mental retardation 1 (FMR1) gene. FXS testing is commonly performed in expanded carrier screening and has been proposed for inclusion in newborn screening. However, because pathogenic alleles are long and have low complexity (>200 CGG repeats), FXS is currently tested by a single-plex electrophoresis-resolved PCR assay rather than multiplexed approaches like next-generation sequencing or mass spectrometry. In this work, we sought an experimental design based on nonadaptive group testing that could accurately and reliably identify the size of abnormally expanded FMR1 alleles of males and females. METHODS We developed a new group testing scheme named StairCase (SC) that was designed to the constraints of the FXS testing problem, and compared its performance to existing group testing schemes by simulation. We experimentally evaluated SC's performance on 210 samples from the Coriell Institute biorepositories using pooled PCR followed by capillary electrophoresis on 3 replicates of each of 3 pooling layouts differing by the mapping of samples to pools. RESULTS The SC pooled PCR approach demonstrated perfect classification of samples by clinical category (normal, intermediate, premutation, or full mutation) for 90 positives and 1800 negatives, with a batch of 210 samples requiring only 21 assays. CONCLUSIONS Group testing based on SC is an implementable approach to trinucleotide repeat expansion disorder testing that offers ≥10-fold reduction in assay costs over current single-plex methods.

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