scholarly journals A Practical One-Shot Multispectral Imaging System Using a Single Image Sensor

2015 ◽  
Vol 24 (10) ◽  
pp. 3048-3059 ◽  
Author(s):  
Yusuke Monno ◽  
Sunao Kikuchi ◽  
Masayuki Tanaka ◽  
Masatoshi Okutomi
Keyword(s):  
Multispectral Imaging ◽  
Imaging System ◽  
Image Sensor ◽  
Single Image

scholarly journals SoilCam: A Fully Automated Minirhizotron using Multispectral Imaging for Root Activity Monitoring

Sensors ◽  
2020 ◽  
Vol 20 (3) ◽  
pp. 787
Author(s):  
Gazi Rahman ◽  
Hanif Sohag ◽  
Rakibul Chowdhury ◽  
Khan A. Wahid ◽  
Anh Dinh ◽  
...  
Keyword(s):  
White Light ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Image Sensor ◽  
Motion Blur ◽  
Image Resolution ◽  
In Situ Monitoring ◽  
Geometric Distortion ◽  
Entire Area

A minirhizotron is an in situ root imaging system that captures components of root system architecture dynamics over time. Commercial minirhizotrons are expensive, limited to white-light imaging, and often need human intervention. The implementation of a minirhizotron needs to be low cost, automated, and customizable to be effective and widely adopted. We present a newly designed root imaging system called SoilCam that addresses the above mentioned limitations. The imaging system is multi-modal, i.e., it supports both conventional white-light and multispectral imaging, with fully automated operations for long-term in-situ monitoring using wireless control and access. The system is capable of taking 360° images covering the entire area surrounding the tube. The image sensor can be customized depending on the spectral imaging requirements. The maximum achievable image quality of the system is 8 MP (Mega Pixel)/picture, which is equivalent to a 2500 DPI (dots per inch) image resolution. The length of time in the field can be extended with a rechargeable battery and solar panel connectivity. Offline image-processing software, with several image enhancement algorithms to eliminate motion blur and geometric distortion and to reconstruct the 360° panoramic view, is also presented. The system is tested in the field by imaging canola roots to show the performance advantages over commercial systems.

Portable multispectral imaging system based on Raspberry Pi

Sensor Review ◽  
2017 ◽  
Vol 37 (3) ◽  
pp. 322-329 ◽  
Author(s):  
Nuria Lopez-Ruiz ◽  
Fernando Granados-Ortega ◽  
Miguel Angel Carvajal ◽  
Antonio Martinez-Olmos
Keyword(s):  
Near Infrared ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Light Emitting Diode ◽  
Low Cost ◽  
Image Sensor ◽  
Raspberry Pi ◽  
Image Processing Algorithm ◽  
Content Type

Purpose In this work, the authors aim to present a compact low-cost and portable spectral imaging system for general purposes. The developed system provides information that can be used for a fast in situ identification and classification of samples based on the analysis of captured images. The connectivity of the instrument allows a deeper analysis of the images in an external computer. Design/methodology/approach The wavelength selection of the system is carried out by light multiplexing through a light-emitting diode panel where eight wavelengths covering the spectrum from ultraviolet (UV) to near-infrared region (NIR) have been included. The image sensor used is a red green blue – infrared (RGB-IR) micro-camera controlled by a Raspberry Pi board where a basic image processing algorithm has been programmed. It allows the visualization in an integrated display of the reflectance and the histogram of the images at each wavelength, including UV and NIRs. Findings The prototype has been tested by analyzing several samples in a variety of applications such as detection of damaged, over-ripe and sprayed fruit, classification of different type of plastic materials and determination of properties of water. Originality/value The designed system presents some advantages as being non-expensive and portable in comparison to other multispectral imaging systems. The low-cost and size of the camera module connected to the Raspberry Pi provides a compact instrument for general purposes.

747 Evaluation of immune microenvironment of primary lung cancer and synchronous liver metastasis with multispectral imaging system

2020 ◽  
Vol 8 (Suppl 3) ◽  
pp. A795-A795
Author(s):  
Hyeonbin Cho ◽  
Jae-Hwan Kim ◽  
Ji-Hyun Kim
Keyword(s):  
T Cells ◽  
Liver Metastasis ◽  
Multispectral Imaging ◽  
Immune Cell ◽  
Imaging System ◽  
Cytotoxic T Cells ◽  
Primary Lung Cancer ◽  
Helper T Cells ◽  
Synchronous Liver Metastasis ◽  
Immune Microenvironment

BackgroundCancer immunotherapy (CIT) has substantially improved the survival of cancer patients. However, according to recent studies, liver metastasis was reported to predict worse outcomes for CIT. The main objective of the study is to evaluate the differences in the immune microenvironment (IME) between the primary lung cancer (PL) and synchronous liver metastasis (LM) using a multispectral imaging system.MethodsSix immune markers (CD4, CD8, CTLA-4, granzyme B (GZB), Foxp3 and PD-L1) were analyzed using a multiplex IHC system and inForm program (Akoya) on paired lung-liver samples of 10 patients. Cells were categorized into tumor nest and stroma, and cell counts per unit area were measured for comparison.ResultsThe number of tumor-infiltrating cytotoxic T cells (TIL) in PL (262.5 cells/mm2) was higher than that of LM (113.3 cells/mm2). Additionally, the ratio between the number of TIL and non-TIL was greater in PL (0.31) compared to that of LM (0.26). A similar trend appeared for Helper T cells and regulatory T cells (Treg), as PL consisted of higher numbers of T cells (791.8 Helper T cells/mm2, 195.7 Treg/mm2) than LM (626.3 Helper T cells/mm2, 121.3 Treg/mm2). However, cytotoxic T cells exhibiting GZB+ and CTLA-4- were fewer in PL (140.2 cells/mm2) than in LM (203.3 cells/mm2), and the ratio is 0.69. The mean number of GZB+ TIL in PL (32.5 cells/mm2) was lower than in LM (35.3 cells/mm2), and their proportions among total TIL counts were 0.12 and 0.31, respectively. In PL, GZB+: GZB- ratio is 0.16 while the ratio is 1.91 for LM. A fewer number of TILs exhibiting GZB suggests that PL has lower efficiency in immune response than LM. Another crucial checkpoint receptor that inhibits immune response, CTLA-4, was more prevalent in PL, with CTLA-4+: CTLA-4- ratio in Treg being 0.36 in PL, compared to 0.11 in LM. The tumor proportion score (TPS) of PD-L1 was higher in PL than LM (40.0 vs. 6.6).ConclusionsIn our study, we showed the differences in the numbers of TIL or regulatory T cells and expressions of immune checkpoint receptors (PD-L1, CTLA-4), which significantly influence outcomes for CIT. The study is ongoing to confirm different IME between the PL and LM groups in a larger tumor cohort.ReferencesPeng, Jianhong, et al., Immune Cell Infiltration in the Microenvironment of Liver Oligometastasis from Colorectal Cancer: Intratumoural CD8/CD3 Ratio Is a Valuable Prognostic Index for Patients Undergoing Liver Metastasectomy. Cancers 2019 Dec; 11(12): 1922. https://doi.org/10.3390/cancers11121922Tumeh, Paul C., et al., Liver Metastasis and treatment outcome with Anti-PD-1 monoclonal antibody in patients with melanoma and NSCLC. Cancer Immunol Res 2017 May; 5(5): 417–424. doi: 10.1158/2326-6066.CIR-16-0325Parra, E.R., Immune Cell Profiling in Cancer Using Multiplex Immunofluorescence and Digital Analysis Approaches; Streckfus, C.F., Ed.; IntechOpen: London, UK, 2018; pp. 1–13. doi: 10.5772/intechopen.80380Ribas, A., Hu-Lieskovan, S., What does PD-L1 positive or negative mean?. The Journal of Experimental Medicine 2016;213(13):2835–2840. https://doi.org/10.1084/jem.20161462

scholarly journals Optimized Method for Mapping Inorganic Pigments by Means of Multispectral Imaging Combined with Hyperspectral Spectroscopy for the Study of Vincenzo Pasqualoni’s Wall Painting at the Basilica of S. Nicola in Carcere in Rome

Minerals ◽  
2021 ◽  
Vol 11 (8) ◽  
pp. 839
Author(s):  
Lucilla Pronti ◽  
Giuseppe Capobianco ◽  
Margherita Vendittelli ◽  
Anna Candida Felici ◽  
Silvia Serranti ◽  
...  
Keyword(s):  
Multispectral Imaging ◽  
Imaging System ◽  
Principal Component ◽  
Reflectance Spectroscopy ◽  
Wall Painting ◽  
High Spectral Resolution ◽  
Spectral Difference ◽  
Color Palette ◽  
A Minor ◽  
Nir Reflectance Spectroscopy

Multispectral imaging is a preliminary screening technique for the study of paintings. Although it permits the identification of several mineral pigments by their spectral behavior, it is considered less performing concerning hyperspectral imaging, since a limited number of wavelengths are selected. In this work, we propose an optimized method to map the distribution of the mineral pigments used by Vincenzo Pasqualoni for his wall painting placed at the Basilica of S. Nicola in Carcere in Rome, combining UV/VIS/NIR reflectance spectroscopy and multispectral imaging. The first method (UV/VIS/NIR reflectance spectroscopy) allowed us to characterize pigment layers with a high spectral resolution; the second method (UV/VIS/NIR multispectral imaging) permitted the evaluation of the pigment distribution by utilizing a restricted number of wavelengths. Combining the results obtained from both devices was possible to obtain a distribution map of a pictorial layer with a high accuracy level of pigment recognition. The method involved the joint use of point-by-point hyperspectral spectroscopy and Principal Component Analysis (PCA) to identify the pigments in the color palette and evaluate the possibility to discriminate all the pigments recognized, using a minor number of wavelengths acquired through the multispectral imaging system. Finally, the distribution and the spectral difference of the different pigments recognized in the multispectral images, (in this case: red ochre, yellow ochre, orpiment, cobalt blue-based pigments, ultramarine and chrome green) were shown through PCA false-color images.

scholarly journals Characterizing the Tumor Immune Microenvironment with Tyramide-Based Multiplex Immunofluorescence

2020 ◽  
Vol 25 (4) ◽  
pp. 417-432
Author(s):  
Hidetoshi Mori ◽  
Jennifer Bolen ◽  
Louis Schuetter ◽  
Pierre Massion ◽  
Clifford C. Hoyt ◽  
...  
Keyword(s):  
Tumor Microenvironment ◽  
Multispectral Imaging ◽  
Imaging System ◽  
Predictive Biomarkers ◽  
Cell Types ◽  
Immune Microenvironment ◽  
Tumor Immune Microenvironment ◽  
Manual Processing ◽  
Cell Densities ◽  
Immune Profiling

AbstractMultiplex immunofluorescence (mIF) allows simultaneous antibody-based detection of multiple markers with a nuclear counterstain on a single tissue section. Recent studies have demonstrated that mIF is becoming an important tool for immune profiling the tumor microenvironment, further advancing our understanding of the interplay between cancer and the immune system, and identifying predictive biomarkers of response to immunotherapy. Expediting mIF discoveries is leading to improved diagnostic panels, whereas it is important that mIF protocols be standardized to facilitate their transition into clinical use. Manual processing of sections for mIF is time consuming and a potential source of variability across numerous samples. To increase reproducibility and throughput we demonstrate the use of an automated slide stainer for mIF incorporating tyramide signal amplification (TSA). We describe two panels aimed at characterizing the tumor immune microenvironment. Panel 1 included CD3, CD20, CD117, FOXP3, Ki67, pancytokeratins (CK), and DAPI, and Panel 2 included CD3, CD8, CD68, PD-1, PD-L1, CK, and DAPI. Primary antibodies were first tested by standard immunohistochemistry and single-plex IF, then multiplex panels were developed and images were obtained using a Vectra 3.0 multispectral imaging system. Various methods for image analysis (identifying cell types, determining cell densities, characterizing cell-cell associations) are outlined. These mIF protocols will be invaluable tools for immune profiling the tumor microenvironment.

Development of a Controller Unit for Multispectral Imaging System

2020 ◽  
Author(s):  
Mate Koba ◽  
Attila Trohak ◽  
Norbert Zajzon ◽  
Richard Zoltan Papp ◽  
Marton L. Kiss
Keyword(s):  
Multispectral Imaging ◽  
Imaging System

Acousto-optic tunable filter multispectral imaging system

1992 ◽  
Author(s):  
LI-JEN CHENG ◽  
TIEN-HSIN CHAO ◽  
GEORGE REYES
Keyword(s):  
Multispectral Imaging ◽  
Imaging System ◽  
Tunable Filter
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