Design, Calibration, and Testing of an Automated Near-Infrared Liquid-Crystal Polarimetric Imaging System for Discrimination of Lung Cancer Cells

2015 ◽  
Vol 64 (9) ◽  
pp. 2453-2467 ◽  
Author(s):  
Suman Shrestha ◽  
Jeff Petermann ◽  
Tannaz Farrahi ◽  
Aditi Deshpande ◽  
George C. Giakos
Keyword(s):  
Lung Cancer ◽  
Liquid Crystal ◽  
Cancer Cells ◽  
Near Infrared ◽  
Imaging System ◽  
Lung Cancer Cells ◽  
Polarimetric Imaging

scholarly journals Photochemical generation of the 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) radical from caged nitroxides by near-infrared two-photon irradiation and its cytocidal effect on lung cancer cells

2019 ◽  
Vol 15 ◽  
pp. 863-873 ◽  
Author(s):  
Ayato Yamada ◽  
Manabu Abe ◽  
Yoshinobu Nishimura ◽  
Shoji Ishizaka ◽  
Masashi Namba ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Anticancer Agents ◽  
Near Infrared ◽  
Lung Cancer Cells ◽  
Quantum Yields ◽  
Triggered Release ◽  
Two Photon ◽  
Cytocidal Effect ◽  
Tempo Radical

Novel caged nitroxides (nitroxide donors) with near-infrared two-photon (TP) responsive character, 2,2,6,6-tetramethyl-1-(1-(2-(4-nitrophenyl)benzofuran-6-yl)ethoxy)piperidine (2a) and its regioisomer 2b, were designed and synthesized. The one-photon (OP) (365 ± 10 nm) and TP (710–760 nm) triggered release (i.e., uncaging) of the 2,2,6,6-tetramethylpiperidine-1-oxyl (TEMPO) radical under air atmosphere were discovered. The quantum yields for the release of the TEMPO radical were 2.5% (2a) and 0.8% (2b) in benzene at ≈1% conversion of 2, and 13.1% (2a) and 12.8% (2b) in DMSO at ≈1% conversion of 2. The TP uncaging efficiencies were determined to be 1.1 GM at 740 nm for 2a and 0.22 GM at 730 nm for 2b in benzene. The cytocidal effect of compound 2a on lung cancer cells under photolysis conditions was also assessed to test the efficacy as anticancer agents. In a medium containing 100 μg mL−1 of 2a exposed to light, the number of living cells decreased significantly compared to the unexposed counterparts (65.8% vs 85.5%).

Preferential accumulation of the near infrared heptamethine dye IR-780 in the mitochondria of drug-resistant lung cancer cells

Biomaterials ◽  
2014 ◽  
Vol 35 (13) ◽  
pp. 4116-4124 ◽  
Author(s):  
Yang Wang ◽  
Tao Liu ◽  
Erlong Zhang ◽  
Shenglin Luo ◽  
Xu Tan ◽  
...  
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Near Infrared ◽  
Lung Cancer Cells ◽  
Drug Resistant ◽  
Preferential Accumulation

Inhibitory effects of Actinidia Chinensis planch root extracts (acRoots) on human lung cancer cells through retinoic acid receptor beta

2017 ◽  
Vol 5 (1) ◽  
Author(s):  
Lingyan Wang ◽  
Jiayun Hou ◽  
Minghuan Zheng ◽  
Lin Shi
Keyword(s):  
Lung Cancer ◽  
Cancer Cells ◽  
Human Lung ◽  
Retinoic Acid Receptor ◽  
Lung Cancer Cells ◽  
Human Lung Cancer ◽  
Inhibitory Effects ◽  
The Core ◽  
Human Lung Cancer Cells ◽  
Receptor Beta

Actinidia Chinensis Planch roots (acRoots) are used to treat many cancers, although the anti-tumor mechanism by which acRoots inhibit cancer cell growth remains unclear. The present study aims at investigating inhibitory effects of acRoots on human lung cancer cells and potential mechanisms. Our data demonstrate that the inhibitory effects of acRoots on lung cancer cells depend on genetic backgrounds and phenotypes of cells. We furthermore found the expression of metabolism-associated gene profiles varied between acRoots-hypersensitive (H460) or hyposensitive lung cancer cells (H1299) after screening lung cancer cells with different genetic backgrounds. We selected retinoic acid receptor beta (RARB) as the core target within metabolism-associated core gene networks and evaluated RARB changes and roles in cells treated with acRoots at different concentrations and timeframes. Hypersensitive cancer cells with the deletion of RARB expression did not response to the treatment with acRoots, while RARB deletion did not change effects of acRoots on hyposensitive cells. Thus, it seems that RARB as the core target within metabolism-associated networks plays important roles in the regulation of lung cancer cell sensitivity to acRoots.

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