Ex Vivo Measurement of Postmortem Tissue Changes in the Crystalline Lens by Brillouin Spectroscopy and Confocal Reflectance Microscopy

2012 ◽  
Vol 59 (8) ◽  
pp. 2348-2354 ◽  
Author(s):  
Stephan Reiss ◽  
K. Sperlich ◽  
M. Hovakimyan ◽  
P. Martius ◽  
R. F. Guthoff ◽  
...  
Keyword(s):  
Ex Vivo ◽  
Crystalline Lens ◽  
Brillouin Spectroscopy ◽  
Confocal Reflectance Microscopy ◽  
Tissue Changes ◽  
Reflectance Microscopy

Immediate ex-vivo diagnosis of pituitary adenomas using confocal reflectance microscopy: a proof-of-principle study

2018 ◽  
Vol 128 (4) ◽  
pp. 1072-1075 ◽  
Author(s):  
Michael A. Mooney ◽  
Joseph Georges ◽  
Mohammedhassan Izady Yazdanabadi ◽  
Katherine Y. Goehring ◽  
William L. White ◽  
...  
Keyword(s):  
Pituitary Adenoma ◽  
Pituitary Adenomas ◽  
Ex Vivo ◽  
Frozen Section ◽  
Surgical Biopsy ◽  
Confocal Reflectance Microscopy ◽  
Normal Gland ◽  
Pathology Department ◽  
Biopsy Specimens ◽  
Reflectance Microscopy

OBJECTIVEThe objective of this study was to evaluate the feasibility of using confocal reflectance microscopy (CRM) ex vivo to differentiate adenoma from normal pituitary gland in surgical biopsy specimens. CRM allows for rapid, label-free evaluation of biopsy specimens with cellular resolution while avoiding some limitations of frozen section analysis.METHODSBiopsy specimens from 11 patients with suspected pituitary adenomas were transported directly to the pathology department. Samples were immediately positioned and visualized with CRM using a confocal microscope located in the same area of the pathology department where frozen sections are prepared. An H & E–stained slide was subsequently prepared from imaged tissue. A neuropathologist compared the histopathological characteristics of the H & E–stained slide and the matched CRM images. A second neuropathologist reviewed images in a blinded fashion and assigned diagnoses of adenoma or normal gland.RESULTSFor all specimens, CRM contrasted cellularity, tissue architecture, nuclear pleomorphism, vascularity, and stroma. Pituitary adenomas demonstrated sheets and large lobules of cells, similar to the matched H & E–stained slides. CRM images of normal tissue showed scattered small lobules of pituitary epithelial cells, consistent with matched H & E–stained images of normal gland. Blinded review by a neuropathologist confirmed the diagnosis in 15 (94%) of 16 images of adenoma versus normal gland.CONCLUSIONSCRM is a simple, reliable approach for rapidly evaluating pituitary adenoma specimens ex vivo. This technique can be used to accurately differentiate between pituitary adenoma and normal gland while preserving biopsy tissue for future permanent analysis, immunohistochemical studies, and molecular studies.

scholarly journals Immediate Label-Free Ex Vivo Evaluation of Human Brain Tumor Biopsies With Confocal Reflectance Microscopy

2017 ◽  
Vol 76 (12) ◽  
pp. 1008-1022 ◽  
Author(s):  
Jennifer M. Eschbacher ◽  
Joseph F. Georges ◽  
Evgenii Belykh ◽  
Mohammedhassan Izady Yazdanabadi ◽  
Nikolay L. Martirosyan ◽  
...  
Keyword(s):  
Brain Tumor ◽  
Human Brain ◽  
Ex Vivo ◽  
Label Free ◽  
Human Brain Tumor ◽  
Confocal Reflectance Microscopy ◽  
Tumor Biopsies ◽  
Reflectance Microscopy

A novel, noninvasive imaging technique for intraoperative assessment of parathyroid glands: Confocal reflectance microscopy

Surgery ◽  
2000 ◽  
Vol 128 (6) ◽  
pp. 1088-1101 ◽  
Author(s):  
W.Matthew White ◽  
Gary J. Tearney ◽  
Ben Z. Pilch ◽  
Richard L. Fabian ◽  
R.Rox Anderson ◽  
...  
Keyword(s):  
Imaging Technique ◽  
Noninvasive Imaging ◽  
Parathyroid Glands ◽  
Confocal Reflectance Microscopy ◽  
Intraoperative Assessment ◽  
Noninvasive Imaging Technique ◽  
Reflectance Microscopy

scholarly journals Live Cell Refractometry Using Hilbert Phase Microscopy and Confocal Reflectance Microscopy†

2009 ◽  
Vol 113 (47) ◽  
pp. 13327-13330 ◽  
Author(s):  
Niyom Lue ◽  
Wonshik Choi ◽  
Gabriel Popescu ◽  
Zahid Yaqoob ◽  
Kamran Badizadegan ◽  
...  
Keyword(s):  
Live Cell ◽  
Phase Microscopy ◽  
Confocal Reflectance Microscopy ◽  
Reflectance Microscopy

scholarly journals Imaging and Quantification of Myelin Integrity After Injury With Spectral Confocal Reflectance Microscopy

2019 ◽  
Vol 12 ◽  
Author(s):  
David G. Gonsalvez ◽  
SangWon Yoo ◽  
Jessica L. Fletcher ◽  
Rhiannon J. Wood ◽  
Georgina A. Craig ◽  
...  
Keyword(s):  
Confocal Reflectance Microscopy ◽  
Reflectance Microscopy

Radiofrequency thermal ablation on ex-vivo swine thyroids: Morphometrical analysis of tissue changes

2019 ◽  
Vol 166 ◽  
pp. 121
Author(s):  
P. Pregel ◽  
E. Scala ◽  
M. Bullone ◽  
L. Nozza ◽  
R. Garberoglio ◽  
...  
Keyword(s):  
Thermal Ablation ◽  
Ex Vivo ◽  
Radiofrequency Thermal Ablation ◽  
Morphometrical Analysis ◽  
Tissue Changes

Detection of diaminobenzidine reactions using scanning laser confocal reflectance microscopy.

1989 ◽  
Vol 37 (12) ◽  
pp. 1761-1765 ◽  
Author(s):  
J M Robinson ◽  
B E Batten
Keyword(s):  
Confocal Microscopy ◽  
Horseradish Peroxidase ◽  
Scanning Laser ◽  
Reaction Products ◽  
Laser Confocal Microscopy ◽  
Confocal Reflectance Microscopy ◽  
Mouse Oocytes ◽  
Important Addition ◽  
Reflectance Microscopy ◽  
Cytochemical Technique

We used scanning laser confocal microscopy to visualize sites of peroxidatic activity as detected by the diaminobenzidine (DAB) reaction. Imaging was achieved by employing the reflectance mode of this instrument. Intense reflectance was detected after DAB localization of endogenous granule-associated myeloperoxidase in neutrophils and of the exogenous tracer horseradish peroxidase in mouse oocytes. Detection of DAB reaction products with confocal reflectance microscopy will probably be an important addition to the utility of this cytochemical technique.

Sign in / Sign up

Export Citation Format

Share Document