scholarly journals A Highly Sensitive Rapid Diagnostic Test for Chagas Disease That Utilizes a Recombinant Trypanosoma cruzi Antigen

2011 ◽  
Vol 58 (3) ◽  
pp. 814-817 ◽  
Author(s):  
C A Barfield ◽  
R S Barney ◽  
C H Crudder ◽  
J L Wilmoth ◽  
D S Stevens ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Chagas Disease ◽  
Highly Sensitive

scholarly journals A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

PLoS ONE ◽  
2020 ◽  
Vol 15 (1) ◽  
pp. e0227828 ◽  
Author(s):  
Mairi C. W. McClean ◽  
Tapan Bhattacharyya ◽  
Pascal Mertens ◽  
Niamh Murphy ◽  
Quentin Gilleman ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Reservoir Hosts

scholarly journals Analytical Evaluation of Dried Blood Spot and Rapid Diagnostic Test as a New Strategy for Serological Community Screening for Chronic Chagas Disease

2021 ◽  
Vol 11 ◽  
Author(s):  
Aroa Silgado ◽  
Lídia Gual-Gonzalez ◽  
Adrián Sánchez-Montalvá ◽  
Inés Oliveira-Souto ◽  
Lidia Goterris ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Chagas Disease ◽  
Latin American ◽  
Whole Blood ◽  
Enzyme Linked Immunosorbent Assay ◽  
Lower Sensitivity ◽  
Blood Samples ◽  
Screening Programs ◽  
Chronic Chagas Disease

BackgroundChagas disease is a public health problem not only in Latin America, but also in other regions, including Spain, due to migration movements. Conventional serological diagnosis requires an invasive sample (plasma or serum) and a well-equipped laboratory. To circumvent those limitations, blood samples dried on filter paper (DBS) or Rapid Diagnostic Test (RDT) could be a practical alternative to reference protocol for serological screening in epidemiological studies. We evaluated the usefulness of dried blood sampling and a rapid diagnostic test (Trypanosoma Detect™) for the detection of antibodies against T. cruzi for their use in community-based screening.Methodology/Principal FindingsA total of 162 stored paired whole-blood and serum samples from Latin American migrants and 25 negative-control blood samples were included. Diagnosis of chronic Chagas disease was performed in serum according to WHO algorithms. Blood samples were retrospectively collected as dried spots and then analyzed using two different serological techniques, enzyme-linked immunosorbent assay (ELISA) and electrochemiluminescence immunoassay (E-CLIA). Whole-blood samples were also used to evaluate a rapid diagnostic test based on immunochromatography. A better correlation with conventional serum was observed in dried blood elutes using E-CLIA than ELISA (97% vs. 77% sensitivity, respectively). Both assays reported 100% specificity. The median cut-off index values of E-CLIA for dried blood were significantly lower than those for serum (138.1 vs. 243.3, P<0.05). The Trypanosoma Detect™ test presented a sensitivity and specificity of 89.6% and 100%, respectively.ConclusionsThe detection of antibodies against T. cruzi in dried blood samples shows a higher sensitivity when using E-CLIA compared with ELISA. Trypanosoma Detect™ is easier to use but has a lower sensitivity. Hence, we propose a sequential strategy based on performing the rapid test first, and a negative result will be confirmed by DBS-ECLIA for use in community Chagas disease screening programs.

scholarly journals Correction: A lineage-specific rapid diagnostic test (Chagas Sero K-SeT) identifies Brazilian Trypanosoma cruzi II/V/VI reservoir hosts among diverse mammalian orders

PLoS ONE ◽  
2020 ◽  
Vol 15 (4) ◽  
pp. e0231566 ◽  
Author(s):  
Mairi C. W. McClean ◽  
Tapan Bhattacharyya ◽  
Pascal Mertens ◽  
Niamh Murphy ◽  
Quentin Gilleman ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Reservoir Hosts

scholarly journals Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Valérie Briand ◽  
Gilles Cottrell ◽  
Nicaise Tuike Ndam ◽  
Xavier Martiáñez-Vendrell ◽  
Bertin Vianou ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Parasite Density ◽  
Quantitative Polymerase Chain Reaction ◽  
Suspension Array ◽  
Increased Risk ◽  
Array Technology ◽  
Highly Sensitive ◽  
Polymerase Chain

Abstract Background While sub-microscopic malarial infections are frequent and potentially deleterious during pregnancy, routine molecular detection is still not feasible. This study aimed to assess the performance of a Histidine Rich Protein 2 (HRP2)-based ultrasensitive rapid diagnostic test (uRDT, Alere Malaria Ag Pf) for the detection of infections of low parasite density in pregnant women. Methods This was a retrospective study based on samples collected in Benin from 2014 to 2017. A total of 942 whole blood samples collected in 327 women in the 1st and 3rd trimesters and at delivery were tested by uRDT, conventional RDT (cRDT, SD BIOLINE Malaria Ag Pf), microscopy, quantitative polymerase chain-reaction (qPCR) and Luminex-based suspension array technology targeting P. falciparum HRP2. The performance of each RDT was evaluated using qPCR as reference standard. The association between infections detected by uRDT, but not by cRDT, with poor maternal and birth outcomes was assessed using multivariate regression models. Results The overall positivity rate detected by cRDT, uRDT, and qPCR was 11.6% (109/942), 16.2% (153/942) and 18.3% (172/942), respectively. Out of 172 qPCR-positive samples, 68 were uRDT-negative. uRDT had a significantly better sensitivity (60.5% [52.7–67.8]) than cRDT (44.2% [36.6–51.9]) and a marginally decreased specificity (93.6% [91.7–95.3] versus 95.7% [94.0–97.0]). The gain in sensitivity was particularly high (33%) and statistically significant in the 1st trimester. Only 28 (41%) out of the 68 samples which were qPCR-positive, but uRDT-negative had detectable but very low levels of HRP2 (191 ng/mL). Infections that were detected by uRDT but not by cRDT were associated with a 3.4-times (95%CI 1.29–9.19) increased risk of anaemia during pregnancy. Conclusions This study demonstrates the higher performance of uRDT, as compared to cRDTs, to detect low parasite density P. falciparum infections during pregnancy, particularly in the 1st trimester. uRDT allowed the detection of infections associated with maternal anaemia.

scholarly journals trans-Sialidase Inhibition Assay, a Highly Sensitive and Specific Diagnostic Test for Chagas' Disease

2001 ◽  
Vol 8 (1) ◽  
pp. 187-189 ◽  
Author(s):  
A. S. Buchovsky ◽  
O. Campetella ◽  
G. Russomando ◽  
L. Franco ◽  
R. Oddone ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Chagas Disease ◽  
Cross Reaction ◽  
Inhibition Assay ◽  
Highly Sensitive ◽  
Specific Diagnostic Test

ABSTRACT For the diagnosis of Chagas' disease, thetrans-sialidase inhibition assay was able to resolve the results for samples with borderline results, to detect as positive 60% of samples that were negative by conventional serology, and to discriminate idiopathic from chagasic megaviscera or cardiopathy. No cross-reaction with sera from patients with other diseases was observed.

scholarly journals Field performance of the malaria highly sensitive rapid diagnostic test in a setting of varying malaria transmission

2019 ◽  
Vol 18 (1) ◽  
Author(s):  
Julia Mwesigwa ◽  
Hannah Slater ◽  
John Bradley ◽  
Binta Saidy ◽  
Fatima Ceesay ◽  
...  
Keyword(s):  
Malaria Transmission ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Field Performance ◽  
Highly Sensitive

scholarly journals Correction to: Prevalence and clinical impact of malaria infections detected with a highly sensitive HRP2 rapid diagnostic test in Beninese pregnant women

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Valérie Briand ◽  
Gilles Cottrell ◽  
Nicaise Tuikue Ndam ◽  
Xavier Martiáñez–Vendrell ◽  
Bertin Vianou ◽  
...  
Keyword(s):  
Pregnant Women ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Clinical Impact ◽  
Highly Sensitive

An amendment to this paper has been published and can be accessed via the original article.

scholarly journals Severity of Chagasic Cardiomyopathy Is Associated With Response to a Novel Rapid Diagnostic Test for Trypanosoma cruzi TcII/V/VI

2018 ◽  
Vol 67 (4) ◽  
pp. 519-524 ◽  
Author(s):  
Tapan Bhattacharyya ◽  
Louisa A Messenger ◽  
Caryn Bern ◽  
Pascal Mertens ◽  
Quentin Gilleman ◽  
...  
Keyword(s):  
Trypanosoma Cruzi ◽  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Chagasic Cardiomyopathy

scholarly journals Use of a highly-sensitive rapid diagnostic test to screen for malaria in pregnancy in Indonesia

2020 ◽  
Vol 19 (1) ◽  
Author(s):  
Vera T. Unwin ◽  
Rukhsana Ahmed ◽  
Rintis Noviyanti ◽  
Agatha M. Puspitasari ◽  
Retno A. S. Utami ◽  
...  
Keyword(s):  
Diagnostic Test ◽  
Rapid Diagnostic Test ◽  
Malaria In Pregnancy ◽  
Highly Sensitive ◽  
In Pregnancy
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